Abstract
Gene amplification is a powerful technique with a number of applications. Multicopy suppression, whereby the phenotype caused by loss of one gene product is corrected by increasing another, can provide information on possible protein-protein interactions in the cell [1]. Increased concentrations of particular enzymes can reveal weak activities and possible “underground” reactions with alternative substrates [2]. Amplification of a specific DNA binding site, coupled with expression of the corresponding DNA-binding protein carrying a fluorescence label, has permitted subcellular labelling of specific chromosomal regions [3, 4].
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© 2003 Birkhäuser Verlag Basel/Switzerland
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D’Ari, R., Vinella, D. (2003). How to Amplify Easily, on the Bacterial Chromosome, a Desired DNA Sequence. In: Blot, M. (eds) Prokaryotic Genomics. Methods and Tools in Biosciences and Medicine. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-8963-6_5
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DOI: https://doi.org/10.1007/978-3-0348-8963-6_5
Publisher Name: Birkhäuser Basel
Print ISBN: 978-3-7643-6596-7
Online ISBN: 978-3-0348-8963-6
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