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Microinjection pp 31–43Cite as

Birkhäuser

The Use of Plasmid Microinjection to Study Specific Cell Cycle Phase Transitions

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Part of the book series: Methods and Tools in Biosciences and Medicine ((MTBM))

Abstract

Progression through the eukaryotic cell cycle is a highly regulated process in which cellular proliferation is dependent on the integration of multiple signals (reviewed in, Hamel and Hanley-Hyde, 1997; Reed, 1997; Del Sal et al., 1996; Sherr, 1996; Palmero and Peters, 1996). Diverse extracellular signals such as growth factors, adhesion, and nutrient availability, as well as intracellular signals such as cell size and genomic integrity, must be monitored for proper proliferation. Mis-regulation of cellular proliferation, as occurs in cancer cells, can often be traced to mutations which influence cell cycle transitions, particularly those involved in the decision to engage in genome replication (Palmero and Peters, 1996; Hamel and Hanley-Hyde, 1997; Sherr, 1996; Hunter and Pines, 1995).

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Authors
  1. E. S. Knudsen
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Editors and Affiliations

  1. Inst. de Investigaciones Biomedicas, Calle Arturo Duperier, 4, E-28029, Madrid, Spain

    Juan Carlos Lacal  & Rosario Perona  & 

  2. University of California Cancer Center, La Jolla, CA, 92093-0636, USA

    James Feramisco

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© 1999 Springer Basel AG

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Knudsen, E.S. (1999). The Use of Plasmid Microinjection to Study Specific Cell Cycle Phase Transitions. In: Lacal, J.C., Feramisco, J., Perona, R. (eds) Microinjection. Methods and Tools in Biosciences and Medicine. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8705-2_3

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  • DOI: https://doi.org/10.1007/978-3-0348-8705-2_3

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-7643-6019-1

  • Online ISBN: 978-3-0348-8705-2

  • eBook Packages: Springer Book Archive

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