Abstract
In this study, we examine 3CB2 expression, a marker of radial glia, after intracerebral hemorrhage (ICH). Adult male Sprague-Dawley rats received an intracaudate injection of 100 mL autologous whole blood. Animals were sacrificed, and 3CB2 expression was quantified on Western blot. Single and double labeled immunohistochemistry was used to identify which cells express 3CB2. Neurobehavioral examinations (forelimb placing test) were perfomed as an evaluation of function. By Western blot, 3CB2 was strongly expressed at day 3 and expression persisted for at least 1 month. By immunohistochemistry, 3CB2 immu-noreactivity was present in large numbers of astrocytes surrounding the hematoma at day 3 after ICH. At 1 month later, 3CB2 immunoreactivity was co-localized with a neuronal marker (TUC-4). Neurobehavioral function in the 1 month after ICH group was significantly improved compared with that of 3 days after ICH. The ICH-induced 3CB2 expression in astrocytes may reflect an early response of these cells to injury, while the delayed expression in neurons might be a part of the adaptative response to injury, perhaps leading to recovery of neurobehavioral function.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Englund U, Björklund A, Wictorin K (2002) Migration patterns and phenotypic differentiation of long-term expanded human neural progenitor cells after transplantation into the adult rat brain. Brain Res Dev Brain Res 134: 123–141
Hua Y, Schallert T, Keep RF, Wu J, Hoff JT, Xi G (2002) Behavioral tests after intracerebral hemorrhage in the rat. Stroke 33: 2478–2484
McGraw J, Hiebert GW, Steeves JD (2001) Modulating astrogliosis after neurotrauma. J Neurosci Res 63: 109–115
Mission JP, Takahashi T, Caviness VS Jr (1991) Ontogeny of radial and other astroglial cells in murine cerebral cortex. Glia 4:138–148
Nakamura T, Xi G, Hua Y, Hoff JT, Keep RF (2003) Nestin expression after experimental intracerebral hemorrhage. Brain Res 981: 108–117
Nakamura T, Miyamoto O, Auer RN, Nagao S, Itano T (2004) Delayed precursor cell markers expression in hippocampus following cold-induced cortical injury in mice. J Neurotrauma 21: 1747–1755
Parnavelas JG, Nadarajah B (2001) Radial glial cells. Are they really glia? Neuron 31: 881–884
Prada FA, Dorado ME, Quesada A, Prada C, Schwarz U, de la Rosa EJ (1995) Early expression of a novel radial glia antigen in the chick embryo. Glia 15: 389–400
Shibuya S, Miyamoto O, Itano T, Mori S, Norimatsu H (2003) Temporal progressive antigen expression in radial glia after con-tusive spinal cord injury in adult rats. Glia 42: 172–183
Tanaka S, Miyamoto O, Janjua NA, Miyazaki T, Takahashi F, Konishi R, Itano T (2005) Stage and region dependent expression of a radial glial marker in commissural fibers in kindled mice. Epilepsy Res 67: 61–72
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2008 Springer-Verlag
About this paper
Cite this paper
Nakamura, T. et al. (2008). Radial glia marker expression following experimental intracerebral hemorrhage. In: Zhou, LF., et al. Cerebral Hemorrhage. Acta Neurochirurgica Supplementum, vol 105. Springer, Vienna. https://doi.org/10.1007/978-3-211-09469-3_19
Download citation
DOI: https://doi.org/10.1007/978-3-211-09469-3_19
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-09468-6
Online ISBN: 978-3-211-09469-3
eBook Packages: MedicineMedicine (R0)