Summary
Free amino acids are competitive inhibitors of tyrosine phenol lyase from Citrobacter intermedius The correlation exist between -RTlnKi. and side chain hydrophobicity. Aspartic and glutamic acids are anomalously strong inhibitors taking into consideration low hydrophobicity of their side chains. One can assume that the electrophilic group able to interact with the terminal C00−-group of aspartic and glutamic acids is located in the active site of the enzyme. The substrate specificity of tyrosine phenol-lyase is controlled during the stage of aromatic ring elimination. The OH-group in the p- position of the ring is the first necessary condition for the stage to proceed. The same stage is also sensitive to the steric parameters of the substituent in the ring which ensures the second factor of control.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Davis. L., Metzler, D. E. (1972) In: The Enzymes (P. D. Boyer, Ed.) Academic Press, New York, vol.7, pp. 33–74.
Demidkina, T. V., Myagkikh. I. Y., Faleev, N. G., Belikov, V. M., (1984) Biokhimia (HUBS.) 49, 32–37.
Dunathan, H.C. (1971) Adv.Enzymol. 85, 79–134.
Enei, H., Matsui, H., Yamashita, K., Okumura, Sh., Yamada. H. (1972) Agr.Biol.Chem., 36, 1861–1868.
Faleev, N.G., Lyubarev, ATS., Martinkova N.S., Belikov, V.M.; (1983) Enzyme Microb. Technol. 5, 219–224.
Frömmel, C.J. (1984) J.Theor. Biol. 111, 247–260.
Haash, C., Grieco, C., Silpo, C., Vittoria, A. J.Med. Chem. (1977) 20, 1420–1435.
Johnson, K T Hammet Equation. Moscow: Mir, (1977) p. 48, 62.
Kumagai, H.,Utagawa, T., Yamada, H. J.Biol. Chem. (1975) 250, 1661–1667.
Martinek, K., Levashov. A.V., Berezin, I.V. (1970) Molekularnaya Biologia (Russ.), 4, 517–528.
Nagasawa, T., Utagawa, T., Goto, J., Kin Chan Jo. Tani, J., Kumagai, H., Yamada, H. Eur. J.Biochem. (1981) 117, 33–40.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Birkhäuser Verlag Basel
About this chapter
Cite this chapter
Faleev, N.G. et al. (1987). The Substrate Specificity of Tyrosine Phenol-Lyase During the Different Stages of Enzymatic Process. In: Korpela, T.K., Christen, P. (eds) Biochemistry of Vitamin B6 . Birkhäuser Congress Reports. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-9308-4_39
Download citation
DOI: https://doi.org/10.1007/978-3-0348-9308-4_39
Publisher Name: Birkhäuser, Basel
Print ISBN: 978-3-0348-9989-5
Online ISBN: 978-3-0348-9308-4
eBook Packages: Springer Book Archive