Summary
A specific antibody against rat dopa decarboxylase (DDC) immunostained only DDC-containing cells both in rat and guinea-pig tissues. On the other hand, a specific anti-rat histidine decarboxylase (HDC) antibody which reacted only with HDC-containing cells in rat tissues immunostained DDC-containing cells also in guinea-pig tissues. On immunoblotting, anti-rat HDC antibody recognized not only rat HDC but also guinea-pig DDC.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ando-Yamamoto, M., Hayashi, H., Taguchi, Y., Fukui, H., Watanabe, T. and Wada H. (1986) Biochem.Biophys.Res.Commun. 141, 306–312.
Ando-Yamamoto, M., Hayashi, H., Sugiyama, T., Fukui, H., Watanabe, T. and Wada H. (1987) J.Biochem. 101, 405–414.
Coons, A.H. (1958) In: General Cytochemical Methods (J.F. Danielli, Ed), Academic Press, New York, pp. 399–422.
Laemmli, U.K. (1970) Nature(Lond.) 227, 680–685.
Taguchi, Y., Watanabe, T., Kubota, H., Hayashi, H. and Wada, H. (1984) J.Biol.Chem. 259, 5214–5221.
Towbin, H., Staehelin, T. and Gordon, H. (1979) Proc.Natl.Acad.Sci.USA. 76, 4350–4354.
Watanabe, T., Taguchi, Y., Shiosaka, S., Tanaka, J., Kubota, H., Terano, Y., Tohyama, M. and Wada, H. (1984) Brain.Res. 295, 13–25.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Birkhäuser Verlag Basel
About this chapter
Cite this chapter
Wada, H., Ando-Yamamoto, M., Hayashi, H., Taguchi, Y., Fukui, H., Watanabe, T. (1987). Demonstration of Immunochemical Cross-Reactivity of Dopa Decarboxylase and Histidine Decarboxylase Using Antibodies Against the Two Enzymes. In: Korpela, T.K., Christen, P. (eds) Biochemistry of Vitamin B6 . Birkhäuser Congress Reports. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-9308-4_11
Download citation
DOI: https://doi.org/10.1007/978-3-0348-9308-4_11
Publisher Name: Birkhäuser, Basel
Print ISBN: 978-3-0348-9989-5
Online ISBN: 978-3-0348-9308-4
eBook Packages: Springer Book Archive