Summary
Acetylcholine (Ach) was tested for its effect on calcium currents in primary cultures of embryonic rat hippocampal neurons. Ach reversibly depressed, in a dose-dependent way, the high voltage activated (HVA) Ca currents. The effect was antagonized by atropine. Our results suggest that a pertussis toxin (PTX)-sensitive GTP binding protein (G-protein) is involved in the signal transduction mechanism between the Ach receptor and the HVA Ca channel. Activating rather than depressive effects of Ach were observed on the low voltage-activated component of Ca currents. This effect was also antagonized by atropine but is not mediated by a PTX-sensitive G-protein.
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References
Carbone, E., and Lux, H. D. (1987) Kinetics and selectivity of a low voltage activated calcium current in chick and rat sensory neurones. J. Physiol. 386: 547–570
Dunlap, K., Holz, G. G., and Rane, S. G. (1987) G proteins as regulators of ion channel function. Trends Neurosci. 10: 241–244
Dutar, P., and Nicoll, R. A. (1988) Stimulation of phosphatidyl inositol turnover may mediate the muscarinic suppression of the M current in hippocampal pyramidal cells. Neurosci. Lett. 85: 89–94
Evans, T., Martin, M., Hughes, A. R., and Harden, T. K. (1985) Guanine nucleotide sensitive, high affinity binding of carbachol to muscarinic cholinergic receptors of 1321N1 astrocytoma cells is insensitive to pertussis toxin. Molec. Pharmac. 27: 32–37
Fisher, S. K., and Bartus, R. T. (1985) Regional differences in the coupling of muscarinic receptors to inositol phospholipid hydrolysis in guinea pig brain. J. Neurochem. 45: 1085–1095
Fox, A. P., Nowycky, M. C., and Tsien, R. W. (1987) Kinetic and pharmacological properties distinguishing three types of calcium currents in chick sensory neurons. J. Physiol. 394: 149–172
Gähwiler, B. H., and Brown, D. A. (1987) Muscarine affects calcium currents in rat hippocampal pyramidal cells in vitro. Neurosci. Lett. 76: 301–306
Ganong, B. R., Loomis, C. R., Hannun, Ya., and Bell, R. M. (1986) Specificity and mechanism of protein kinase C activation by sn-1,2 diacylglycerols. Proc. natl Acad. Sci. 83: 1184–1188
Hamill, O. P., Marty, A., Neher, E., Sakmann, B., and Sigworth, F. J. (1981) Improved patch clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch. 391: 85–100
Hidaka, H., Inagaki, M., Kawamoto, S., and Sasaki, Y. (1984) Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C. Biochemistry 23: 5036–5041
Isom, L. L., Cragoe, E. J., and Limbird, L. E. (1987) Multiple receptors linked to inhibition of adenilate cyclase accelerate Na+/H+ exchange in neuroblastoma x glioma cells via a mechanism other than decreased cAMP accumulation. J. biol. Chem. 262: 17504–17509
Kilbinger, H., and Nafziger, M. (1985) Two types of neuronal muscarine receptors modulating acetylcholine release from guinea pig myenteric plexus. Naunyn-Schmiedeberg Arch. Pharmak. 328: 304–309
Konnerth, A., Lux, H. D., and Morad, M. (1988) Proton induced transformation of calcium channel in chick dorsal root ganglion cells. J. Physiol. 386: 603–633
Lewis, P. R., and Shute, C. C. D. (1967) The cholinergic limbic system: projections to hippocampal formation, medial cortex, nuclei of the ascending cholinergic reticular system, and the subfornical organ and supra-optic crest. Brain 90: 521–540
Llinas, R., and Yarom, Y. (1981) Properties and distribution of ionic conductances generating electroresponsiveness of mammalian inferior olivary neurons in vitro. J. Physiol. 315: 569–584
Malenka, R. C., Madison, D. V., Andrade, R., and Nicoll, R. A. (1986) Phorbol esters mimick some cholinergic actions in hippocampal pyramidal neurons. J. Neurosci. 6: 475–480
McCleskey, E. W., Fox, A. P., Feldman, D. H., Cruz, L. J., Olivera, B. M., Tsien, R. W., and Yoshikami, D. (1987) ?-Conotoxin: direct and persistent blockade of specific types of calcium channels in neurons but not muscle. Proc natl Acad. Sci. 84: 4327–4331
Madison, D. V., and Nicoll, R. A. (1984) Control of the repetitive discharge of rat CA1 pyramidal neurons in vitro. J. Physiol. 354: 319–331
Misgeld, U., Galabresi, P., and Dodt, U. (1986) Muscarinic modulation of calcium-dependent plateau potentials in rat neostriatal neurons. Pflugers Arch. 407: 482–487
Nakajima, Y., Nakajima, S., and Inoue, M. (1988) Pertussis toxin insensitive G protein mediates substance P-induced inhibition of potassium channels in brain neurons. Proc. natl Acad. Sci. 85: 3643–3647
Nathanson, N. M. (1987) Molecular properties of the muscarinic acetylcholine receptor. A. Rev. Neurosci. 10: 195–236
Nishizuka, Y. (1984) The role of protein kinase C in cell surface signal transduction and tumor promotion. Nature 308: 693–698
Pozzan, T., DiVirgilio, F., Vincentini, L. M., and Meldolesi, J. (1986) Activation of muscarinic receptors in PC 12 cells. Biochem. J. 234: 547–553
Toselli, M., and Lux, H. D. (1989) GTP binding proteins mediate acetylcholine inhibition of voltage-dependent calcium channels in hippocampal neurons. Pflügers Arch. 413: 319–321
Wanke, E., Ferroni, A., Malgaroli, A., Ambrosini, A., Pozzan, T., and Meldolesi, J. (1987) Activation of a muscarinic receptor selectively inhibits a rapidly inactivated Ca current in rat sympathetic neurons. Proc. natl Acad. Sci. USA 84: 4313–4317
Weiler, M. H., Misgeld, U., and Cheong, D. K. (1984) Presynaptic muscarinic modulation of nicotinic excitation in the rat neostriatum. Brain Res. 296: 111–120
Yaari, Y., Hamon, B., and Lux, H. D. (1987) Development of two types of calcium channels in cultured mammalian hippocampal neurons. Science 235: 680–682
Yatani, A., Codina, J., Brown, A. M., and Birnbaumer, L. (1987) Direct activation of mammalian atrial muscarinic potassium channels by GTP regulatory protein Gk. Science 235: 207–211
Yatani, A., Codina, J., Imoto, Y., Reeves, J. P., Birnbaumer, L., and Brown, A. M. (1987) A G protein directly regulates mammalian cardiac calcium channels. Science 238: 1288–1292
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© 1989 Birkhäuser Verlag
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Toselli, M., Lux, H.D. (1989). Opposing effects of acetylcholine on the two classes of voltage-dependent calcium channels in hippocampal neurons. In: Frotscher, M., Misgeld, U. (eds) Central Cholinergic Synaptic Transmission. Experientia Supplementum, vol 57. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-9138-7_10
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DOI: https://doi.org/10.1007/978-3-0348-9138-7_10
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