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PCR Methods and Approaches

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Book cover Techniques in Molecular Systematics and Evolution

Part of the book series: Methods and Tools in Biosciences and Medicine ((MTBM))

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Abstract

While it is possible to perform molecular systematic studies without the Poly-merase Chain reaction (PCR; [1]), there is no doubt that PCR is responsible for the tremendous explosion of the field, both in terms of the number of sequences generated and the number of taxa which have been sampled. This technique, which at its most basic can be accomplished with three water baths, a pair of hands and the necessary reagents, has made it possible to quickly and easily search entire genomes for particular sequences. Researchers using PCR have extracted genetic information from ancient samples (reviewed in [2]; see also “Enjoy 577 Ancient DNA references (or even more)” http://www.comic.sbg.ac.at/staff/jan/ancient/references.htm) and as minimal a starting material as a single cell [3]. Its effect has been felt in all fields of biology ranging from medicine and forensic science to ecology and evolutionary biology.

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Authors
  1. James Bonacum
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  2. Julian Stark
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  3. Elizabeth Bonwich
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Editor information

Editors and Affiliations

  1. Department of Entomology, American Museum of Natural History, Central Park West at 79th Street, New York, 10024-5192, NY, USA

    Rob DeSalle , Gonzalo Giribet  & Ward Wheeler ,  & 

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© 2002 Springer Basel AG

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Bonacum, J., Stark, J., Bonwich, E. (2002). PCR Methods and Approaches. In: DeSalle, R., Giribet, G., Wheeler, W. (eds) Techniques in Molecular Systematics and Evolution. Methods and Tools in Biosciences and Medicine. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8125-8_14

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  • DOI: https://doi.org/10.1007/978-3-0348-8125-8_14

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-7643-6257-7

  • Online ISBN: 978-3-0348-8125-8

  • eBook Packages: Springer Book Archive

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