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Determination of 8-oxo-purines in DNA by HPLC using amperometric detection

  • Chapter
Analysis of Free Radicals in Biological Systems

Summary

High-performance liquid chromatography coupled with electrochemical detection is a sensitive assay for 8-oxo-purines derivatives. This technique was applied to the measurement of the rate of formation of 8- oxo-dGuo in photooxidized isolated DNA following either enzymatic digestion or acidic hydrolysis. The assay was also used to assess the excision of 8-oxo-Gua from DNA upon incubation with the Fapy DNA glycosylase protein. It also allowed the determination of the respective yield of 8-oxo-dGuo and 8-oxo-dAdo upon γ-radiolysis of DNA in aqueous aerated solution.

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Author information

Authors and Affiliations

  1. Département de Recherche Fondamentale sur la Matiére Condensée, CEA, SESAM/LAN, F-38054, Grenoble, Cédex 9, France

    T. Douki, M. Berger, S. Raoul, J.-L. Ravanat & J. Cadet

Authors
  1. T. Douki
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  2. M. Berger
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  3. S. Raoul
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  4. J.-L. Ravanat
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  5. J. Cadet
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Editor information

Editors and Affiliations

  1. Laboratoire de Biochimie C, CHU Albert Michallon, BP 217, F-38043, Grenoble Cédex 9, France

    A. E. Favier

  2. CEA/Département de Recherche Fondamentale sur la Matière, Condensée, SESAM/LAN, 17, rue des Martyrs, F-38054, Grenoble Cédex 9, France

    J. Cadet

  3. Biophysics Research Institute, Medical College of Wisconsin, 8701 Watertown Plank Road, PO Box 26509, Milwaukee, WI, 53226-0509, USA

    B. Kalyanaraman

  4. Laboratoire de Biochimie Recherche, Domaine Universitaire, F-38400, Saint Martin d’Hères, France

    M. Fontecave

  5. Laboratoire de Chimie Biomimétique LEDSS II, Domaine Universitaire, F-38400, Saint Martin d’Hères, France

    J.-L. Pierre

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© 1995 Birkhäuser Verlag Basel/Switzerland

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Douki, T., Berger, M., Raoul, S., Ravanat, JL., Cadet, J. (1995). Determination of 8-oxo-purines in DNA by HPLC using amperometric detection. In: Favier, A.E., Cadet, J., Kalyanaraman, B., Fontecave, M., Pierre, JL. (eds) Analysis of Free Radicals in Biological Systems. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-9074-8_15

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  • DOI: https://doi.org/10.1007/978-3-0348-9074-8_15

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-0348-9895-9

  • Online ISBN: 978-3-0348-9074-8

  • eBook Packages: Springer Book Archive

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