Skip to main content
SpringerLink
Log in

Screening and characterization of new enzymes for biosensing and analytics

  • Chapter
  • First Online:
Frontiers in Biosensorics I

Part of the book series: Experientia Supplementum ((EXS,volume 80))

  • 169 Accesses

Summary

The development of new or improved analytical methods requires new enzymes. Screening techniques utilizing enrichment cultures and rapid assay methods supported by automated or miniaturized methods are useful tools to detect new enzyme producers. Notably, oxidoreductases are well suited for analytical purposes. The NAD(P)- and oxygen-independent quinoprotein dehydrogenases with a covalently bound redox cofactor can be used advantageously for the development of biosensors. Examples are given of selective enrichment methods used in screening for useful enzyme-producing microorganisms. Enrichment under chemostatic conditions proved to be successful because enzymes with a remarkably high affinity against the analyte could be obtained. This is demonstrated by the screening of a trimethylamine-converting enzyme. The frequently observed high selectivity of these enzymes against the substrate is demonstrated in a few examples. In exploitation of these new oxidoreductases, new analytical methods were developed which are useful for the detection and during monitoring of phenylketonuria (PKU) or maple syrup urine disease (MSUD).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Reference

  • Cheetham, P.S.J. (1987) Screening for novel biocatalysts. Enz. Microb. Technol. 9: 194–213.

    Article  CAS  Google Scholar 

  • D’Costa, E.J., Higgins, I.J. and Turner, A.P.F. (1986) Qunioprotein glucose dehydrogenase and ist application in an amperometric glucose sens. Biosensors 2: 71–87.

    Article  Google Scholar 

  • Duine, J.A. (1991) Quinoproteins: enzymes containing the quinoid cofactor pyrroloquinoline quinone, topaquinone or tryptophan-tryptophan quinone. Eur. J. Biochem. 200: 271–284.

    Article  CAS  Google Scholar 

  • Duine, J.A. and Jongejan, J.A. (1989) Quinoproteins, enzymes with pyrrolo-quinoline quinone as cofactor. Ann. Rev. Biochem. 58: 403–426.

    Article  CAS  Google Scholar 

  • Duine, J.A., Frank, Jzn.J. and Verwiel, P.E.J. (1980) Structure and activity of the prosthetic group of methanol dehydrogenase. Eur. J. Biochem. 108: 187–192.

    Article  CAS  Google Scholar 

  • Elander, R.P. (1987) Microbial screening, selection and strian improvement. In: J. Bu’Lock and B. Kristiansen (eds): Basic Biotechnology. Academic Press, London, pp 217–251.

    Google Scholar 

  • Goodhue, C.T. (1982) The methodoly of microbial transformation of organic compounds. In: J.P Rosazza (ed): Microbial transformations ofbioactive compounds. Vol I. CRC Press, Boca Raton, Florida, pp 9–44.

    Google Scholar 

  • Guthrie, R. and Susi, A. (1963) Pediatrics 32: 338–343.

    CAS  PubMed  Google Scholar 

  • Hummel, W. and Kula, M.-R. (1989) Simple method for small-scale disruption of bacteria and yeasts. J. Microbiol. Meth. 9: 201–209.

    Article  Google Scholar 

  • Hummel, W., Weiss, N. and Kula, M.-R. (1984) Isolation and characterization of a bacterium possessing L-phenylalanine dehydrogenase activity. Arch. Microbiol. 137: 47–52.

    Article  CAS  Google Scholar 

  • Hummel, W., Schütte, H. and Kula, M.-R. (1985) D-2-Hydroxyisocaproate dehydrogenase from Lactobacillus casei - A new enzyme suitable for the stereospecific reduction of 2-ketocar- boxylic acids. Appl. Microbiol. Biotechnol. 21: 7–15.

    CAS  Google Scholar 

  • Hummel, W., Schmidt, E., Wandrey, C. and Kula, M.-R. (1986) L-Phenylalanine dehydrogenase from Brevibacteri um sp. for production of L-phenylalanine by reductive amination of phenyl- pyruvate. Appl. Microbiol. Biotechnol. 25: 175–185.

    Article  CAS  Google Scholar 

  • Hummel, W, Schmidt, E., Schütte, H. and Kula, M.-R. ( 1987 a) Isolation of microorganisms containing high levels of phenylalanine dehydrogenase. Proc. Biochemical Engineering, Fischer Verlag, Stuttgart, pp 392–395.

    Google Scholar 

  • Hummel, W., Schütte, H., Schmidt, E., Wandrey, C. and Kula, M.-R. (1987b) Isolation of L-phenylalanine dehydrogenase from Rhodococcus sp. M4 and its application for the production of L-phenylalanine. Appl. Microbiol. Biotechnol. 26: 409–416.

    Article  CAS  Google Scholar 

  • Hummel, W., Schütte, H. and Kula, M.-R. (1988 a) D-(-)-Mandelic acid dehydrogenase from Lactobacillus curvatus. Appl. Microbiol. Biotechnol. 28: 433–439.

    Article  CAS  Google Scholar 

  • Hummel, W., Schütte, H. and Kula, M.-R. (1988b) Enzymatic determination of L-phenylalanine and phenylpyruvate with L-phenylalanine dehydrogenase. Anal. Biochem. 170:397–401.

    Article  CAS  Google Scholar 

  • Hummel, W., Tauschensky, S., Spohn, U., Wendel, U. and Langenbeck, U. (1989) Towards Home-Monitoring and screening of phenylketonuria by biosensors. Studies on flow-injection analysis. In: R.D. Schmid and F. Scheller (eds): Biosensors: Applications in Medicine, Environmental protection and process control. GBF-Monographs 13. VCH Verlagsgesellschaft, Weinheini, pp 313–318.

    Google Scholar 

  • Hummel, W., Wendel, U. and Sting, S. (1992) Biochemical characterization of a highly specific trimethylamine dehydrogenase suited for the application in biosensors. In: F. Scheller and R.D. Schmid (eds) Biosensors: Fundamentals, Technologies and Applications. GBF Monographs 17. VCH Publishers, Weinheim, pp 381–384.

    Google Scholar 

  • Morin, A., Hummel, W. and Kula, M.-R. (1986) Rapid detection of microbial hydantoinase on solid medium. Biotechnol. Lett. 8: 571–576.

    Article  CAS  Google Scholar 

  • Morin, A., Hummel, W. and Kula, M.-R. (1987) Enrichment and selection of hydantoinase producing microorganism. J. Gen. Microbiol. 133: 1201–1207.

    CAS  Google Scholar 

  • Schadewaldt, R, Hummel, W., Trautvetter, U. and Wendel, U. (1989) A convenient enzymatic method for the determination of 4-methyl-2-oxopentanoate in plasma: Comparison with High Performance Liquid Chromatographic analysis. Clin. Chim. Acta 183: 171–182.

    Article  CAS  Google Scholar 

  • Schadewaldt, P., Hummel, W, Wendel, U. and Adelmeyer, F. (1995) Enzymatic method for determination of branched-chain amino acid aminotransferase activity. Anal. Biochem. 230: 199–204.

    Article  CAS  Google Scholar 

  • Schütte, H., Hummel, W. and Kula, M.-R. (1984) L-Hydroxyisocaproate dehydrogenase - a new enzyme from Lactobacillus confusus for the stereospecific reduction of 2-ketocarboxy- lic acids. Appl. Microbiol. Biotechnol. 19: 167–176.

    Article  Google Scholar 

  • Schütte, H., Hummel, W. and Kula, M.-R. (1985) Improved enzyme screening by automated fast protein liquid chromatography. Anal. Biochem. 151: 547–553.

    Article  Google Scholar 

  • Wendel, U., Hummel, W. and Langenbeck, U. (1989) Monitoring of Phenylketonuria: A colorimetric method for the determination of plasma phenylalanine using L-phenylalanine dehydrogenase. Anal. Biochem. 180: 91–94.

    Article  CAS  Google Scholar 

  • Wendel, U., Koppelkamm, M, Hummel, W., Sander, J. and Langenbeck, U. (1990 a) A new approch to the newborn screening for hyperphenylalaninemias: Use of L-phenylalanine dehydrogenase and microtiter plates. Clin. Chim. Acta 192: 165–170.

    CAS  Google Scholar 

  • Wendel, U., Özalp, I., Langenbeck, U. and Hummel, W. (1990b) Phenylketonuria in Turkey: Experience with an enzymatic colorimetric test for measurement of serum phenylalanine. J. InheritedMetab. Disease 13: 295–297.

    Article  CAS  Google Scholar 

  • Wendel, U., Koppelkamm, M. and Hummel, W. (1991) Enzymatic phenylalanine estimation for the management of patients with phenylketonuria. Clin. Chim. Acta 201: 95–98.

    Article  CAS  Google Scholar 

  • Yamazaki, Y., Hummel, W. and Kula, M.-R. (1987) Ein neues Verfahren zum direkten Nachweis mikrobieller Aminoacylaseaktivität auf Agarplatten. Z. Naturforsch. 42c: 1082–1088.

    Article  CAS  Google Scholar 

  • Yamazaki, Y. and Kula, M.-R. (1987) Entwicklung neuer Plattentests zum Nachweis mikrobieller Hydrolysen von Estern und Oxidation von 2-Hydroxycarbonsäuren. Z Naturforsch. 42c: 1187–1192.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf, Forschungszentrum Jülich, Jülich, D-52404, Germany

    W. Hummel

Authors
  1. W. Hummel
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institut für Biochemie und Molekulare Physiologie c/o Max-Delbrück-Center für Molekulare Medizin, Robert-Rössle-Strasse 10, D-13 122, Berlin, Germany

    F. W. Scheller

  2. Physikalisch-Technische Bundesanstalt, Abbestrasse 2-12, D-10587, Berlin, Germany

    F. Schubert

  3. Centrum für Hochschulentwicklung, PO Box 105, D-33311, Gütersloh, Germany

    J. Fedrowitz

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Birkhäuser Verlag

About this chapter

Cite this chapter

Hummel, W. (1997). Screening and characterization of new enzymes for biosensing and analytics. In: Scheller, F.W., Schubert, F., Fedrowitz, J. (eds) Frontiers in Biosensorics I. Experientia Supplementum, vol 80. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-9043-4_4

Download citation

  • DOI: https://doi.org/10.1007/978-3-0348-9043-4_4

  • Published:

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-9883-6

  • Online ISBN: 978-3-0348-9043-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation