Summary
Previous starch gel electrophoretic studies on transferrin have indicated considerable polymorphism in brown trout populations with diagnostic alleles for Atlantic salmon and various brown trout subspecies and races. Transferrin polymorphism is of interest for studies and monitoring of natural or anthropogenically induced hybridization between Atlantic salmon and brown trout or between several brown trout subspecies and races because only small samples of blood (100 μl) are needed which may be obtained without killing the fish. We present a fast and simple method for the separation of transferrin alleles. We recommend cellulose acetate electrophoresis which has several advantages as compared to previous methods of separation and which is suitable for routine analysis of large samples within short time.
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© 1996 Birkhäuser Verlag Basel/Switzerland
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Largiadèr, C.R., Scholl, A. (1996). Cellulose acetate electrophoresis for screening transferrin polymorphism in brown trout (Salmo trutta L.) populations. In: Kirchhofer, A., Hefti, D. (eds) Conservation of Endangered Freshwater Fish in Europe. ALS Advances in Life Sciences. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-9014-4_19
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DOI: https://doi.org/10.1007/978-3-0348-9014-4_19
Publisher Name: Birkhäuser Basel
Print ISBN: 978-3-0348-9867-6
Online ISBN: 978-3-0348-9014-4
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