Abstract
For many studies it is necessary to vary the temperature of the sample. Temperature control may be needed to hold a sample in a well defined state of equilibrium or to study at a series of temperatures. Cryogenic temperatures may be needed for transition metal ions with short relaxation times such that lines are too broad to detect at 298 K. To obtain quantitative results in variable temperature (VT) studies, sample preparation and careful temperature control are important.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Alaouie, A.M., Smirnov, A.I.: Ultra-stable temperature control in EPR experiments: thermodynamics of gel-to-liquid phase transition in spin-labeled phospholipid bilayers and bilayer perturbations by spin labels. J. Magn. Reson. 182, 229–238 (2006)
Crippa, P.R., Urbinati, E.U., Vecli, A.: On the determination of the unpaired spin number by electron spin resonance. J. Phys. E. 4, 1071–1073 (1971)
Dashnau, J.L., Zelent, B., Vanderkooi, J.M.: Tryptophan interactions with glycerol/water and trehalose/sucrose cryosolvents: infrared and fluorescence spectroscopy and ab initio calculations. Biophys. Chem. 114, 71–83 (2005)
Drago, R.S.: Physical Methods in Chemistry. Saunders, USA (1977)
Eaton, S.S., Eaton, G.R.: Relaxation times of organic radicals and transition metal ions. In: Berliner, L.J., Eaton, S.S., Eaton, G.R. (eds.) Distance Measurements in Biological Systems by EPR. Biological Magnetic Resonance, vol. 19, pp. 29–154. Kluwer Academic/Plenum, New York (2000b)
Hagen, W.R., Wassink, H., Eady, R.R., Smith, B.E., Haaker, H.: Quantitative EPR of an S = 7/2 system in thionine-oxidized MeFe proteins of nitrogenase. A redefinition of the P-cluster concept. Eur. J. Biochem. 169, 457–465 (1987)
Pierce, B.S., Elgren, T.E., Hendrich, M.P.: Mechanistic implications for the formation of the diiron cluster in ribonucleotide reductase provided by quantitative EPR spectroscopy. J. Am. Chem. Soc. 125, 8748–8759 (2003)
Swallen, S.F., Kearns, K.L., Mapes, M.K., Kim, Y.S., McMahon, R., Ediger, M.D., Wu, T., Yu, L., Satija, S.: Organic glasses with exceptional thermodynamic and kinetic stability. Science 315, 353–356 (2006)
Wada, T., Yamanaka, M., Fujihara, T., Miyazato, Y., Tanaka, K.: Experimental and theoretical evaluation of the charge distribution over the ruthenium and dioxolene framework of [Ru(OAc)(dioxolene)(terpy)] (terpy) 2, 2':6', 2″-terpyridine) depending on the substituents. Inorg. Chem. 45, 8887–8894 (2006)
Wright, A.C., Song, H.K., Wehrli, F.W.: In vivo MR micro imaging with conventional radiofrequency coils cooled to 77o K. Magn. Reson. Med. 43, 163–169 (2000)
Zecevic, A., Eaton, G.R., Eaton, S.S., Lindgren, M.: Dephasing of electron spin echoes for nitroxide radicals in glassy solvents by non-methyl and methyl protons. Mol. Phys. 95(6), 1255–1263 (1998)
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer-Verlag/Wien
About this chapter
Cite this chapter
Eaton, G.R., Eaton, S.S., Barr, D.P., Weber, R.T. (2010). Temperature. In: Quantitative EPR. Springer, Vienna. https://doi.org/10.1007/978-3-211-92948-3_9
Download citation
DOI: https://doi.org/10.1007/978-3-211-92948-3_9
Published:
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-92947-6
Online ISBN: 978-3-211-92948-3
eBook Packages: Physics and AstronomyPhysics and Astronomy (R0)