Summary
Molecular recognition by non covalent interactions is of key importance not only in fundamental biochemistry, but also in affinity-based analytics. In typical affinity assays labelled compounds are used for detection of assay response. In contrast, the label-free detection of molecular interaction allows a more straigthforward approach to binding detection, simplified test schemes, and additional information about kinetical characteristics of the interaction. Optical techniques are particulary useful in direct affinity detection. One approach, based on white light interferometry is discussed in detail. This technique monitors the change in thickness of surface-bound layers of biological material by white light interference. Applications are given from quantitative detection of high molecular weight analytes, detection of low molecular weight analytes in a competitive test scheme, direct detection of low molecular weight analytes with immobilised receptors, investigation of interaction kinetics, and thermodynamic analysis of binding equilibrium. Finally, an outlook with respect to low-cost bioanalytical systems and high throughput screening applications is given, comparing various transducers and demonstrating advantages of label-free detection.
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© 1997 Birkhäuser Verlag Basel/Switzerland
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Brecht, A., Gauglitz, G. (1997). Reflectometric interference spectroscopy for direct affinity sensing. In: Scheller, F.W., Schubert, F., Fedrowitz, J. (eds) Frontiers in Biosensorics II. EXS, vol 81. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-9045-8_1
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DOI: https://doi.org/10.1007/978-3-0348-9045-8_1
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