Summary
Unlike neurosecretory release organs of vertebrates, equivalent sites in the body of invertebrates with an open circulatory system, are well suited for scanning electron microscopy (SEM). The present paper describes such a SEM study of the principal neurohaemal organ of insects, i.e., the corpus cardiacum. Besides a classical fixation protocol using glutaraldehyde, two additional — uncommon — methods of tissue preparation were examined, one of which employed Bouin-Hollande’s 10% sublimate as fixative. The various preparations all yielded different scanning electron micrographs. The images observed are correlated with previously described ultrastructural data on various types of (invertebrate) neurosecretory release profiles and with the ”fixing” strength (speed) of the chemical solutions used.
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© 1996 Birkhäuser Verlag Basel/Switzerland
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Verhaert, P.D., De Loof, A.J. (1996). Scanning electron microscopy of an active neurohaemal area, the cockroach (Periplaneta americana) corpora cardiaca: looking at neurosecretion from an unprecedented viewpoint. In: Krisch, B., Mentlein, R. (eds) The Peptidergic Neuron. Advances in Life Sciences. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-9010-6_8
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DOI: https://doi.org/10.1007/978-3-0348-9010-6_8
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