Skip to main content
SpringerLink
Log in

Förster energy transfer in ultrathin polymer layers as a basis for biosensors

  • Chapter
  • First Online:
Frontiers in Biosensorics I

Part of the book series: Experientia Supplementum ((EXS,volume 80))

  • 160 Accesses

Summary

A method of detecting the binding of analyte molecules to biospecific receptors, like antibodies, is described. Förster energy transfer is used in connection with monomolecular organic films. The films are built up from pre-polymerized materials using the Langmuir-Blodgett or self-assembly techniques. Fluorescent dyes (as energy transfer donors) as well as reactive groups for covalent immobilization of protein receptors are integrated into the polymers. Several different methods for immobilizing biomolecules are described, including the use of protein A and the biotin/streptavidin couple. The studies suggest that the combination of Förster transfer and ultrathin organic films can be used for the construction of biosensors working either by displacement or by competitive assays. The mannose/concanavalin A, digoxin/antibody, and mouse immunoglobulin G/antibody systems are investigated. Further, a simplified meter optimized for measuring the fluorescence ratio at two wavelengths is described.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Ahlers, M., Müller, W., Reichert, A., Ringsdorf, H. and Venzmer, J. (1990) Spezifische Wechselwirkung von Proteinen mit funktionellen Lipidmonoschichten—Wege zur Simulation von Biomembranprozessen. Angew. Chem. 102:1310–1327; Angew. Chem. Int. Ed. Engl. 29: 1269–1285.

    Article  CAS  Google Scholar 

  • Decher, G. (1993) Supramolekulare Chemie: Ultradünne Schichten aus Polyelektrolyten. Nachr. Chem. Tech. Lab. 41: 793–800.

    Article  CAS  Google Scholar 

  • Decher, G. and Hong, J.D. (1991) Buildup of Ultrathin Multilayer Films by a Self- Assembly Process: II. Consecutive Adsorption of Anionic and Cationic Bipolar Amphiphiles and Polyelectrolytes on Charged Surfaces. Ber. Bunsenges. Phys. Chem. 95: 1430–1434.

    Article  CAS  Google Scholar 

  • Diederich, A. (1996) Dissertation, University of Mainz, Strukturelle und funktionelle Untersuchungen von Proteinmomo- und -multischichten an technischen Grenzflächen.

    Google Scholar 

  • Embs, F., Funhoff, D., Laschewski, A., Licht, U., Ohst, H., Prass, W, Ringsdorf, H., Wegner, G. and Wehrmann, R. (1991) Preformed Polymers for Langmuir-Blodgett Films—Molecular Concepts. Adv. Mater. 3: 25–31.

    Article  CAS  Google Scholar 

  • Embs, F.W., Winter, H.H. and Wegner, G. (1993) Langmuir-Blodgett multilayer assembly by a continuous process using a steadily flowing subphase. Langmuir 9: 161 8–1621.

    Google Scholar 

  • Fromherz, P. and Reinbold, G. (1988) Energy transfer between fluorescent dyes spaced by multilayers of cadmium salts of fatty acids. Thin Solid Films 160: 347–353.

    Article  CAS  Google Scholar 

  • Fuchs, H., Ohst, H. and Prass, W. (1991) Ultrathin Organic Films: Molecular Architectures for Advanced Optical, Electronic and Bio-Related Systems. Adv. Mater. 3: 10–18.

    Article  CAS  Google Scholar 

  • Gabor, G., Chadha, S. and Walt, D.R. (1995) Sensitivity enhancement of fluorescent pH indicators using pH-dependent energy transfer. Anal. Chim. Acta 313: 131–137.

    Article  CAS  Google Scholar 

  • Heiliger, L. and Siegmund, H.-U. (1993) Beschichtete Träger, Verfahren zu ihrer Herstellung und ihre Verwendung zur Immobilisierung von Biomolekülen an Oberflächen von Fest¬körpern. German Patent Application (Bayer AG) DE 43 19 037.

    Google Scholar 

  • Hugl, H., Kuckert, E., Möbius, D., Ohst, H., Rolf, M., Rosenkranz, HJ., Schopper, H.C., Siegmund, H.-U., Sommer, K. and Wehrmann, R., Optischer Biosensor. German Patent Applications (Bayer AG) DE 39 38 598 (1989), DE 40 13 713 (1990).

    Google Scholar 

  • Kauffmann, F., Hoffmann, B„, Erbach, R., Heiliger, L., Siegmund, H.-U. and Völker, M. (1994) Ca2+ sensor with amphiphilic Langmuir-Blodgett membranes. Sensors Actuators B 18–19: 60–64.

    Article  Google Scholar 

  • Kuhn, H. (1970) Classical aspects of energy transfer in molecular systems. J. Phys. Chem. 53: 101–108.

    Article  CAS  Google Scholar 

  • Kuhn, H.,Möbius, D. and Bücher, H. (1972) Spectroscopy of Monolayer Assemblies. In: A. Weissberger and B. Rossiter (eds): Physical Methods of Chemistry, Vol. 1, Part 3B. John Wiley, New York, pp 577–702.

    Google Scholar 

  • Lvov, Y., Decher, G. and Möhwald, H. (1993) Assembly, Structural Characterization, and Thermal Behavior of Layer-by-Layer Deposited Ultrathin Films of Poly(vinyl sulfate) and Poly (ally lamine). Langmuir 9: 481–486.

    Article  CAS  Google Scholar 

  • Müller, H., Ringsdorf, H., Rump, E., Wildburg, G., Zhang, X., Angermaier, L., Knoll, W., Liley, M. and Spinke, J. (1993) Attempts to Mimic Docking Processes of the Immune System: Recognition-Induced Formation of Protein Multilayers. Science 262: 1706–1708.

    Article  Google Scholar 

  • Scouten, W.H. (1987) A Survey of Enzyme Coupling Techniques. In: K. Mosbach (ed): Methods in Enzymology, Vol. 135. Academic Press, New York, pp 30–65.

    Google Scholar 

  • Siegmund, H.-U., Becker, A. and Möbius, D. (1991) Characterization and Optimization of Lang- muir-Blodgett Films Containing Polymer-Bound Fluorophores. Adv. Mater. 3: 605–608.

    Article  CAS  Google Scholar 

  • Siegmund, H.-U., Heiliger, L., van Lent, B. and Becker, A. (1992a) Optischer Festphasenbio- sensor auf Basis fluoreszenzfarbstoffmarkierter polyionischer Schichten. German Patent Application (Bayer AG) DE 42 08 645.

    Google Scholar 

  • Siegmund, H.-U., Becker, A., Ohst, H. and Sommer, K. (1992b) An optical biosensor principle based on fluorescence energy transfer. Thin Solid Films 210 /211: 480–483.

    Article  Google Scholar 

  • Siegmund, H.-U. and Becker, A. (1993) A new way of biosensing using fluorescence energy transfer and Langmuir-Blodgett films. Sensors Actuators 5 11: 103–108.

    Article  Google Scholar 

  • Sommer, R.G., Belchak, T.L., Bloczynski, M.L., Boguslawski, S.J., Clay, D.L., Corey, P.F., Folz, M.M., Fredrickson, R.A., Haimo, B.L., Johnson, R.D., Marfurt, K.L., Runzheimer, H.-V and Morris, D.L. (1990) A Unitzed Enzyme-Labeled Immunometric Digoxin Assay Suitable for Rapid Testing. Clin. Chem. 36: 201–206.

    Article  CAS  Google Scholar 

  • Stryer, L. (1978) Fluorescence energy transfer as a spectroscopic ruler. Annu. Rev. Biochem. 47: 819–846.

    Article  CAS  Google Scholar 

  • Thompson, R.B. and Patchman, M.W. (1995) Lifetime-Based Fluorescence Energy Transfer Biosensing of Zinc. Anal. Biochem. 227: 123–128.

    Article  CAS  Google Scholar 

  • Tippmann-Krayer, P., Riegler, H., Paudler, M., Möhwald, H., Siegmund, H.-U., Eickmans, J., Scheunemann, U., Licht, U. and Schrepp, W. (1991) Thermostability of Polymeric Langmuir- Blodgett Films. Adv. Mater. 3: 46–51.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Zentrale Forschung und Entwicklung Uerdingen, Bayer AG, Krefeld, D-47812, Germany

    M. Völker

  2. Diagnostics Division, Bayer Corp., Elkhart, IN, 46515, USA

    H.-U. Siegmund

Authors
  1. M. Völker
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H.-U. Siegmund
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institut für Biochemie und Molekulare Physiologie c/o Max-Delbrück-Center für Molekulare Medizin, Robert-Rössle-Strasse 10, D-13 122, Berlin, Germany

    F. W. Scheller

  2. Physikalisch-Technische Bundesanstalt, Abbestrasse 2-12, D-10587, Berlin, Germany

    F. Schubert

  3. Centrum für Hochschulentwicklung, PO Box 105, D-33311, Gütersloh, Germany

    J. Fedrowitz

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Birkhäuser Verlag

About this chapter

Cite this chapter

Völker, M., Siegmund, HU. (1997). Förster energy transfer in ultrathin polymer layers as a basis for biosensors. In: Scheller, F.W., Schubert, F., Fedrowitz, J. (eds) Frontiers in Biosensorics I. Experientia Supplementum, vol 80. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-9043-4_12

Download citation

  • DOI: https://doi.org/10.1007/978-3-0348-9043-4_12

  • Published:

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-9883-6

  • Online ISBN: 978-3-0348-9043-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation