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Prokaryotic Genomics pp 65–89Cite as

Use of Conditional-replication, Integration, and Modular CRIM Plasmids to Make Single-copy lacZ Fusions

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Part of the book series: Methods and Tools in Biosciences and Medicine ((MTBM))

Abstract

Ever since e fusion of the lac operon to a foreign promoter (for a purine biosynthetic gene) was shown to Subject the lacY gene to exogenous control (by purines [1]), lac reporter fusions have been proven to be invaluable to innumerable studies of gene Structure, gene regulation, protein localization, protein folding, protein-protein interactions, and other aspects of cell biology [2]. Consequently, many protocols and vectors have been developed over the years for the construction of lac fusions, not only in E. coli and other bacteria but also in many other cell types [3–5].

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Authors
  1. Lu Zhou
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  2. Soo-Ki Kim
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  3. Larisa Avramova
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  4. Kirill A. Datsenko
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  5. Barry L. Wanner
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Editors and Affiliations

  1. Université Joseph Fourier, 460 rue de la piscine, 38400, St Martin d’Hères, France

    Michel Blot

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Zhou, L., Kim, SK., Avramova, L., Datsenko, K.A., Wanner, B.L. (2003). Use of Conditional-replication, Integration, and Modular CRIM Plasmids to Make Single-copy lacZ Fusions. In: Blot, M. (eds) Prokaryotic Genomics. Methods and Tools in Biosciences and Medicine. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-8963-6_7

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  • DOI: https://doi.org/10.1007/978-3-0348-8963-6_7

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-7643-6596-7

  • Online ISBN: 978-3-0348-8963-6

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