Abstract
To many workers in the field, the classical multilocus fingerprinting approaches based on the electrophoretic separation of DNA restriction fragments followed by probing with suitable probes is now an obsolete methodology. This is because such procedures are laborious, require much technical skill, depend on high molecular weight DNA to start with and do not easily lend themselves to automation and electronic data storage. Multilocus DNA fingerprinting also requires a different statistical approach than single locus profiling (see the chapter of Krawczak, this volume). For three main reasons, however, this approach should remain in the discussion: (1) It is the method in kinship testing and trace analysis work least likely to unravel unwanted additional information on other personal genetic traits: other than with single locus techniques, the assignment of multilocus DNA fingerprint bands to alleles to loci is not obvious. (2) The shortcomings listed above are not unsurmountable in principle; technological progress may, at some future time, manage to overcome the deficits while retaining the benefits. (3) Some multilocus DNA systems work well beyond the species on which they have been developed, which is due to the fact that the target sequences of many multilocus DNA probes are both conserved and variable throughout the plant and animal kingdoms.
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Schmidtke, J. (1999). Multilocus DNA Fingerprinting. In: Epplen, J.T., Lubjuhn, T. (eds) DNA Profiling and DNA Fingerprinting. Methods and Tools in Biosciences and Medicine. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-7582-0_5
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DOI: https://doi.org/10.1007/978-3-0348-7582-0_5
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