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DNA Fingerprinting of Prokaryotic Genomes

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DNA Profiling and DNA Fingerprinting

Part of the book series: Methods and Tools in Biosciences and Medicine ((MTBM))

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Abstract

DNA fingerprinting has become such an established technology that it has featured frequently in the media and is quite well known to the general public. This is largely a result of publicity surrounding a small number of high-profile criminal or litiginous cases. What is perhaps less well known is that the technology can also be applied to the study of prokaryotic organisms, where it has many uses. These uses may be either simple or complex, and the exact technology to be used will depend largely on the question to be asked or the hypothesis to be tested. Some of the simple uses for genotyping of prokaryotes in the laboratory would be to test for contamination of cultures or alterations in strain genotype during culture or manipulation. Further uses may concern the ecology or taxonomy of prokaryotic organisms, or applications to agricultural, veterinary or medical problems. In the latter cases, it may be to determine whether a patient (animal or human) is afflicted by a mixed or single infection of any given pathogen. Many additional questions can be asked concerning the disease under investigation. Specifically, routes of transmission and index cases can be sought, outbreaks can be investigated and disease dynamics fol lowed in a broader context. These last few are examples of the new science of molecular epidemiology, a marriage between traditional epidemiology and molecular biology.

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Authors
  1. Paul D. van Helden
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Editors and Affiliations

  1. Molekulare Humangenetik, Ruhr-Universität Bochum, 44780, Bochum, Germany

    Jörg T. Epplen

  2. Institut fĂĽr Evolutionsbiologie und Ă–kologie, An der Immenburg 1, 53121, Bonn, Germany

    Thomas Lubjuhn

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© 1999 Birkhäuser Verlag

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van Helden, P.D. (1999). DNA Fingerprinting of Prokaryotic Genomes. In: Epplen, J.T., Lubjuhn, T. (eds) DNA Profiling and DNA Fingerprinting. Methods and Tools in Biosciences and Medicine. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-7582-0_1

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  • DOI: https://doi.org/10.1007/978-3-0348-7582-0_1

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-7643-6018-4

  • Online ISBN: 978-3-0348-7582-0

  • eBook Packages: Springer Book Archive

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