Skip to main content

Two-hybrid Assay in Escherichia coli K12

  • Chapter
Prokaryotic Genomics

Abstract

Many manifestations of living organisms are the result of protein activities that need the formation of the protein multimers themselves or of macromolecular complexes with other proteins. Numerous in vivo and in vitro approaches have been developed to facilitate the study of protein interactions [1–3].

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Fields S, Song O (1989) A novel genetic system to detect protein-protein interactions. Nature 340: 245–246

    Article  CAS  Google Scholar 

  2. Hanes J, Pluckthun A (1999) In vitro selection methods for screening of peptide and protein libraries. Curr Top Microbiol Immunol 243: 107–122

    Article  CAS  Google Scholar 

  3. Vidal M, Legrain P (1999) Yeast forward and reverse n-hybrid systems. Nucleic Acids Res 27: 919–929

    Article  CAS  Google Scholar 

  4. Dove SL, Joung JK, Hochschild A (1997) Activation of prokaryotic transcription through arbitrary protein-protein contacts. Nature 386: 627–630

    Article  CAS  Google Scholar 

  5. Karimova G, Pidoux J, Ullmann A, Ladant D (1998) A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proc Natl Acad 11 Sci USA 95: 5752–5756

    Article  CAS  Google Scholar 

  6. Kornacker MG, Remsburg B, Menzel R (1998) Gene activation by the AraC protein can be inhibited by DNA looping between AraC and a LexA repressor that interacts with AraC: possible applications as a two-hybrid system. Mol Microbiol 30: 615–624

    Article  CAS  Google Scholar 

  7. Karimova G, Ullmann A, Ladant D (2000) A bacterial two-hybrid system that exploits a cAMP signaling cascade in Escherichia coli. Methods Enzymol 328: 59–73

    Article  CAS  Google Scholar 

  8. Hu JC, O’Shea EK, Kim P, Sauer RT (1990) Sequence requirements for coiled-coils: analysis with l repressor-GCN4 Leucine zipper fusions. Science 250: 1400–1403

    Article  CAS  Google Scholar 

  9. Longo F, Marchetti MA, Castagnoli L, Battaglia PA, Gigliani F (1995) A novel approach to protein-protein interaction: complex formation between the p53 tumor suppressor and the HIV Tat proteins. Biochem Bilphys Res Commun 206: 326–334

    Article  CAS  Google Scholar 

  10. Di Lallo G, Ghelardini P, Paolozzi L (1999) Two-hybrid assay: construction of an Escherichia coli system to quantify homodimerization ability in vivo. Microbiol 145: 1485–1490

    Article  Google Scholar 

  11. Di Lallo G, Castagnoli L, Ghelardini P, Paolozzi L (2001) A two-hybrid system based on chimeric operator recognition for studying protein homo/heterodimerization in Escherichia coli. Microbiol 147: 651–1656

    Google Scholar 

  12. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY

    Google Scholar 

  13. Dente L, Cesareni G, Cortese R (1983) Pembl: a new family of single stranded plasmids. Nucleic Acid Res 11: 1645–1655

    Article  CAS  Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2003 Birkhäuser Verlag Basel/Switzerland

About this chapter

Cite this chapter

Di Lallo, G., Ghelardini, P., Paolozzi, L. (2003). Two-hybrid Assay in Escherichia coli K12. In: Blot, M. (eds) Prokaryotic Genomics. Methods and Tools in Biosciences and Medicine. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-8963-6_15

Download citation

  • DOI: https://doi.org/10.1007/978-3-0348-8963-6_15

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-7643-6596-7

  • Online ISBN: 978-3-0348-8963-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics