Skip to main content

Abstract

Literally hundreds of protocols for DNA preparation from various sources of tissue have been published over the last few decades. To display all of these preparations would take volumes of manual space so instead we present in this chapter several of the preparations that have been used successfully in our laboratories. We also present a few “classical” procedures that are “tried and true” and nearly always work. In addition the www is an excellent source for protocols. Some forums exist for the dissemination of protocols for DNA and RNA isolation (DNA isolation protocols forums: http://www.nwfsc.noaa.gov/protocols.html, http://bric.postech.ac.kr/resources/rprotocol/; RNA isolation protocols forum: http://www.nwfsc.noaa.gov/protocols/methods/RNAMethodsMenu.html).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  2. Hillis DM, Larsen A, Davis SK, Zimmer EA (1990) Nucleic Acids III: Sequencing. In: DM Hillis and C Moritz (eds): Molecu-lar Systematics. Sinauer Associates, Inc., Sunderland, Massachusetts, 318–370

    Google Scholar 

  3. Hillis DM, Mable BK, Larson A, Davis SK et al. (1996) Nucleic Acids IV: Sequen-cing and Cloning. In: DM Hillis, C Moritz, BK Mable (eds): Molecular Systematics, second edition. Sinauer Associates, Inc., Sundland, Massachusetts, 321–381

    Google Scholar 

  4. Palumbi SR (1996) Nucleic Acids II: The Polymerase Chain Reaction. In: DM Hillis, C Moritz, BK Mable (eds): Molecular Systematics, second edition. Sinauer Associates, Inc., Sundland, Massachusetts, 205–247

    Google Scholar 

  5. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A laboratory manual, 2nd ed. 3 volumes. Cold Springs Harbor Laboratory, Cold Springs Harbor, NY

    Google Scholar 

  6. Dowling TE, Moritz C, Palmer JD (1990) Nucleic acids II: Restriction site analysis. In DM Hillis, C Moritz (eds) Molecular Systematics. Sinauer Associates, Sun¬derland, Massachusetts, 250–317

    Google Scholar 

  7. Dowling TE, Moritz C, Palmer JD, Riesberg LH (1996) Nucleic acids III: Analysis of Fragments and Restriction sites. In: DM Hillis, C Moritz, BK Mable (eds): Molecular Systematics, second edition. Sinauer Associates, Sunderland, Massa¬chusetts, 249–320

    Google Scholar 

  8. Miller SA, Dykes DD, Polesky GF (1988) A simple procedure for extracting DNA from human nucleated cells. Nuc. Acids Res. 16: 215

    Google Scholar 

  9. Coleman AW, Goff LJ (1991) DNA analysis of eukaryotic algal species. J. Phycol. 27: 463–73

    Article  Google Scholar 

  10. Jorgensen RA, Cuellar RE, Thompson WF, Kavanagh TA (1987) Structure and variation in ribosomal RNA genes of pea. Plant Mol. Biol. 8: 3–12

    CAS  Google Scholar 

  11. Palmer JD (1991) Plastid chromosomes: structure and evolution. In: L Bogorad, IK Vasil (eds): The molecular biology of plastids, Vol. 7A. Academic Press, San Diego, 5–53

    Google Scholar 

  12. Birley AJ and Croft JH (1986) Mitochondrial DNAs and phylogenetic relationships. In: SK Dutta (ed): DNA systematics, Vol. I. CRC Press, Boca Raton, Florida, 107–37

    Google Scholar 

  13. Gray MW (1989) Origin and evolution of mitochondrial DNA. Ann u. Rev. Cell Biol. 5: 25–50

    Article  CAS  Google Scholar 

  14. Ward BL, Anderson RS, Bendich M (1981) The size of the mitochondrial genome is large and variable in a family of plants (Curcurbitaceae). Cell 25: 793–803

    Article  PubMed  CAS  Google Scholar 

  15. Harris EH (1989) The Chlamydomonas Sourcebook. Academic Press, San Diego, CA., 780 pp

    Google Scholar 

  16. Nishiguchi MK, Ruby EG, McFall-Ngai MJ (1998) Competitive dominance during colonization is an indicator of coevolution in an animal-bacterial symbiosis. Appl. Environ. Microbiol. 64 (9): 3209–13

    PubMed  CAS  Google Scholar 

  17. Singer-Sam JR, Tanguay C, Riggs AD (1989) Use of Chelex to improve PCR signal from a small number of cells. Amplifications 3: 11

    Google Scholar 

  18. Walsh PS, Metzger DA, Higuchi R (1991) Chelex-100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10: 506–513

    PubMed  CAS  Google Scholar 

  19. Mundy NI, Unitt P, Woodruff DS (1997) Skin from feet of museum specimens as a non-destructive source of DNA for avian genotyping. Auk 114 (1): 126–129

    Article  Google Scholar 

  20. Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ (1979) Isolation of biolo-gically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18: 5294–5299

    Article  PubMed  CAS  Google Scholar 

  21. Phillips M, Simon C (1995) Simple, efficient, and nondestructive DNA extraction protocol for arthropods. Annl. Entomol. Soc. Amer. 88(3): 281–283

    CAS  Google Scholar 

  22. Higuchi R (1989) Simple and rapid preparation of samples for PCR. In: HA Erlich (ed): PCR technology, principles and applications for DNA amplification, Stockton Press, New York, 31–38

    Google Scholar 

  23. Giribet G, Carranza S, Baguñà J, Riutort M et al. (1996) First molecular evidence for the existence of a Tardigrada + Arthropoda clade. Mol. Biol. Evol. 13: 76–84

    Article  PubMed  CAS  Google Scholar 

  24. Struwe LM, Thiv JW, Kadereit AS-R, Pepper TJ, Motley et al. (1998) Saccifolium an endemic of Sierra de La Neblina on the Brazillian-Venezuelan frontier is related to a temperate alpine lineage of Gentianaceae. Harvard Papers in Botany 3: 199–214

    Google Scholar 

  25. Ausubel FM, Brent R, Kingston RRE, Moore D et al. (1998) Current Protocols in Molecular Biology, John Wiley and Sons, New York

    Google Scholar 

  26. Goff LJ, Moon DA (1993) PCR amplification of nuclear and plastid genes from algal herbarium specimens and algal spores. J. Phycol. 29: 381–84

    Article  CAS  Google Scholar 

  27. Chomczynski P, Sacchi N (1987) Single step method of RNA isolation by acid guanidinium iso thiocyanate-phenolchloroform extraction. Analytical Biochemistry 162, 156–159

    Article  PubMed  CAS  Google Scholar 

  28. Chomczynski P (1993) A reagent for the single-step simultaneous isolation of RNA, DNA, and proteins from cell and tissue. Biotechniques 15,532–537

    PubMed  CAS  Google Scholar 

  29. Chomczynski P (1992) Solubilization in formamide protects RNA from degradation. Nucleic Acids Res 20, 3791–2.

    Article  PubMed  CAS  Google Scholar 

  30. Sambrook J, Russell DW (2001) Molecular cloning. A laboratory manual. 3rd edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2002 Springer Basel AG

About this chapter

Cite this chapter

Nishiguchi, M.K. et al. (2002). DNA Isolation Procedures. In: DeSalle, R., Giribet, G., Wheeler, W. (eds) Techniques in Molecular Systematics and Evolution. Methods and Tools in Biosciences and Medicine. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8125-8_12

Download citation

  • DOI: https://doi.org/10.1007/978-3-0348-8125-8_12

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-7643-6257-7

  • Online ISBN: 978-3-0348-8125-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics