Skip to main content
SpringerLink
Log in

Mechanistic Studies Of 8-Amino-7-Oxononanoate Synthase.

  • Conference paper
Biochemistry and Molecular Biology of Vitamin B6 and PQQ-dependent Proteins

  • 259 Accesses

Summary

8-amino-7-oxononanoate synthase is a pyridoxal 5’-phosphate-dependent enzyme which catalyses the decarboxylative condensation of L-alanine with pimeloyl-CoA to form 8-amino-7-oxononanoate. Individual steps in the reaction mechanism ofEscherichia coliAONS have been probed by spectroscopy, pre-steady state kinetics and crystallography. Our results suggest that conformational transitions, induced by substrate and product binding, play an important role in catalysis.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Reference

  1. Eisenberg, M. (1987)Biosynthesis of biotin and lipoic acidVol. 1, American Society of Microbiology, Washington DC.

    Google Scholar 

  2. Alexander, F. W., Sandmeier, E., Mehta, P. K., and Christen, P. (1994)Eur. J. Biochem.219, 953–960.

    Article  PubMed  CAS  Google Scholar 

  3. Alexeev, D., Alexeeva, M., Baxter, R. L., Campopiano, D. J., Webster, S. P., and Sawyer, L. (1998)J. Mol. Biol.284, 401–419.

    Article  PubMed  CAS  Google Scholar 

  4. Toney, M. D., Hohenester, E., Cowan, S. W., and Jansonius, J. N. (1993)Science261, 756–759.

    Article  PubMed  CAS  Google Scholar 

  5. Ferreira, G. C., and Dailey, H. A. (1993)J. Biol. Chem.268, 584–590.

    PubMed  CAS  Google Scholar 

  6. Segel, I. H. (1975)Enzyme KineticsJohn Wiley & Sons, New York.

    Google Scholar 

  7. Ploux, O., and Marquet, A. (1996)Eur. J. Biochem.236, 301–308.

    Article  PubMed  CAS  Google Scholar 

  8. Dunathan, H. C. (1966)Proc. Natl. Acad. Sci. U.S.A.55, 712–716.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Edinburgh Centre for Protein Technology, Department of Chemistry, University of Edinburgh, West Mains Road, Edinburgh, EH9 3JJ, UK

    Scott P. Webster, Dominic J. Campopiano, Lisa J. Mullan & Robert L. Baxter

  2. Structural Biochemistry Group, University of Edinburgh, Swann Building, Mayfield Road, Edinburgh, EH9 3JR, UK

    Dmitriy Alexeev & Lindsay Sawyer

  3. Unit 6.03 Kelvin Campus, PanTherix Ltd, West of Scotland Science Park, Maryhill Road, Glasgow, G20 0SP, UK

    Scott P. Webster

Authors
  1. Scott P. Webster
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dmitriy Alexeev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dominic J. Campopiano
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lisa J. Mullan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Lindsay Sawyer
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Robert L. Baxter
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Division of Molecular Biology and Biochemistry School of Biological Sciences, University of Missouri, Kansas City, MO, 64110, USA

    Ana Iriarte  & Marino Martinez-Carrion  & 

  2. Department of Biochemistry, Boston University School of Medicine, 80E Concord Street, Boston, MA, 02118-2394, USA

    Herbert M. Kagan

Rights and permissions

Reprints and permissions

Copyright information

© 2000 Springer Basel AG

About this paper

Cite this paper

Webster, S.P., Alexeev, D., Campopiano, D.J., Mullan, L.J., Sawyer, L., Baxter, R.L. (2000). Mechanistic Studies Of 8-Amino-7-Oxononanoate Synthase.. In: Iriarte, A., Martinez-Carrion, M., Kagan, H.M. (eds) Biochemistry and Molecular Biology of Vitamin B6 and PQQ-dependent Proteins. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8397-9_22

Download citation

  • DOI: https://doi.org/10.1007/978-3-0348-8397-9_22

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-9549-1

  • Online ISBN: 978-3-0348-8397-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation