Abstract
Hyaluronan (HA) is a major component of the extracellular and pericellular matrix. It is a simple, linear glycosaminoglycan composed of thousands of disaccharide repeats of glucuronic acid and N-acetylglucosamine [1].Initially isolated from the vitreous humor, this macromolecule can be found in numerous tissues and body fluids of vertebrates, as well as in some bacteria [1].In vertebrates, HA is synthesized by any one of three hyaluronan synthases (Has) designated Has1, Has2 and Has3, respectively [2,3]. During embryogenesis, large amounts of HA are synthesized by many cell types, particularly those that are proliferating and migrating. Through specific inactivation of the mouse Has2 gene [4], we have recently shown that HA is critical for embryogenesis and is required for the maintenance and possibly the creation of extracellular matrix-defined spaces throughout the embryo, in addition to the migration of endocardial cushion cells.
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References
Laurent TC (ed) (1998) The chemistry, biology and medical applications of hyaluronan and its derivatives. Wenner-Gren International Series, Vol 72, Portland Press, London
Spicer AP, McDonald JA (1998) Characterization and molecular evolution of a vertebrate hyaluronan synthase (HAS) gene family. J Biol Chem 273: 1923–1932
Itano N, Sawai T, Yoshida M, Lenas P, Yamada Y, Imagawa M, Shinomura T, Hamaguchi M, Yoshida Y, Ohnuki Y, Miyauchi S, Spicer AP, McDonald JA, Kimata K (1999) Three isoforms of mammalian hyaluronan synthases have distinct enzymatic properties. J Biol Chem 274: 25085–25092
Camenisch TD, Spicer AP, Brehm-Gibson T, Biesterfeldt J, Augustine ML, Calabro Jr. A, Kubalak S, Klewer SE, McDonald JA (2000) Disruption of hyaluronan synthase-2 abrogates normal cardiac morphogenesis and hyaluronan-mediated transformation of epithelium to mesenchyme. J Clin Invest 106: 349–360
Gerdin B, Hallgren R (1997) Dynamic role of hyaluronan (HYA) in connective tissue activation and inflammation. J Tnt Med 242: 49–55
Knudson W, Biswas C, Li X-Q, Nemec RE, Toole BP (1989) The role and regulation of tumour-associated hyaluronan. In: D Evered, J Whelan (eds): The biology of hyaluronan. Ciba Foundation Symposium 143. Wiley, Chichester, New-York, 150–169
Delpech B, Girard N, Bertrand P, Courel M-N, Chauzy C, Delpech A (1997) Hyaluronan: fundamental principles and applications in cancer. J Int Med 242: 41–48
Fraser JRE, Laurent TC, Laurent UBG (1997) Hyaluronan: nature, distribution, functions and turnover. J Int Med 242: 27–33
Hardingham TE (1998) Cartilage: Aggrecan-link protein-hyaluronan aggregates. Glycoforum internet article http://www.glycoforum.gr.jp.
Nishida Y, Knudson CB, Eger W, Kuettner KE, Knudson W (2000) Osteogenic protein 1 stimulates cells-associated matrix assembly by normal human articular chondrocytes: up-regulation of hyaluronan synthase, CD44, and aggrecan. Arthritis Rheum 43: 206–214
Goldberg RL, Toole BP (1987) Hyaluronate inhibition of cell proliferation. Arthritis Rheum 30: 769–778
Kosaki R, Watanabe K, Yamaguchi Y (1999) Overproduction of hyaluronan by expression of the hyaluronan synthase Has2 enhances anchorage-independent growth and tumorigenicity. Cancer Res 59: 1141–1145
West DC, Hampson IN, Arnold F, Kumar S (1985) Angiogenesis induced by degradation products of hyaluronic acid. Science 228: 1324–1326
Kujawa MJ, Carrino DA, Caplan AI (1986) Substrate-bonded hyaluronic acid exhibits a size-dependent stimulation of chondrogenic differentiation of stage 24 limb mesenchymal cells in culture. Dev Biol 114: 519–528
Gilbert SF (ed) (1997) Developmental biology, 5th ed. Sinauer Associates, Inc., Sunderland, Massachusetts
Hall BK, Miyake T (2000) All for one and one for all: condensations and the initiation of skeletal development. BioEssays 22: 138–148
Knudson CB, Toole BP (1985) Changes in the pericellular matrix during differentiation of limb bud mesoderm. Dev Biol 112: 308–318
Maleski MP, Knudson CB (1996) Hyaluronan-mediated aggregation of limb bud mesenchyme and mesenchymal condensation during chondrogenesis. Exp Cell Res 225: 55–66
Watanabe H, Yamada, Y, Kimata K (1998) Roles of aggrecan, a large chondroitin sulfate proteoglycan, in cartilage structure and function. J Biochem 124: 687–693
Dowthwaite GP, Edwards JCW, Pitsillides A (1998) An essential role for the interaction between hyaluronan and hyaluronan binding proteins during joint development. J Histochem Cytochem 46: 641–651
Zou H, Wieser R, Massaguü J, Niswander L (1997) Distinct roles of type I bone morphogenetic protein receptors in the formation and differentiation of cartilage. Genes Dev 11: 2191–2203
Usui T, Amano S, Oshika T, Suzuki K, Miyata K, Araie M, Heldin P, Yamashita H (2000) Expression regulation of hyaluronan synthase in corneal endothelial cells. Invest Ophthalmol Vis Sci 41: 3261–3267
Elvin JA, Amander TC, Wang P, Wolfman NM, Matzuk MM (1999) Paracrine actions of growth differentiation factor-9 in the mammalian ovary. Molec Endocrin 13: 1035–1048
Storm EE, Kingsley DM (1999) GDF5 coordinates bone and joint formation during digit development. Developmental Biol 209: 11–27
Storm EE, Huynh TV, Copeland NG, Jenkins NA, Kingsley DM, Lee SJ (1994) Limb alterations in brachypodism mice due to mutations in a new member of the TGF-betasuperfamily. Nature 368: 639–643
Meyers EN, Lewandoski M, Martin GR (1998) An Fgf8 mutant allelic series generated by Cre-and Flp-mediated recombination. Nature Genetics 18: 136–141
Dymecki SM (1996) Flp recombinase promotes site-specific DNA recombination in embryonic stem cells and transgenic mice. Proc Natl Acad Sci USA 93: 6191–6196
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Lee, J.Y., Rountree, R.B., Kingsley, D.M., Spicer, A.P. (2002). In vivo investigation of hyaluronan and hyaluronan synthase-2 function during cartilage and joint development. In: Hascall, V.C., Kuettner, K.E. (eds) The Many Faces of Osteoarthritis. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8133-3_21
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DOI: https://doi.org/10.1007/978-3-0348-8133-3_21
Publisher Name: Birkhäuser, Basel
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