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Tight junctions and adherens junctions in endothelial Cells: structure and regulation

  • Chapter
Vascular Adhesion Molecules and Inflammation

Part of the book series: Progress in Inflammation Research ((PIR))

Abstract

Endothelial cells lining the vasculature provide a crucial interface between plasma and tissue environments, separating the solute, macromolecular and cellular composition of the plasma from that of the interstitial fluid [1–3]. This chapter will focus on the role of endothelial cell-cell adhesion as relates to this function. In particular, the tight junction plays the most important role in the separation of plasma and tissue environments. The tight junction also plays a crucial role in the establishment and maintenance of cellular polarity, the separation of the plasma membrane into apical and basolateral compartments, important for the vectorial transport of essential nutrients and factors across endothelia with especially well developed tight junctions [4]. An example of such a differentiated endothelium is the blood-brain barrier, where tight junctions are so well developed that even ionic permeability is severely limited [5]. In epithelial cells [6] and endothelial cells [3, 7, 8], the establishment of tight junctions is dependent on the prior formation of intercellular adherens junctions. For this reason, the adherens junction will also be discussed. Both tight and adherens junctions are composed of transmembrane proteins with associated cytoplasmic components. Also, both junctions are linked to the actin-based cytoskeleton which in itself is subject to complex regulation.

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References

  1. Risau W (1995) Differentiation of endothelium. FASEB J 9: 926–933

    PubMed  CAS  Google Scholar 

  2. Rubin LL (1992) Endothelial cells: adhesion and tight junctions. Curr Opin Cell Biol 4: 830–833

    Article  PubMed  CAS  Google Scholar 

  3. Dejana E, Corada M, Lampugnani MG (1995) Endothelial cell-to-cell junctions. FASEB J 9: 910–918

    PubMed  CAS  Google Scholar 

  4. Nelson WJ (1992) Regulation of cell surface polarity from bacteria to mammals. Science 258: 948–955

    Article  PubMed  CAS  Google Scholar 

  5. Staddon JM, Rubin LL (1996) Cell adhesion, cell junctions and the blood-brain barrier. Curr Opin Neurobiol 6: 622–627

    Article  PubMed  CAS  Google Scholar 

  6. Gumbiner BM, Simons K (1986) A functional assay for proteins involved in establishing an epithelial occluding barrier: identification of a uvomorulin-like polypeptide. J Cell Biol 102: 457–468

    Article  PubMed  CAS  Google Scholar 

  7. Garcia JGN, Schaphorst KL (1995) Regulation of endothelial cell gap formation and paracellular permeability. J Invest Med 43: 117–126

    CAS  Google Scholar 

  8. Haselton FR, Heimark RL (1997) Role of cadherins 5 and 13 in the aortic endothelial barrier. J Cell Physiol 171: 243–251

    Article  PubMed  CAS  Google Scholar 

  9. Farquhar MG, Palade GE (1963) Junctional complexes in various epithelia. J Cell Biol 17: 375–412

    Article  PubMed  CAS  Google Scholar 

  10. Gumbiner BM (1996) Cell adhesion: the molecular basis of tissue architecture and morphogenesis. Cell 84: 345–357

    Article  PubMed  CAS  Google Scholar 

  11. Schneeberger EE, Lynch RD (1992) Structure, function and regulation of cellular tight junctions. Am J Physiol 262: L647–L661

    PubMed  CAS  Google Scholar 

  12. Staehelin LA (1973) Further observations on the fine structure of freeze-cleaved tight junctions. J Cell Sci 13: 763–786

    PubMed  CAS  Google Scholar 

  13. Citi S (1993) The molecular organisation of tight junctions. J Cell Biol 121: 485–489

    Article  PubMed  CAS  Google Scholar 

  14. Anderson JM, Van Itallie, CM (1995) Tight junctions and the molecular basis for regulation of paracellular permeability. Am J Physiol 269: G467–G475

    PubMed  CAS  Google Scholar 

  15. Balda MS, Matter K (1998) Tight junctions. J Cell Sci 111: 541–547

    PubMed  CAS  Google Scholar 

  16. Denker BM, Nigam SK (1998) Molecular structure and assembly of the tight junction. Am J Physiol 274: F1–F9

    PubMed  CAS  Google Scholar 

  17. Tsukita Sh, Tsukita Sa (1989) Isolation of cell-to-cell adherens junctions from rat liver. J Cell Biol 108: 31–41

    Article  PubMed  CAS  Google Scholar 

  18. Stevenson BR, Sciciliano JD, Mooseker MS, Goodenough DA (1986) Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia. J Cell Biol 103: 755–766

    Article  PubMed  CAS  Google Scholar 

  19. Rubin LL, Hall DE, Porter S, Barbu K, Cannon C, Horner HC, Janatpour M, Liaw CW, Manning K, Morales J et al (1991) A cell culture model of the blood-brain barrier. J Cell Biol 115: 1725–1735

    Article  PubMed  CAS  Google Scholar 

  20. Itoh M, Nagafuchi A, Yonemura S, Kitani-Yasuda T, Tsukita S, Tsukita S (1993) The 220-kD protein colocalizing with cadherins in non-epithelial cells is identical to ZO-1, a tight junction-associated protein in epithelial cells: cDNA cloning and immunoelectron microscopy. J Cell Biol 121: 491–502

    Article  PubMed  CAS  Google Scholar 

  21. Gumbiner BM, Lowenkopf T, Apatira D (1991) Identification of a 160 kDa polypeptide that binds to the tight junction protein ZO-1. Proc Natl Acad Sci USA 88: 3460–3464

    Article  PubMed  CAS  Google Scholar 

  22. Willott E, Balda MS, Fanning AS, Jameson B, Van Itallie C, Anderson JM (1993) The tight junction protein ZO-1 is homologous to the Drosophila discs-large tumor suppressor protein of septate junctions. Proc Natl Acad Sci USA 15: 7834–7838

    Article  Google Scholar 

  23. Jesaitis LA, Goodenough DA (1994) Molecular characterization and tissue distribution of ZO-2, a tight junction protein homologous to ZO-1 and the Drosophila discs-large tumor suppressor protein. J Cell Biol 124: 949–961

    Article  PubMed  CAS  Google Scholar 

  24. Beatch M, Jesaitis LA, Gallin WJ, Goodenough DA, Stevenson BR (1996) The tight junction protein ZO-2 contains three PDZ (PSD-95/Discs-Large/ZO-l) domains and an alternatively spliced region. J Biol Chem 271: 25723–25726

    Article  PubMed  CAS  Google Scholar 

  25. Balda MS, Anderson, JM (1993) Two classes of tight junctions are revealed by ZO-1 iso-forms. Am J Physiol 264: C918–C924

    PubMed  CAS  Google Scholar 

  26. Kurihara H, Anderson JM, Farquhar MG (1992) Diversity among tight junctions in rat kidney: glomerular slit diaphragms and endothelial junctions express only one isoform of the tight junction protein ZO-1. Proc Natl Acad Sci USA 89: 7075–7079

    Article  PubMed  CAS  Google Scholar 

  27. Hirase T, Staddon JM, Saitou M, Ando-Akatsuka Y, Itoh M, Furuse M, Fujimoto K, Tsukita Sh, Rubin LL (1997) Occludin as a possible determinant of tight junction permeability in endothelial cells. J Cell Sci 110: 1603–1613

    PubMed  CAS  Google Scholar 

  28. Sheth B, Fesenko I, Collins J, Moran B, Wild, A, Anderson JM, Fleming T (1997) Tight junction assembly during mouse blastocyst formation is regulated by late expression of ZO-1 alpha plus isoform. Development 124: 2027–2037

    PubMed  CAS  Google Scholar 

  29. Gumbiner B (1993) Breaking through the tight junction barrier. J Cell Biol 123: 1631–1633

    Article  PubMed  CAS  Google Scholar 

  30. Furuse M, Hirase T, Itoh M, Nagafuchi A, Yonemura S, Tsukita S, Tsukita S (1993) Occludin: a novel integral membrane protein localizing at tight junctions. J Cell Biol 123: 1777–1788

    Article  PubMed  CAS  Google Scholar 

  31. Ando-Akatsuka Y, Saitou M, Hirase T, Kishi M, Sakakibara A, Itoh M, Yonemura S, Furuse M, Tsukita S (1996) Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog and rat-kangaroo homologues. J Cell Biol 133: 43–47

    Article  PubMed  CAS  Google Scholar 

  32. Furuse M, Itoh M, Hirase T, Nagafuchi A, Yonemura S, Tsukita S, Tsukita S (1994) Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin at tight junctions. J Cell Biol 127: 1617–1626

    Article  PubMed  CAS  Google Scholar 

  33. Balda, MS, González-Mariscal L, Matter K, Cereijido M, Anderson JM (1993) Assembly of tight junctions: the role of diacylglycerol. J Cell Biol 123: 293–302

    Article  PubMed  CAS  Google Scholar 

  34. Itoh M, Yonemura S, Nagafuchi A, Tsukita S, Tsukita S (1991) A 220-kD undercoat-constitutive protein: its specific localization at cadherin-based cell-cell adhesion sites. J Cell Biol 115: 1449–1462

    Article  PubMed  CAS  Google Scholar 

  35. Balda MS, Whitney JA, Flores C, Gonzalez S, Cereijido M, Matter K (1996) Functional dissociation of paracellular permeability and transepithelial resistance and disruption of the apical-basolateral intramembrane diffusion barrier by expression of a mutant tight junction membrane protein. J Cell Biol 134: 1031–1049

    Article  PubMed  CAS  Google Scholar 

  36. McCarthy KM, Skare IB, Stankewich MC, Furuse M, Tsukita S, Rogers RA, Lynch RD, Schneeberger EE (1996) Occludin is a functional component of the tight junction. J Cell Sci 109: 2287–2298

    PubMed  CAS  Google Scholar 

  37. Van Itallie CM, Anderson JM (1997) Occludin confers adhesiveness when expressed in fibroblasts. J Cell Sci 110: 1113–1121

    PubMed  Google Scholar 

  38. Fanning AS, Anderson JM (1996) Protein-protein interactions: PDZ domain networks. Curr Biol 6: 1385–1388

    Article  PubMed  CAS  Google Scholar 

  39. Citi S, Sabanay H, Jakes R, Geiger B, Kendrick-Jones J (1988) Cingulin, a new peripheral component of tight junctions. Nature 333: 272–276

    Article  PubMed  CAS  Google Scholar 

  40. Zhong Y, Saitoh T, Minase T, Sawada N, Enomoto K, Mori M (1993) Monoclonal antibody 7H6 reacts with a novel tight junction associated protein distinct from ZO-1, cingulin and ZO-2. J Cell Biol 120: 477–483

    Article  PubMed  CAS  Google Scholar 

  41. Zahraoui A, Joberty G, Arpin M, Fontaine JJ, Hellio R, Tavitian A, Louvard D (1994) A small rab GTPase is distributed in cytoplasmic vesicles in non polarized cells but colo-calizes with the tight junction marker ZO-1 in polarized epithelial cells. J Cell Biol 124: 101–115

    Article  PubMed  CAS  Google Scholar 

  42. Keon BH, Schäfer. S. Kuhn C, Grund C, Franke WW (1996) Symplekin, a novel type of tight junction plaque protein. J Cell Biol 134: 1003–1018

    Article  PubMed  CAS  Google Scholar 

  43. Citi S (1992) Protein kinase inhibitors prevent junction dissociation induced by low extracellular calcium in MDCK epithelial cells. J Cell Biol 117: 169–178

    Article  PubMed  CAS  Google Scholar 

  44. Schulze C, Smales C, Rubin LL, Staddon JM (1997) Lysophosphatidic acid increases tight junction permeability in cultured brain endothelial cells. J Neuroehem 68: 991–1000

    Article  CAS  Google Scholar 

  45. Takeichi M (1995) Morphogenetic roles of classic cadherins. Curr Opin Cell Biol 7: 619–627

    Article  PubMed  CAS  Google Scholar 

  46. Overduin M, Harvey TS, Bagby S, Tong KI, Yau P, Takeichi M, Ikura M (1995) Solution structure of the epithelial cadherin domain responsible for selective cell adhesion. Science 267: 386–389

    Article  PubMed  CAS  Google Scholar 

  47. Shapiro L, Fannon AM, Kwong PD, Thompson A, Lehmann MS, Grubel G, Legrand JF, Als-Nielsen J, Colman DR, Hendrickson WA (1995) Structural basis of cell-cell adhesion by cadherins. Nature 374: 327–337

    Article  PubMed  CAS  Google Scholar 

  48. Nagar B, Overduin M, Ikura M, Rini JM (1996) Structural basis of calcium-induced E-cadherin rigidification and dimerization. Nature 380: 360–364

    Article  PubMed  CAS  Google Scholar 

  49. Heimark RL, Degner M, Schwartz SM (1990) Identification of a Ca2+-dependent cell-cell adhesion molecule in endothelial cells. J Cell Biol 110: 1745–1756

    Article  PubMed  CAS  Google Scholar 

  50. Lampugnani MG, Resnati M, Raiteri M, Pigott R, Piscane A, Houen G, Ruco LP, Dejana E (1992) A novel endothelial-specific membrane protein is a marker of cell-cell contacts J Cell Biol 118: 1511–1522

    Article  PubMed  CAS  Google Scholar 

  51. Suzuki S, Sano K, Tanihara H (1991) Diversity of the cadherin family: evidence for eight new cadherins in nervous tissue. Cell Regul 2: 261–270

    PubMed  CAS  Google Scholar 

  52. Matsuyoshi N, Toda K, Horiguchi Y, Tanaka T, Nakagawa S, Takeichi M, Imamura S (1997) In vivo evidence of the critical role of cadherin-5 in murine vascular integrity. Proc Assoc Am Physicians 109: 362–371

    PubMed  CAS  Google Scholar 

  53. Vittet D, Burchou T, Schweitzer A, Dejana E, Huber P (1997) Targeted null-mutation in the VE-cadherin gene impairs the organization of vascular-like structures in embryoid bodies. Proc Natl Acad Sci USA 94: 6273–6278

    Article  PubMed  CAS  Google Scholar 

  54. Liaw CW, Cannon C, Power MD, Kiboneka PK, Rubin LL (1990) Identification and cloning of two species of cadherins in bovine endothelial cells. EMBO J 9: 2701–2708

    PubMed  CAS  Google Scholar 

  55. Salomon D, Ayalon O, Patel-king R, Hynes RO, Geiger B (1992) Extrajunctional distribution of N-cadherin in cultured human endothelial cells. J Cell Sci 102: 7–17

    PubMed  CAS  Google Scholar 

  56. Aberle H, Schwartz H, Kemler R (1996) Cadherin-catenin complex: protein interactions and their implications for cadherin functions. J Cell Biochem 61: 514–523

    Article  PubMed  CAS  Google Scholar 

  57. Navarro P, Caveda L, Breviaro F, Mandoteanu I, Lampugnani MG, Dejana E (1995) Catenin-dependent and-independent functions of vascular endothelial cadherin. J Biol Chem 270: 30965–30972

    Article  PubMed  CAS  Google Scholar 

  58. Huber AH, Nelson WJ, Weis WI (1997) Three-dimensional structure of the armadillo repeat region of β-catenin. Cell 90: 871–882

    Article  PubMed  CAS  Google Scholar 

  59. Staddon JM, Smales C, Schulze C, Esch FS, Rubin LL (1995) pl20, a pl20-related protein (plOO) and the cadherin/catenin complex. J Cell Biol 130: 369–381

    Article  PubMed  CAS  Google Scholar 

  60. Lampugnani MG, Corada M, Cavead L, Breviario F, Ayalon O, Geiger B, Dejana E (1995) The molecular organization of endothelial cell to cell junctions: differential association of plakoglobin, β-catenin, and α-catenin with vascular endothelial cadherin (VE-cadherin). J Cell Biol 129: 203–217

    Article  PubMed  CAS  Google Scholar 

  61. Lum H, Malik AB (1994) Regulation of vascular endothelial barrier function. Am J Physiol 267: 223–241

    Google Scholar 

  62. Daniel JM, Reynolds AB (1997) Tyrosine phosphorylation and cadherin/catenin function. Bioessays 19: 883–891

    Article  PubMed  CAS  Google Scholar 

  63. Reynolds AB, Herbert L, Cleveland JL, Berg ST, Gaut JR (1992) pl20, a novel substrate of protein tyrosine kinase receptors and of p60v-src, is related to cadherin-binding factors beta-catenin, plakoglobin and armadillo. Oncogene 7: 2439–2445

    PubMed  CAS  Google Scholar 

  64. Peifer MS, Berg S, Reynolds AB (1994) A repeating amino acid motif shared by proteins with diverse cellular roles. Cell 76: 789–791

    Article  PubMed  CAS  Google Scholar 

  65. Mo YY, Reynolds AB (1996) Identification of murine pl20 isoforms and heterogeneous expression of pl20cas isoforms in human tumor cell lines. Cancer Res 56: 2633–2640

    PubMed  CAS  Google Scholar 

  66. Reynolds AB, Daniel J, McCrea P, Wheelock MJ, Wu J, Zhang Z (1994) Identification of a new catenin: the tyrosine kinase substrate pl20cas associates with E-cadherin complexes. Mol Cell Biol 14: 8333–8342

    PubMed  CAS  Google Scholar 

  67. Shibamoto S, Hayakawa M, Takeuchi K, Hori T, Miyazawa K, Kitamura N, Johnson KR, Wheelock MJ, Matusyoshi N, Takeichi M, Ito F (1995) Association of pl20, a tyrosine kinase substrate, with E-cadherin/catenin complexes. J Cell Biol 128: 949–957

    Article  PubMed  CAS  Google Scholar 

  68. Daniel JM, Reynolds AB (1995) The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or ot-catenin. Mol Cell Biol 9: 4819–4824

    Google Scholar 

  69. Lampugnani MG, Corada M, Andriopxoulou P, Esser S, Risau W, Dejana E (1997) Cell confluence regulates tyrosine phosphorylation of adherens junction components in endothelial cells. J Cell Sci 110: 2065–2077

    PubMed  CAS  Google Scholar 

  70. Huang A, Manning TM, Bandak MC, Ratau KR, Hanser KR, Silverstein SC (1993) Endothelial cell cytosolic free calcium regulates neutrophil migration across monolayers of endothelial cells. J Cell Biol 120: 1371–1380

    Article  PubMed  CAS  Google Scholar 

  71. Parkos CA (1997) Molecular events in neutrophil transepithelial migration. Bioessays 19: 865–873

    Article  PubMed  CAS  Google Scholar 

  72. Wolburg H, Neuhasu J, Kniessel U, Krauss B, Schmid E-M, Öcalan M, Farrell C, Risau W (1994) Modulation of tight junction structures in blood-brain barrier endothelial cells. J Cell Sci 107: 1347–1357

    PubMed  CAS  Google Scholar 

  73. Langeier EG, van Hinsbergh VW (1991) Norepinephrine and iloprost improve barrier function of human endothelial cell monolayers: role of cAMP. Am J Physiol 260: C1052–C1059

    Google Scholar 

  74. Goeckeler ZM, Wysolmerski RB (1995) Myosin light chain kinase-regulated endothelial contraction: the relationship between isometric tension, actin polymerization and myosin phosphorylation. J Cell Biol 130: 613–627

    Article  PubMed  CAS  Google Scholar 

  75. Warren JB, Wilson AJ, Lori RK, Coughlan ML (1993) Opposing roles of cyclic AMP in the vascular control of edema formation. FASEB J 7: 1394–1400

    PubMed  CAS  Google Scholar 

  76. Suttorp N, Ehreiser P, Hippenstiel S, Fuhrmann M, Krull M, Tenor H, Schudt C (1996) Hyperpermeability of pulmonary endothelial monolayer: protective role of phosphodi-esterase isoenzymes 3 and 4. Lung 174: 181–194

    PubMed  CAS  Google Scholar 

  77. Moolenaar WH (1995) Lysophosphatidic acid, a multifunctional phospholipid messenger. J Biol Chem 270: 12949–12952

    PubMed  CAS  Google Scholar 

  78. Kimura K, Ito M, Amano M, Chihara K, Fukata Y, Nakafuku M, Yamamori B, Feng J, Nakano T, Okawa K, Iwamatsu A, Kaibuchi K (1996) Regulation of myosin phos-phatase by Rho and Rho-associated kinase (Rho-kinase). Science 273: 245–248

    Article  PubMed  CAS  Google Scholar 

  79. Narumiya S, Ishizaki T, Wtanabe N (1997) Rho effectors and reorganization of the actin cytoskeleton. FEBS Lett 410: 68–72

    Article  PubMed  CAS  Google Scholar 

  80. Hall A (1998) Rho GTPases and the actin cytoskeleton. Science 279: 509–514

    Article  PubMed  CAS  Google Scholar 

  81. Braga VMM, Machesky L, Hall A, Hotchin NA (1997) The small GTPases Rho and Rac are required for the establishment of cadherin-dependent cell-cell contacts. J Cell Biol 137: 1421–1431

    Article  PubMed  CAS  Google Scholar 

  82. Takata K, Singer SJ (1988) Phosphotyrosine-modified proteins are concentrated at the membranes of epithelial and endothelial cells during tissue development in chick embryos. J Cell Biol 106: 1757–1764

    Article  PubMed  CAS  Google Scholar 

  83. Tsukita S, Oishi K, Akiyama T, Tamanishi Y, Yamamoto T, Tsukita S (1991) Specific proto-oncogenic tyrosine kinases of the src family are enriched in cell-to-cell adherens junction where the level of tyrosine phosphorylation is elevated. J Cell Biol 113: 867–879

    Article  PubMed  CAS  Google Scholar 

  84. Matsuyoshi N, Hamaguchi M, Taniguchi S, Nagafuchi A, Tsukita S, Takeichi M (1992) Cadherin-mediated cell-cell adhesion is perturbed by v-src tyrosine phosphorylation in metastaic fibroblasts. J Cell Biol 118: 703–714

    Article  PubMed  CAS  Google Scholar 

  85. Takeda H, Nagafuchi A, Yonemura S, Tsukita S, Behrens J, Birchmeier W, Tsukita S (1995) V-src kinase shifts the cadherin-based cell adhesion from the strong to the weak state and β-catenin is not required for the shift. J Cell Biol 131: 1839–1847

    Article  PubMed  CAS  Google Scholar 

  86. Zondag GCM, Moolenaar WH, Gebbink MFBG (1996) Lack of association between receptor protein tyrosine phosphatase RPTPji and cadherins. J Cell Biol 134: 1513–1517

    Article  PubMed  CAS  Google Scholar 

  87. Brady-Kalnay SM, Rimm DL, Tonks NK (1995) Receptor protein tyrosine phosphatase PTPμ associates with cadherins and catenins in vivo. J Cell Biol 130: 977–986

    Article  PubMed  CAS  Google Scholar 

  88. Balsamo J, Leung TC, Ernst H, Zanin MKB, Hoffman S, Lilien J (1996) Regulated binding of a PTP IB-like phosphatase to N-cadherin: control of N-cadherin-mediated adhesion by dephosphorylation of β-catenin. J Cell Biol 134: 801–813

    Article  PubMed  CAS  Google Scholar 

  89. Fuchs M, Muller T, Lerch MM, Ullrich A (1996) Association of human protein tyrosine phosphatase k with members of the Armadillo family. J Biol Chem 271: 16712–16719

    Article  PubMed  CAS  Google Scholar 

  90. Kypta RM, Su H, Reichardt LF (1996) Association between a transmembrane protein tyrosine phosphatase and the cadherin-catenin complex. J Cell Biol 134: 1519–1529

    Article  PubMed  CAS  Google Scholar 

  91. Staddon JM, Herrenknecht K, Smales C, Rubin LL (1995) Evidence that tyrosine phosphorylation may increase tight junction permeability. J Cell Sci 108: 609–619

    PubMed  CAS  Google Scholar 

  92. Ayalon O, Geiger B (1997) Cyclic changes in the organization of cell adhesions and the associated cytoskeleton, induced by stimulation of tyrosine phosphorylation in bovine aortic endothelial cells. J Cell Sci 110: 547–556

    PubMed  CAS  Google Scholar 

  93. Ratcliffe MJ, Rubin LL, Staddon JM (1997) Dephosphorylation of the cadherin-associ-ated p100/pl20 proteins in response to activation of protein kinase C in epithelial cells. J Biol Chem 272: 31894–31901

    Article  PubMed  CAS  Google Scholar 

  94. Sakakibara A, Furuse M, Ando-Akatstuka Y, Tsukita Sh (1997) Possible involvement of phosphorylation of occludin in tight junction formation. J Cell Biol 137: 1393–1401

    Article  PubMed  CAS  Google Scholar 

  95. Satoh H, Zhong Y, Isomura H, Saitoh M, Enomoto K, Sawada N, Mori M (1996) Localization of 7H6 tight junction-associated antigen along the cell border of vascular endothelial cells correlates with paracellular barrier function against ions, large molecules, and cancer cells. Exp Cell Res 222: 269–274

    Article  PubMed  CAS  Google Scholar 

  96. Albelda SM, Smith CW, Ward PA (1994) Adhesion molecules and inflammatory injury. FASEB J 8: 504–512

    PubMed  CAS  Google Scholar 

  97. Hixenbaugh EA, Goeckeler ZM, Papaiya NN, Wysolmerski RB, Silverstein SC, Huang AJ (1997) Stimulated neutrophils induce myosin light chain phosphorylation and isometric tension in endothelial cells. Am J Physiol 273: H981–H988

    PubMed  CAS  Google Scholar 

  98. Del Maschio A, Zanetti A, Corada M, Rival Y, Ruco L, Lampugnani MG, Dejana E (1996) Polymorphonuclear leukocyte adhesion triggers the disorganization of endothelial cell-to-cell adherens junctions. J Cell Biol 135: 497–510

    Article  PubMed  Google Scholar 

  99. Moll T, Dejana E, Vestweber D (1998) In vitro degradation of endothelial catenins by a neutrophil protease. J Cell Biol 140: 403–407

    Article  PubMed  CAS  Google Scholar 

  100. Lewalle JM, Bajou K, Desreux J, Mareel M, Dejana E, Noel A, Foidart JM (1997) Alteration of interendothelial adherens junctions following tumor cell-endothelial cell interaction in vitro. Exp Cell Res 237: 347–356

    Article  PubMed  CAS  Google Scholar 

  101. Sandig M, Negrou E, Rogers KA (1997) Changes in the distribution of LFA-1, catenins and F-actin during transendothelial migration of monocytes in culture. J Cell Sci 110: 2807–2818

    PubMed  CAS  Google Scholar 

  102. Gotsch, U, Borges E, Bosse R, Böggemeyer E, Simon M, Mossmann H, Vestweber D (1997) VE-cadherin antibody accelerates neutrophil recruitment in vivo. J Cell Sci 110: 583–588

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Eisai London Research Laboratories Ltd., Bernard Katz Building, University College London, Gower Street, London, WC1E 6BT, UK

    James M. Staddon

  2. 1st Department of Internal Medicine, School of Medicine, Kobe University, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe-city, 650, Japan

    Tetsuaki Hirase

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  1. James M. Staddon
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  1. Centre for Cardiovascular Biology and Medicine, School of Biomedical Sciences King’s College London, Guy’s Campus, London, SE1 9RT, UK

    Jeremy D. Pearson

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Staddon, J.M., Hirase, T. (1999). Tight junctions and adherens junctions in endothelial Cells: structure and regulation. In: Pearson, J.D. (eds) Vascular Adhesion Molecules and Inflammation. Progress in Inflammation Research. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8743-4_6

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  • DOI: https://doi.org/10.1007/978-3-0348-8743-4_6

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