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Effects of aging on complement activation and neutrophil infiltration after intracerebral hemorrhage

  • Conference paper
Book cover Cerebral Hemorrhage

Part of the book series: Acta Neurochirurgica Supplementum ((NEUROCHIRURGICA,volume 105))

Abstract

Intracerebral hemorrhage (ICH)-induced brain edema and neurological deficits are greater in aged rats than in young rats. Complement activation and neutrophil infiltration contribute to brain injury after ICH. In this study, we investigated the effects of aging on activation of the complement cascade and neutrophil influx following ICH.

Male Sprague-Dawley rats (3 or 18 months old) received an infusion of 100 mL autologous blood into right caudate. Rats were killed at 1,3,7, and 28 days after ICH and the brains were sampled for immunohis-tochemistry and Western blot analysis. Levels of complement factor C9 and clusterin were used as markers for complement activation, and myeloperoxidase (MPO) staining was performed to detect neutrophil infiltration. Western blot analysis showed that complement C9 and clusterin levels in ipsilateral basal ganglia after ICH were higher in aged rats than in young rats (p < 0.05). Immunohistochemistry showed there were more C9- and clusterin-positive cells around the hematoma in aged rats. However, MPO-positive cells in ipsilateral basal ganglia were fewer in aged rats (p<0.05) after ICH.

Our results suggest that ICH causes more severe complement activation and less neutrophil infiltration in aged rats. Clarification of the mechanisms of brain injury after ICH in the aging brain should help develop new therapeutic strategies for ICH.

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Author information

Authors and Affiliations

  1. Department of Neurosurgery, University of Michigan Medical School, 109 Zina Pitcher Place, R5018 Biomedical Science Research Building, Ann Arbor, MI, 48109-2200, USA

    Y. Gong, G. Xi, S. Wan, Y. Gu, R. F. Keep & Y. Hua MD

  2. Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China

    Y. Gong & Y. Gu

Authors
  1. Y. Gong
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  2. G. Xi
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  3. S. Wan
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  4. Y. Gu
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  5. R. F. Keep
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  6. Y. Hua MD
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Editor information

Editors and Affiliations

  1. Department of Neurosurgery, School of Medicine, Huashan Hospital, Fudan University, Shanghai, China

    Liang-Fu Zhou , Xian-Cheng Chen  & Feng-Ping Huang ,  & 

  2. Department of Neurosurgery, University of Michigan, Ann Arbor, MI, USA

    Guohua Xi , Richard F. Keep , Ya Hua  & Karin Muraszko , ,  & 

  3. Department of Neurosurgery, Shanghai Changzheng Hospital, Shanghai, China

    Yi-Cheng Lu

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© 2008 Springer-Verlag

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Gong, Y., Xi, G., Wan, S., Gu, Y., Keep, R.F., Hua, Y. (2008). Effects of aging on complement activation and neutrophil infiltration after intracerebral hemorrhage. In: Zhou, LF., et al. Cerebral Hemorrhage. Acta Neurochirurgica Supplementum, vol 105. Springer, Vienna. https://doi.org/10.1007/978-3-211-09469-3_14

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  • DOI: https://doi.org/10.1007/978-3-211-09469-3_14

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-09468-6

  • Online ISBN: 978-3-211-09469-3

  • eBook Packages: MedicineMedicine (R0)

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