Abstract
One type of future, improved neural interfaces is the ‘cultured probe’. It is a hybrid type of neural information transducer or prosthesis, for stimulation and/or recording of neural activity. It would consist of a micro-electrode array (MEA) on a planar substrate, each electrode being covered and surrounded by a local circularly confined network (‘island’) of cultured neurons. The main purpose of the local networks is that they act as bio-friendly intermediates for collateral sprouts from the in vivo system, thus allowing for an effective and selective neuron electrode interface. As a secondary purpose, one may envisage future information processing applications of these intermediary networks.
In this chapter, first, progress is shown on how substrates can be chemically modified to confine developing networks, cultured from dissociated rat cortex cells, to ‘islands’ surrounding an electrode site. Additional coating of neurophobic, polyimide coated substrate by tri-block-copolymer coating enhances neurophilic-neurophobic adhesion contrast. Secondly, results are given on neuronal activity in patterned, unconnected and connected, circular ‘island’ networks. For connected islands, the larger the island diameter (50, 100 or 150 mm), the more spontaneous activity is seen. Also, activity may show a very high degree of synchronization between two islands. For unconnected islands, activity may start at 22 days in vitro (DIV), which is two weeks later than in unpatterned networks.
This paper has been published under the title ‘Cultured neural networks: optimization of patterned networks adhesiveness and characterization of their neural activity’. In: Applied Bionics & Biomechanics 3(1) (2006), pp. 1–7, DOI 10.1533/abbi.2005.0027; with permission.
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Buitenweg JR, Rutten WLC, Marani E (2003) Geometry based finite-element modeling of the electrical contact between a culture neuron and a microelectrode. IEEE Trans Biomed Eng 50: 501–510
Buitenweg JR, Rutten WLC, Marani E, Polman SKL, Ursum J (2002) Extracellular detection of active membrane currents in the neuron-electrode interface. J Neurosci Methods 115: 211–221
Buitenweg JR, Rutten WLC, Marani E (2002) Extracellular stimulation window explained by a geometry-based model of the neuronelectrode contact. IEEE Trans Biomed Eng 49: 1591–1600
Buitenweg JR, Rutten WLC, Marani E (2002) Modeled channel distributions explain extracellular recordings from cultured neurons sealed to microelectrodes. IEEE Trans Biomed Eng 49: 1580–1591
Ruardij TG, Goedbloed MH, Rutten WLC (2000) Adhesion and patterning of cortical neurons on polyethylenimine and fluorocarbon-coated surfaces. IEEE Trans Biomed Eng 47: 1593–1599
Ruardij TG, Goedbloed MH, Rutten WLC (2003) Long-term adhesion and survival of dissociated cortical neurons on miniaturized chemical patterns. Med Biol Eng Comp 41: 227–232
Corner MA, Ramakers GJA (1992) Spontaneous firing as an epigenetic factor in brain development — physiological consequences of chronic tetrodotoxin and picrotoxin exposure in cultured rat neocortex neurons. Dev Brain Res 65: 57–64
Gross G (1979) Simultaneous single unit recording in vitro with a photoetched laser deinsulated gold multielectrode surface. IEEE Trans Biomed Eng 26: 273–278
Pine J (1980) Recording action potentials from cultured neurons with extracellular microcircuit electrodes. J Neurosci Meth 2: 19–31
Jimbo Y, Tateno T, Robinson HPC (1999) Simultaneous induction of pathway-specific potentiation and depression in networks of cortical neurons. Biophys J 76: 670–678
Corey JM, Wheeler BC, Brewer GJ (1996) Micrometer resolution silane-based patterning of hippocampal neurons: critical variables in photoresist and laser ablation processes for substrate fabrication. IEEE Trans Biomed Eng 43: 944–955
Ruardij TG, van den Boogaart MAF, Rutten WLC (2002) Adhesion and growth of electrically-active cortical neurons on polyethyleneimine patterns microprinted on PEO-PPO-PEO triblockcopolymer-coated hydrophobic surfaces. IEEE Trans Nanobiosci 1: 1–8
van Staveren GW, Buitenweg JR, Heida T, Rutten WLC (2002) Wave shape classification of spontaneous neuronal activity in cortical cultures on micro-electrode arrays. Proceedings second joint IEEE-EMBS/BMES conference, Houston USA, ISBN: 0-7803-7613-7 CD-ROM
van Pelt J, Wolters PS, Corner MA, Rutten WLC, Ramakers GJA (2004) Long-term characterisation of firing dynamics of spontaneous bursts in cultured neural networks. IEEE Trans Biomed Eng 51: 2051–2062
Chang JC, Brewer GJ, Wheeler BC (2001) Modulation of neural network activity by patterning. Biosens Bioelectr 16: 527–533
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© 2007 Springer-Verlag
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Rutten, W.L.C., Ruardij, T.G., Marani, E., Roelofsen, B.H. (2007). Neural networks on chemically patterned electrode arrays: towards a cultured probe. In: Sakas, D.E., Simpson, B.A. (eds) Operative Neuromodulation. Acta Neurochirurgica Supplements, vol 97/2. Springer, Vienna. https://doi.org/10.1007/978-3-211-33081-4_63
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DOI: https://doi.org/10.1007/978-3-211-33081-4_63
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