Skip to main content
SpringerLink
Log in
Book cover

Frontiers in Biosensorics II pp 27–44Cite as

Biosensors based on flow-through systems

  • Chapter

Part of the book series: EXS ((EXS,volume 81))

Summary

When combined with biosensors as the sensing element microdialysis and flow injection analysis (FIA) systems become sophisticated tools for handling analytical processes. In particular a FIA system offers a high degree of automation together with high reproducibility and small sample volumes, whereas the biosensor, allows selective and sensitive measurements of the various analytes. Here we describe first a miniaturised microdialysis flow-through system developed for glucose determination, then we focus on amperometric immunosensors and on microbial sensors. In the former, antibodies against low molecular weight environmental contaminants or against high molecular weight proteins are responsible for analyte detection, whereas the latter use immobilised microorganisms as the recognising element for monitoring water pollutants.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Blum, L.J., Gautier, S.M. and Coulet, P.R. (1993) Design of bioluminescence-based fiber optic sensors for flow-injection analysis. J. Biotechnol. 31: 357–368.

    Article  PubMed  CAS  Google Scholar 

  • Busch, M., Höbel, W. and Polster, J. (1993) Software FIACRE: Bioprocess monitoring on the basis of flow injection analysis using simultaneously a urea optode and a glucose luminescence sensor. J. Biotechnol. 31: 327–343.

    Article  PubMed  CAS  Google Scholar 

  • Camann, K., Hinkers, H. and Knoll, M. (1994) Microstructures and microsystems in instrumental analysis. Analusis 22: M19 - M21.

    Google Scholar 

  • Céspedes, E, Valero, E, Martinez-Fàbregas, E., Bartroli, J. and Alegret, S. (1995) Fermentation monitoring using a glucose biosensor based on an electrocatalytically bulk-modified epoxy-graphite biocomposite integrated in a flow system. Analyst 120: 2255–2258.

    Article  Google Scholar 

  • DeRiemer, L.H. and Meares, C.F. (1981) Synthesis of mono-and dinucleotide photoaffinity probes of ribonucleic acid polymerase. Biochemistry 20: 1606–1612.

    Article  PubMed  CAS  Google Scholar 

  • Fang, Z. (1993) Flow injection separation and preconcentration. VCH, Weinheim, Germany.

    Google Scholar 

  • Hansen, E.H., Gundstrup, M. and Mikkelsen, H.S. (1993) Determination of minute amounts of ATP by flow injection analysis using enzyme amplification reactions and fluorescence detection. J. Biotechnol. 31: 369–380.

    Article  PubMed  CAS  Google Scholar 

  • Kalàb, T. and Sklàdal, P. (1995) A disposable amperometric immunosensor for 2,4-dichlorophenoxyacetic acid. Anal. Chim. Acta 304: 361–368.

    Article  Google Scholar 

  • Karube, I. and Suzuki, M. (1990) Microbial biosensors. In: A.E.G. Cass (ed.): Biosensors — A Practical Approach. Oxford University Press, Oxford, U.K., pp 155–170.

    Google Scholar 

  • König, A., Zaborosch, C. Muscat, A., Vorlop, K.-D. and Spener, F. (1996) Microbial sensors for naphthalene using Sphingomonas sp. B1 or Pseudomonas fluorescens WW4. Appl. Microbiol. Biotechnol. 45: 844–850.

    Article  Google Scholar 

  • Knoll, M. (1995a) Miniaturisiertes Durchflußanalysesystem. German Patent 441: 224–252.

    Google Scholar 

  • Knoll, M. (1995b). Verfahren zur Herstellung von miniaturisierten Chemo-und Biosensorelementen mit ionenselektiver Membran sowie von Trägern für diese Elemente. German Patent DE 41: 154–141.

    Google Scholar 

  • Manowitz, P., Stoecker, P.W. andYacynych, A.M. (1995) Galactose biosensors using composite polymers to prevent interferences. Biosens. Bioelectron. 10: 359–370.

    Article  CAS  Google Scholar 

  • Meyerhoff, M.E., Duan, C. and Meusel, M. (1995) A novel non-separation sandwich type electrochemical enzyme immunoassay system for detecting marker proteins in undiluted blood. Clin. Chem. 41: 1378–1384.

    PubMed  CAS  Google Scholar 

  • Middendorf, C., Schulze, B., Freitag, R., Scheper, T., Howaldt, M. and Hoffmann, H. (1993) Online immunoanalysis for bioprocess control. J. Biotechnol. 31: 395–403.

    Article  PubMed  CAS  Google Scholar 

  • Mulchandini, A. and Bassi, A.S. (1995) Principles and applications of biosensors for bioprocess monitoring and control. Crit. Rev. Biotechnol. 15: 105–124.

    Article  Google Scholar 

  • Nilsson, M., Mattiasson, G. and Mattiasson, B. (1993) Automated immunochemical binding assay (flow-ELISA) based on repeated use of an antibody column placed in a flow-injection system. J. Biotechnol. 31: 381–394.

    Article  PubMed  CAS  Google Scholar 

  • Preuschoff, F., Spohn, U., Blankenstein, G., Mohr, K.-H. and Kula, M.-R. (1993) Chemiluminometric hydrogen peroxide sensor for flow injection analysis. Fresenius J. Anal. Chem. 246: 924–929.

    Article  Google Scholar 

  • Riedel, K. (1994) Microbial sensors and their applications in environment. Exp. Technique Phys. 40: 63–76.

    CAS  Google Scholar 

  • Riedel, K., Naumov, A.V., Boronin, A.M., Golovleva, L.A., Stein, H.J. and Scheller, F. (1991) Microbial sensors for determination of aromatics and their chloroderivatives I. Determination of 3-chlorobenzoate using a Pseudomonas-containing biosensor. Appl. Microbiol. Biotechnol. 35: 559–562.

    Article  CAS  Google Scholar 

  • Riedel, K., Hensel, J., Rothe, S., Neumann, B and Scheller, F. (1993) Microbial sensors for determination of aromatics and their chloroderivatives II. Determination of chlorinated phenols using a Rhodococcus-containing biosensor. Appl. Microbiol. Biotechnol. 38: 556–559.

    Article  CAS  Google Scholar 

  • Rogers, K.R. (1995) Biosensors for environmental applications. Biosens. Bioelectron. 10: 533–541.

    Article  CAS  Google Scholar 

  • Ruzicka, J. and Hansen, E.H. (1988) Flow injection analysis, 2nd edn. John Wiley & Sons, New York, N.Y.

    Google Scholar 

  • Scheper, T., Brandes, W, Maschke, H., Plötz, E. and Müller, C. (1993) Two FIA-based biosensor systems studies for bioprocess control. J. Biotechnol. 31: 345–356.

    Article  PubMed  CAS  Google Scholar 

  • Slama, M., Zaborosch, C. and Spener, F. (1995) Microbial sensor for rapid estimation of the biochemical oxygen demand (BOD) in presence of heavy metal ions. In: R.D. Wilken, A. Knöchel and U. Förstner (eds.): Int. Conference Heavy Metals in the Environment, Vol. 2. CEP Consultants Ltd., Edinburgh, U.K., pp 171–174.

    Google Scholar 

  • Steinkuhl, R., Hinkers, H., Dumschat, C., Knoll, M. and Camman, K. (1994) Glucose sensor in containment technology. Horm. Metab. Res. 26: 531–533.

    Article  PubMed  CAS  Google Scholar 

  • Steinkuhl, R., Dumschat, C., Sundermeier, C., Renneberg, R., Cammann, K. and Knoll, M. (1996) Micromachined glucose sensor. Biosens. Bioelectron. 11: 187–190.

    Article  CAS  Google Scholar 

  • Tang, H.T., Lunte, C.E., Halsall, H.B. and Heineman, W.R. (1988) p-Aminophenyl phosphate: an improved substrate for electrochemical enzyme immunoassay. Anal. Chim. Acta 214: 187–195.

    Google Scholar 

  • Vorlop, K.D., Muscat, A. and Beyersdorf, J. (1992) Entrapment of microbial cells within polyurethane hydrogel beads with the advantage of low toxicity. Biotechnol. Tech. 6: 483–488.

    Article  CAS  Google Scholar 

  • VROM/NL (1988) Leidraad Bodensanering, Deel II, Technisch-Inhondelijk Deel. In deutscher Übersetzung ( 1989 ): Leitfaden Bodensanierung, BMU, Teil 2.

    Google Scholar 

  • Wehmeyer, K.R., Halsall, H.B. and Heineman, W.R. (1985) Heterogeneous enzyme immunoassay with electrochemical detection: competitive and “sandwich”-type immunoassays. Clin. Chem. 31: 1546–1549.

    PubMed  CAS  Google Scholar 

  • White, S.E, Turner, A.P.E., Bilitewski, U., Schmid, R.D. and Bradley, J. (1994) Lactate, glutamate and glutamine biosensors based on rhodinised carbon electrodes. Anal. Chim. Acta 295: 243–251.

    Article  CAS  Google Scholar 

  • White, S.F., Turner, A.P.F., Bilitewski, U., Bradley, J. and Schmid, R.D. (1995) On-line monitoring of glucose, glutamate and glutamine during mammalian cell cultivations. Biosens. Bio-electron. 10: 543–551.

    CAS  Google Scholar 

  • Wilmer, M., Renneberg, R. and Spener, E (1996a) Rapid enzyme-immunoassay for the detection of 2,4-dichlorophenoxyacetic acid (2,4-D) in water using monoclonal antibodies. Vom Wasser 86: 83–93.

    CAS  Google Scholar 

  • Wilmer, M., Trau, D., Renneberg, R. and Spener, E (1996b) Amperometric immunosensor for the detection of 2,4-dichlorophenoxyacetic acid (2,4-D) in water. Anal. Lett.,in press.

    Google Scholar 

  • Xie, B., Mecklenburg, M., Danielsson, B.,.Öhman, O., Norlin, P. and Winquist, E (1995) Development of an integrated thermal biosensor for the simultaneous determination of multiple analytes. Analyst 120: 155–160.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Chemo- und Biosensorik, D-48149, Münster, Germany

    F. Spener, M. Meusel & C. Siegmann-Thoss

Authors
  1. F. Spener
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Meusel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Siegmann-Thoss
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institut für Biochemie und Molekulare Physiologie, c/o Max-Delbrück-Centrum für Molekulare Medizin, Robert-Rössle-Strasse 10, D-13122, Berlin, Germany

    F. W. Scheller

  2. Physikalisch-Technische Bundesanstalt, Abbestrasse 2-12, D-10587, Berlin, Germany

    F. Schubert

  3. c/o Centrum für Hochschulentwicklung, PO Box 105, D-33311, Gütersloh, Germany

    J. Fedrowitz

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Birkhäuser Verlag Basel/Switzerland

About this chapter

Cite this chapter

Spener, F., Meusel, M., Siegmann-Thoss, C. (1997). Biosensors based on flow-through systems. In: Scheller, F.W., Schubert, F., Fedrowitz, J. (eds) Frontiers in Biosensorics II. EXS, vol 81. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-9045-8_3

Download citation

  • DOI: https://doi.org/10.1007/978-3-0348-9045-8_3

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-0348-9884-3

  • Online ISBN: 978-3-0348-9045-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation