Summary
A major product released by heat from the 340 nm chromophore of the beta subform of cytosolic aspartate aminotransferase has been identified conclusively as pyridoxamine 5′-phosphate. The N-phosphopyridoxyl derivative of 5-oxoproline has been identified as a minor product. We suggest that the beta chromophore is a stabilized ketimine that can isomerize to an aldimine and release, in part, pyridoxal phosphate at high pH. It may have arisen from a reactive 5-carbon substrate.
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References
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© 1987 Birkhäuser Verlag Basel
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Metzler, C.M., Metzler, D.E., Jin, P., Likos, J. (1987). Studies on the Inactivated Coenzyme in the Beta Subform of Cytosolic Aspartate Aminotransferase. In: Korpela, T.K., Christen, P. (eds) Biochemistry of Vitamin B6 . Birkhäuser Congress Reports. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-9308-4_19
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DOI: https://doi.org/10.1007/978-3-0348-9308-4_19
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