Abstract
Background
Components of hemolysate, released from hematopoetic cells following SAH, may contribute to vasospasm. Adenosine triphosphate (ATP), adenosine diphosphate (ADP), uridine triphosphate (UTP), and uridine diphosphate (UDP), found in erythrocytes and platelets, have been shown, in vitro, to contract cerebral arteries.
Method
Mice were anaesthetized, the cisterna magna exposed and punctured to inject 40 µl of artificial CSF or nucleotide solution ATPgammaS, ADPbetaS, UTP, or UDP at concentrations of 5 µM, 15 µM, or 45 µM. Mice were re-anaesthetized 12 h post-surgery and perfused prior to arterial casting with a gelatin and India ink mixture. Using a video linked microscope, the diameter of the middle cerebral artery (MCA), anterior cerebral artery (ACA), and internal carotid artery (ICA) were measured.
Findings
ADPbetaS is the only nucleotide causing dose-dependant vasospasm, and at the 45 mM concentration, it is the only nucleotide able to cause statistically significant vasospasm (p<0.05) in all three vessels simultaneously.
Conclusions
Using an in vivo mouse model, we found that ADPbetaS is the most effective nucleotide in producing vasospasm, reaching statistical significance at the 45 µM concentration.
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© 2008 Springer-Verlag
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Jabre, A. et al. (2008). Nucleotide-induced cerebral vasospasm in an in vivo mouse model. In: Kırış, T., Zhang, J.H. (eds) Cerebral Vasospasm. Acta Neurochirurgica Supplement, vol 104. Springer, Vienna. https://doi.org/10.1007/978-3-211-75718-5_15
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DOI: https://doi.org/10.1007/978-3-211-75718-5_15
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