Abstract
Since the demonstration by Gurdon et al. (1971) that injection of foreign mRNA into Xenopus laevis oocytes leads to efficient and accurate translation of the encoded proteins, the oocyte system has been widely used as a tool for the study and/or cloning of receptors, ion channels and transporters. In fact, the oocyte is particularly useful as a general expression system because: i) its large size, which facilitates manipulations such as microinjection and electrode penetration, ii) the possibility to perform a variety of functional measurements using different technical approaches which include electrophysiological recordings, fluorescent dyes detection, binding of radioligands and flux measurements, and iii) the ability of the oocytes not only to express a wide variety of exogenous proteins, but also to perform postranslational processing of the newly synthesized entities (e.g. signal peptide cleavage, glycosylation, phosphorylation and subunit assembly). In this report we will focus mainly on the utility of the oocytes for expression of exogenous receptors. Some especial emphasis would be placed on studies from our laboratory centered in the expression of the thyrotropin-releasing hormone (TRH) receptor from rat adenohypophyseal cells.
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de la Peña, P., Barros, F. (1999). Functional Expression of G Protein-Coupled Receptors in Xenopus laevis Oocytes. In: Lacal, J.C., Feramisco, J., Perona, R. (eds) Microinjection. Methods and Tools in Biosciences and Medicine. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8705-2_16
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DOI: https://doi.org/10.1007/978-3-0348-8705-2_16
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