Abstract
In this chapter, I shall explain how to set-up a patch-clamp technique. The aim is to guide researchers in their initial steps in the field of electrophysiology, describing the instrumentation needed, as well as the technique itself. This chapter has been written assuming that the reader has a basic knowledge of electrophysiology. I pay more attention to the practical description of the instrumentation than to a theoretical analysis of the technique.
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References
E. Neher and B. Sakmann, Nature 260 (1976) 779–802.
E. Neher, B. Sakmann and J. H. Steinbach, Pflügers Arch. 375 (1978) 219–228.
P. Hamill, A. Marty, E. Neher, B. Sakmann and F. Sigworth, Pflügers Arch. 391 (1981) 85–100.
B. Sakmann and E. Neher, Single Channel Recording, Plenum Press, New York, 1995.
B. Hille, Ionic Channels of Excitable Membranes, Sinauer Associates Inc., Sunderland, 1992.
F. Sigworth in Single Channel Recording, B. Sakmann and E. Neher (eds), Plenum Press, New York, 1995, Chap. 4, pp. 95–128.
P. Horowitz and W. Hill, The Art of Electronics, Cambridge University Press, New York, 1980.
F. Conti and E. Wanke, Q. Rev. Biophys. 8 (1975) 451–506.
P. Länger, Science 178 (1972) 24–30.
C. F. Stevens, Biophys. J. 12 (1972) 1028–1047.
L. J. DeFelice, Introduction to Membrane Noise, Plenum, New York, 1981.
J. B. Johnson, Phys. Rev. 32 (1928) 97–109.
H. Nyquist, Phys. Rev. 32 (1928) 110–113.
R. D. Purves, Microelectrode Methods for Intracellular Recording and Ionophoresis, Academic Press, London, 1981.
F. Bezanilla, Biophys. J. 47 (1985) 437–441.
T. W. Stone, Microiontophoresis and Pressure Ejection, John Wiley and Sons, Chichester, 1985.
D. Colquhoun and A. G. Hawkes in Single Channel Recording, B. Sakmann and E. Neher (eds), Plenum Press, New York, 1995, Chap. 18, pp. 397–482.
D. Colquhoun and F. Sigworth in Single Channel Recording, B. Sakmann and E. Neher (eds), Plenum Press, New York, 1995, Chap. 19, pp. 483–588.
R. W. Aldrich and G. Yellen in Single Channel Recording, B. Sakmann and E. Neher (eds), Plenum Press, New York, 1983, Chap. 13, pp. 287–300.
S. H. Heinemann and F. Conti, Methods in Enzymology, 207 (1992) 131–148.
S. H. Heinemann, F. Conti and W. Stühmer, Methods in Enzymology, 207 (1992) 350–369.
A. L. Hodgkin and A. F. Huxley, J. Physiol. 117 (1952) 500–544.
O. Moran and F. Conti, Eur. Biophys. J. 18 (1990) 25–32.
B. Sakmann and E. Neher in Single Channel Recording, B. Sakmann and E. Neher (eds), Plenum Press, New York, 1995, Chap. 21, pp. 637–651.
O. Zegarra-Moran and O. Moran, Exp. Brain Res. 95 (1993) 65–68.
C. Methfessel, V. Witzermann, T. Takahashi, M. Mishina, S. Numa and B. Sakmann, Pflügers Arch. 407 (1986) 577–588.
E. Neher and R. Eckert in Calcium and Ion Channel Modulation, A. D. Grinnel, D. Armstrong and M. B. Jackson (eds), Plenum Publishing Corporation, New York, 1988, pp. 371–377.
O. B. McManus and K. L. Magleby, J. Physiol. 402 (1988) 79–120.
M. Pusch and E. Neher, Pflügers Arch. 411 (1988) 208–214.
A. L. Hodgkin, A. F. Huxley and B. Katz, J. Physiol. 116 (1952) 424–448.
F. Lin, F. Conti and O. Moran, Eur. Biophys. J. 19 (1991) 109–118.
G. Kilic’, O. Moran and E. Cherubini, Eur. J. Neurosci. 5 (1993) 65–72.
S. G. Cull-Candy, J. R. Howe and D. C. Odgen, J. Physiol. 400 (1988) 189–222.
C. Joshi and J. Fernandez, Biophys. J. 53 (1988) 885–892.
M. Lindau and E. Neher, J. Physiol. Pflügers Arch. 411 (1988) 137–146.
W. Stühmer, W. M. Roberts and W. Almers in Single Channel Recording, B. Sakmann and E. Neher (eds), Plenum Press, New York, 1983, Chap. 8, pp. 123–134.
W. Stühmer and W. Almers, Proc. Natl. Acad. Sci. U.S.A. 79 (1982) 946–950.
R. Horn and A. Marty, J. Gen Physiol. 92 (1988) 145–159.
S. J. Korn and R. Horn, J. Gen Physiol. 94 (1989) 789–812.
F. A. Edwards, A. Konnerth, B. Sakmann and T. Takahashi, Pflügers Arch. 414 (1989) 600–612.
F. J. Sigworth and E. Neher, Nature 287 (1980) 447–449.
C. M. Armstrong and F. Bezanilla, J. Gen. Physiol. 70 (1977) 567–590.
J. C. Watkins and R. H. Evans, Ann. Rev. Pharmacol. Toxicol. 21 (1981) 165–204.
M. Sciancalepore, L. Forti and O. Moran, Biochem. Biophys. Res. Comm. 165 (1989) 481–487.
M. C. Sorgato, B. U. Keller and W. Stühmer, Nature 330 (1987) 498–500.
R. Hedrich, U. I. Flügge and J. M. Fernandez, FEBS Lett. 204 (1986) 228–232.
R. Rahamimoff, S. A. DeRiemer, B. Sakmann, H. Stadler and N. Yakir, Proc. Natl. Acad. Sci. U. S. A. 85 (1988) 5310–5314.
O. Moran, G. Sandri, E. Panfili, W. Stühmer and M. C. Sorgato, J. Biol. Chem. 265 (1990) 908–913.
M. C. Sorgato and O. Moran, Crit. Rev. Biochem. Mol. Biol. 18 (1993) 127–171.
M. Criado and B. U. Keller, FEBS Lett. 244 (1987) 172–176.
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Moran, O. (1995). Patch-clamp technique. In: Brabec, V., Walz, D., Milazzo, G. (eds) Experimental Techniques in Bioelectrochemistry. Bioelectrochemistry: Principles and Practice, vol 3. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-7607-0_9
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DOI: https://doi.org/10.1007/978-3-0348-7607-0_9
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