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Gene regulation by the tetracycline-inducible Tet repressor-operator system — molecular mechanisms at atomic resolution

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Tetracyclines in Biology, Chemistry and Medicine

Abstract

At present, three-dimensional structures characterizing specific tetracycline/protein interactions are available only for the Tet repressor, TetR. This is the regulatory switch for the most common resistance mechanism against tetracyclines, Tc, in Gram-negative bacteria. Crystallographic investigations with at least 2.5 A resolution of TetR/Tc complexes [1 2] and the TetR/DNA complex [3] provide a clear view of endpoints for the functional allosteric pathway of this distinct regulatory system and reveal mechanisms that underlie TetR/Tc recognition and induced conformational changes forcing dissociation of the repressor/operator-DNA complex, TetR/tetO.

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Authors and Affiliations

  1. Institut für Chemie und Biochemie, Ernst-Moritz-Arndt Universität Greifswald, Soldmannstr. 16, Greifswald, D-17487, Germany

    Winfried Hinrichs

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  1. Winfried Hinrichs
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  2. Christiane Fenske
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Editor information

Editors and Affiliations

  1. Paratek Pharmaceuticals, 75 Kneeland St., Boston, MA, 02111, USA

    Mark Nelson

  2. Lehrstuhl für Mikrobiologie, Friedrich-Alexander Universität, Erlangen-Nürnberg Staudstr. 5, Erlangen, D-91058, Germany

    Wolfgang Hillen

  3. Division of Rheumatology, Long Island Jewish Medical Center, Suite 107 410 Lakeville Rd, New Hyde Park, NY, 11040, USA

    Robert A. Greenwald

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© 2001 Springer Basel AG

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Hinrichs, W., Fenske, C. (2001). Gene regulation by the tetracycline-inducible Tet repressor-operator system — molecular mechanisms at atomic resolution. In: Nelson, M., Hillen, W., Greenwald, R.A. (eds) Tetracyclines in Biology, Chemistry and Medicine. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8306-1_3

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  • DOI: https://doi.org/10.1007/978-3-0348-8306-1_3

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-9511-8

  • Online ISBN: 978-3-0348-8306-1

  • eBook Packages: Springer Book Archive

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