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Smooth Muscle Myosin: Molecule Conformation, Filament Assembly and Associated Regulatory Enzymes

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Airways Smooth Muscle: Biochemical Control of Contraction and Relaxation

Part of the book series: Respiratory Pharmacology and Pharmacotherapy ((RPP))

Abstract

Airways smooth muscle physiology and pharmacology have been extensively studied. In contrast, and probably due to the problem of obtaining enough homogenous tissue, no proper biochemical studies have been performed on even the major contractile proteins of the airways smooth muscle. For biochemical investigations avian gizzard muscle has been the most common source of smooth muscle tissue since myosin and other contractile proteins can readily be isolated and purified. Porcine stomach owing to its large muscle mass, has also been usefully employed. A higher level of endogenous, or contaminating, proteolytic activity creates problems however in obtaining some of the proteins in an undergraded state. Nevertheless, the properties of the contractile proteins in general and myosin in particular appear to be similar or identical to those of gizzard. On this basis we can assume that smooth muscle myosins share at least common major properties. In this review most of the information cited derives from studies on avian gizzard or pig stomach myosins but this should be readily applicable to myosin from airways smooth muscle.

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  1. Institute of Molecular Biology, Austrian Academy of Science, Salzburg, Austria

    Apolinary Sobieszek

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  1. Department Head Discovery Biology, Rhône-Poulenc Rorer Ltd, Dagenham Research Centre, RM10 7XS, Dagenham, Essex, England

    David Raeburn

  2. Department of Thoracic Medicine, Royal Brompton National Heart and Lung Institute, Dovehouse Street, SW3 6LY, London, England

    Mark A. Giembycz (Lecturer) (Lecturer)

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Sobieszek, A. (1994). Smooth Muscle Myosin: Molecule Conformation, Filament Assembly and Associated Regulatory Enzymes. In: Raeburn, D., Giembycz, M.A. (eds) Airways Smooth Muscle: Biochemical Control of Contraction and Relaxation. Respiratory Pharmacology and Pharmacotherapy. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-7681-0_1

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  • DOI: https://doi.org/10.1007/978-3-0348-7681-0_1

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