Summary
Lysyl oxidase is unusual amongst the copper-containing amine oxidases. It is much smaller (29kDa) and has a high cysteine content, with ten conserved cysteines. Quantitative NTSB assays show that lysyl oxidase contains five disulfide links and no free cysteines. Thus lysyl oxidase has six covalent cross-links (including one on the cofactor) that provide constraints for molecular modeling studies directed at elucidating the secondary structure of lysyl oxidase and clarifying the geometry of the active site. Computer modeling of a 34-residue, histidine-rich section of lysyl oxidase implicated as a copper-binding region indicates that this region alone does not account for the known properties of the copper-binding site, so we are now extending modeling to the 140-amino acid C-terminal region.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Kagan, H. M., Trackman, P. C. (1991) Properties and function of lysyl oxidaseAm. J. Respir. Cell Mol. Biol. 5:122–132.
Wang, S. X., Mure, M., Medzihradszky, K. F., Burlingame, A. L., Brown, D. E., Dooley, D. M., Smith, A. J., Kagan, H. M., Klinman, J. P. (1996) A cross-linked cofactor in lysyl oxidase: redox function for amino acid side chains.Science 273:1078–1084.
Gacheru, S. N., Trackman, P. C., Shah, M. A., O’Gara, C. Y., Spacciapoli, P., Greenaway, F. T., Kagan, H. M. (1990) Structural and Catalytic Properties of Copper in Lysyl Oxidase.J. Biol. Chem. 265:19022–19027.
Krebs, C. J., Krawetz, S. A. (1993) Lysyl oxidase copper-talon complex: a model.Biochim. Biophys. Acta 1202:7–12.
Riddles, P. W., Blakeley, R. L., Zerner, B. (1979) Ellman’s reagent: 5,5’-dithiobis(2nitrobenzoic acid) — a reexamination.Analytical Biochemistry 94:75–81.
Thannhauser, T. W., Konishi, Y., Scheraga, H. A. (1984) Sensitive quantitative analysis of disulfide bonds in polypeptides and proteins.Analytical Biochemistry 138:181–188.
Wiesemann, F., Tiepel, S., Krebs, B., Howeler, U. (1994) Force field calculations on the structures of transition metal complexes. 1. Application to copper(II) complexes in square planar coordination.Inorg. Chem. 33:1891–1898.
Trackman, P. C., Pratt, A. M., Wolanski, A., Tang, S.-S., Offner, G. D., Troxler, R. F., Kagan, H. M. (1990) Cloning of Rat aorta lysyl oxidase cDNA: complete codons and predicted amino acid sequence.Biochemistry 29:4863–4870.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Springer Basel AG
About this paper
Cite this paper
Greenaway, F.T., Qiu, C., Ryvkin, F. (2000). Structural Aspects of Lysyl Oxidase. In: Iriarte, A., Martinez-Carrion, M., Kagan, H.M. (eds) Biochemistry and Molecular Biology of Vitamin B6 and PQQ-dependent Proteins. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8397-9_16
Download citation
DOI: https://doi.org/10.1007/978-3-0348-8397-9_16
Publisher Name: Birkhäuser, Basel
Print ISBN: 978-3-0348-9549-1
Online ISBN: 978-3-0348-8397-9
eBook Packages: Springer Book Archive