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Branched-Chain Amino Acid Aminotransferase from Escherichia Coli

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Biochemistry of Vitamin B6

Part of the book series: Birkhäuser Congress Reports ((ALS))

Summary

The nucleotide sequence of the E.coli ilvE gene, which encodes branched-chain amino acid aminotranferase (IlvAT), was determined. The enzyme was overproduced by inserting the ilvE gene into a vector with a tac promotor.

The primary, secondary, and quaternary structures of IlvAT were different from those of aspartate aminotransferase (AspAT). The absorption spectrum of the holoenzyme showed no detectable pH-dependent change in the range of pH 5 to 9. Besides branched-chain amino acids, Met, Phe, Tyr, and Trp served as amino donor to 2-oxoglutarate with a lower extent.

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References

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Authors and Affiliations

  1. Department of Medical Chemistry, Osaka Medical College, Daigaku-Cho 2-7, Takatsuki, Osaka, 569, Japan

    S. Kuramitsu, K. Inoue & H. Kagamiyama

Authors
  1. S. Kuramitsu
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  2. K. Inoue
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  3. H. Kagamiyama
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Editor information

Editors and Affiliations

  1. Department of Biochemistry, University of Turku, SF-20500, Turku, Finland

    Timo K. Korpela

  2. Biochemisches Institut, der Universität Zürich, CH-8057, Zürich, Switzerland

    Philipp Christen

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© 1987 Birkhäuser Verlag Basel

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Kuramitsu, S., Inoue, K., Kagamiyama, H. (1987). Branched-Chain Amino Acid Aminotransferase from Escherichia Coli. In: Korpela, T.K., Christen, P. (eds) Biochemistry of Vitamin B6 . Birkhäuser Congress Reports. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-9308-4_27

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  • DOI: https://doi.org/10.1007/978-3-0348-9308-4_27

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-9989-5

  • Online ISBN: 978-3-0348-9308-4

  • eBook Packages: Springer Book Archive

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