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AAV-GAD gene for rat models of neuropathic pain and Parkinson’s disease

  • Conference paper
Reconstructive Neurosurgery

Part of the book series: Acta Neurochirurgica Supplementum ((NEUROCHIRURGICA,volume 101))

Abstract

The introduction of therapeutic genes to neurons by genetic modification has potential as an effective treatment for CNS disorders for all that a successful clinical application has not yet been fully implemented. In this paper, we will discussed the role of AAV vectors with the GAD65 gene for animal models of PD and neuropathic pain. AAV vector is one of the most attractive gene delivery vehicles for direct introduction of therapeutic genes into the CNS in the treatment of neurological diseases. GAD65 is present as a membrane-associated form in synapses and is primarily involved in producing synaptic gamma-aminobutyric acid (GABA) for vesicular release. We constructed rAAV-GAD65 expressing rat GAD65 and demonstrated that rat Parkinsonian symptoms can be significantly improved concomitantly with the production of GAD65. We also demonstrated rAAV-GAD65 as a successful gene delivery vehicle in a chronic pain model by administrating rAAV-GAD65 to DRGs because GAB A driven by GAD is a major inhibitory neurotransmitter in the dorsal horn of the spinal cord and also plays an important role in the ventral horn. We believe that AAV vectors can be excellent candidates for gene therapy of neurological diseases.

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Author information

Authors and Affiliations

  1. Department of Neurosurgery, Yonsei University College of Medicine, CPO Box 8044, Seoul, Korea

    J. Kim, Y. S. Yoon & Jin Woo Chang M.D.

  2. Brain Korea 21 Project for Medical Science & Brain Research Institute, Yonsei University College of Medicine, Seoul, Korea

    J. Kim & Jin Woo Chang M.D.

  3. Department of Microbiology, University of Ulsan College of Medicine, Seoul, Korea

    H. Lee

  4. Research Institute for Biomacromolecules, University of Ulsan College of Medicine, Seoul, Korea

    H. Lee

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  1. J. Kim
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  2. Y. S. Yoon
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  3. H. Lee
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  4. Jin Woo Chang M.D.
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Editor information

Editors and Affiliations

  1. Department of Neurosurgery, Municipal Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan, R.O.C.

    Wen-Ta Chiu

  2. Department of Neurosurgery, Taipei Medical University Hospital, Taipei Medical University, Taipei, Taiwan, R.O.C.

    Yung-Hsiao Chiang

  3. Department of Neurosurgery, National Taiwan University Hospital, National Taiwan University, Taipei, Taiwan, R.O.C.

    Ming-Chien Kao  & Ching-Chang Hung  & 

  4. Center for Neuropsychiatry, China Medical University Hospital, China Medical University, Taichung, Taiwan, R.O.C.

    Shinn-Zong Lin

  5. Department of Neurosurgery, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan, R.O.C.

    Han-Jung Chen

  6. Department of Rehabilitation Medicine, Chang Gung Memorial Hospital, Chang Gung University, Taipei, Taiwan, R.O.C.

    Simon F. T. Tang

  7. National Institute on Drug Abuse, National Institute of Health, Baltimore, USA

    Barry J. Hoffer

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© 2008 Springer-Verlag

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Kim, J., Yoon, Y.S., Lee, H., Chang, J.W. (2008). AAV-GAD gene for rat models of neuropathic pain and Parkinson’s disease. In: Chiu, WT., et al. Reconstructive Neurosurgery. Acta Neurochirurgica Supplementum, vol 101. Springer, Vienna. https://doi.org/10.1007/978-3-211-78205-7_17

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  • DOI: https://doi.org/10.1007/978-3-211-78205-7_17

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-78204-0

  • Online ISBN: 978-3-211-78205-7

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