Abstract
As more complete bacterial genome sequences become available, the need for broad, genomic-scale analytical methods becomes increasingly acute. Moreover, with a sizable portion of microbial genomes encoding proteins of unknown function, tools that suggest gene function or elucidate relationships among genes are crucial for any genomic-scale evaluation. While genomics tools may be central to a basic scientist’s interests in a model organism, they will also be critical to the applied scientist’s examination of a pathogen for targets for therapeutic intervention.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Link A, Phillips D and Church G (1997) Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization. J Bacteriol 179: 6228–6237
Posfai G, Kolisnychenko V, Bereczki Z et al. (1999) Markerless gene replacement in Escherichia coli stimulated by a double-strand break in the chromosome. Nucleic Acids Res 27: 4409–4415
Datsenko K and Wanner B (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA 97: 6640–6645
Karberg M, Guo H, Zhong J, et al. (2001) Group II introns as controllable gene targeting vectors for genetic manipulation of bacteria. Nature Biotech 19: 1162–1167
Akerley BJ, Rubin EJ, Camilli A, et al. (1998) Systematic identification of essential genes by in vitro mariner muta-genesis. Proc Natl Acad Sci USA 95: 8927–8932
Reich KA, Chovan L, Hessler P (1999) Genome scanning in Haemophilus influenza for identification of essential genes. J Bacteriol 181: 4961–4968
Hare R, Walker S, Dorman T, et al. (2001) Genetic footprinting in bacteria. J Bacteriology 183: 1694–1706
Smith V, Botstein D, Brown PO (1995) Genetic footprinting: a genomic strategy for determining a gene’s function given its sequence. Proc Natl Acad Sci USA 92: 6479–6483
Smith V, Chou KN, Lashkari D, et al. (1996) Functional analysis of the genes of yeast chromosome V by genetic foot-printing. Science 274: 2069–2074
Kleckner N, Bender J, Gottesman S (1991) Uses of transposons with emphasis on Tn10. In: JH Miller (ed): Methods in enzymology: Bacterial genetic systems. Academic Press, New York, 139–180
Davis RW, Botstein D, Roth JR (1980) A manual for genetic engineering: Advanced bacterial genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Jensen KF (1993) The Escherichia coli K12 “wild types” W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. J Bacteriol 175: 3401–3407
Metcalf WW, Wanner BL (1993) Mutational analysis of an Escherichia coli fourteen-gene operon for phosphonate degradation, using TnphoA elements. J Bacteriol 175: 3430–3432
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Birkhäuser Verlag Basel/Switzerland
About this chapter
Cite this chapter
Walker, S.S., Houseweart, C., Kenney, T.J. (2003). Genetic Footprinting for Bacterial Functional Genomics. In: Blot, M. (eds) Prokaryotic Genomics. Methods and Tools in Biosciences and Medicine. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-8963-6_8
Download citation
DOI: https://doi.org/10.1007/978-3-0348-8963-6_8
Publisher Name: Birkhäuser Basel
Print ISBN: 978-3-7643-6596-7
Online ISBN: 978-3-0348-8963-6
eBook Packages: Springer Book Archive