Summary
1-Aminocyclopropane- 1-carboxylate (ACC) deaminase was isolated from fungi Hansenula saturnus and Penicillium citricum, and their cDNA were analyzed Amino acid sequences of the enzymes from H. saturnus and P. citrinum showed 61% and 52% of similarity respectively to that of a bacterial enzyme. Furthermore, the alignment of these sequences indicates their similarity to the tryptophan synthase β-subunit. This became clearly evident when crystallographical analysis of ACC deaminase from H. saturnus was conducted. From the three-dimensional figure, three residues found near the PLP were chosen for preparation of the mutant enzymes.
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References
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Honma, M., Murakami, T., Ose, T., Matsui, H., Tanaka, I. (2000). Reaction and structure of 1-aminocyclopropane-1-carboxylate deaminase. In: Iriarte, A., Martinez-Carrion, M., Kagan, H.M. (eds) Biochemistry and Molecular Biology of Vitamin B6 and PQQ-dependent Proteins. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8397-9_28
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DOI: https://doi.org/10.1007/978-3-0348-8397-9_28
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