Abstract
Obstruction of shunt catheters is one of the main causes of shunt malfunction. The fragments of shunts removed from five patients were examined using scanning electron microscopy with a (SEM) JEOL JSM-6390 LV microscope. Fifteen catheters from the brain ventricle, lumbar space, and peritoneal space were studied. SEM studies showed that the catheters’ surfaces were not sufficiently smooth. The inner surface was often covered by a web of collagen fibrils. Aggregates of red and white blood cells, platelets, lymphocytes, mast cells, and macrophages were trapped in the collagen web. Such cellular aggregates formed a coherent, delicate web mainly consisting of ultrastructurally unchanged cellular elements and were well preserved. Other types of aggregates contained completely destroyed cells that appeared to be submerged in thick collagen web fibrils.
We also found a few ultrastructural abnormalities among morphologically unchanged cellular elements. The presence of abnormal red cells showing unusual variability in their shape and size including spherocytosis (thickened, spheroid, and crenate red cells), elliptocytosis (elongated, rod-shaped, or tear-drop red cells), the thalassaemic phenotype of red cells (with inclusion of precipitated unstable hemoglobin in the form of Heinz bodies distorting the red cells, leading to their lysis) was a striking finding. Under scanning electron microscopy, we also recognized swollen or crumpled red cells that looked like potato crisps. Aggregation of thickened blood platelets and white cells was observed frequently. Our study confirmed the importance of the smoothness of the inner surface of the catheter. Smoothness can prevent the formation of cell and protein deposits.
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Bayston R, Ashraf W, Bhundia C (2004) Mode of action of an antimicrobial material for use in hydrocephalus shunt. J Antimicrob Chemother 53:778–782
Brydon HL, Keir G, Thompson EJ, Bayston R, Hayward R, Harkness W (1998) Protein adsorption to hydrocephalus shunts catheters: CSF protein adsorption. J Neurosurg Neuropsychiatry 64:643–697
Carrell RW (2005) Cell toxicity and conformational disease. Trends Cell Biol 15:574–580
Clarke JA, Salsbury AJ (1967) Surface ultrastructure of human blood cells. Nature 215:402–404
Davis LE, Cook G, Costerton W (2002) Biofilm on ventriculoperitoneal shunt tubing as a cause of treatment failure on coccoidioidal meningitis. Research. 8(4):376–379
Kockro RA, Hampl JA, Jansen B, Peters G, Scheiling M, Giaconelli R, Kunze S, Aschoff A (2000) Use of scanning electron microscopy to investigate the prophylactic efficacy of rifampin-impregnated CSF shunt catheters. J Med Microbiol 49:441–450
Salsbury AJ, Clarke JA (1967) New method for detecting changes in the surface appearance of human red blood cells. J Clin Pathol 20:603–610
Tokunaga J, Fujita T, Hattori A (1969) Scanning electron microscopy of normal and pathological human erythrocytes. Arch Histol Jap 31:21–35
Williamson D (1993) The unstable haemoglobins. Blood Rev 7:146–163
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Czernicki, Z., Strzałkowski, R., Walasek, N., Gajkowska, B. (2010). What Can Be Found Inside Shunt Catheters. In: Czernicki, Z., Baethmann, A., Ito, U., Katayama, Y., Kuroiwa, T., Mendelow, D. (eds) Brain Edema XIV. Acta Neurochirurgica Supplementum, vol 106. Springer, Vienna. https://doi.org/10.1007/978-3-211-98811-4_13
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DOI: https://doi.org/10.1007/978-3-211-98811-4_13
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