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Non-coding chloroplast DNA for plant molecular systematics at the infrageneric level

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Molecular Ecology and Evolution: Approaches and Applications

Part of the book series: Experientia Supplementum ((EXS,volume 69))

Summary

With primers constructed against highly conserved regions of tRNA genes (trnT UGU, trnL UAA and trnF GAA) in chloroplast DNA, we have amplified two different non-coding spacers and one intron from four species within the genus Echium L. (Boraginaceae) and from two confamilial outgroups. The trnT UGU-trnL UAA intergenic spacer contains a greater number of polymorphic sites than the trnL UAA intron or the trnL UAA-trnF GAA intergenic spacer. We analyzed a total of 11 kb of sequence data from this non-coding DNA. Total nucleotide divergence between Echium species is on the order of 1% for these regions, all of which possess infrageneric length polymorphisms. The latter two regions contain indels which occur only in the 14 Macaronesian Island endemic species of Echium studied and suggest that these may form a monophyletic group.

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References

  • Abdel-Monem, A., Watkins, N.D. and Gast, P.W. (1971a) Potassium-Argon Ages, Volcanic Stratigraphy and Geomagnetic Polarity History of the Canary Islands: Lanzarote, Fuerteventura, Gran Canada, and La Gomera. Amer. J. Sci. 271: 490–521.

    Article  CAS  Google Scholar 

  • Abdel-Monem, A., Watkins, N.D. and Gast, P.W. (1971b) Potassium-Argon Ages, Volcanic Stratigraphy and Geomagnetic Polarity History of the Canary Islands: Tenerife, La Palma, and Hierro. Amer J. Sci. 272: 805–825.

    Article  Google Scholar 

  • Aldridge, A.E. (1981) Anatomy and evolution in macaronesian Echium (Boraginaceae). Pl. Syst. Evol. 138: 9–22.

    Article  Google Scholar 

  • Bramwell, D. (1973) Studies in the Genus Echium from Macaronesia. Monogr. Biol. Canar. 4: 71–82.

    Google Scholar 

  • Bramwell, D. (1975) Some morphological aspects of the adaptive radiation of Canary Islands Echium species. Anal Inst. Bot. Cavanilles 32: 241–254.

    Google Scholar 

  • Carlquist, S. (1962) A theory of paedomorphosis in dicotyledonous woods. Phytomorphology 12: 30–45.

    Google Scholar 

  • Carlquist, S. (1970) Wood anatomy of Echium (Boraginaceae). Aliso 7: 183–199.

    Google Scholar 

  • Carlquist, S. (1974) Island Biology. Springer Verlag, New York.

    Google Scholar 

  • Chase, M.W., Soltis, D.E., Olmstead, R.G., Morgan, D., Les, D.H., Mischler, B.D., Duvall, M.R., Price, R., Hills, H.G., Qiu, Y.-L., Kron, K.A., Rettig, J.H., Conti, I., Palmer, J.D., Manhardt, J.R., Systma, K.J., Micheals, H.J., Kress, W.J., Karol, K.G., Clark, W.D., Hedrén, M., Gaut, B.S., Jansen, R.K., Kim, K.-J., Wimpee, C.F., Smith, J.F., Furnier, J.R., Strauss, S.H., Xiang, Q.-Y., Plunkett, G.M., Soltis, P.S., Swensen, S.M., Williams, S.E., Gadek, P.A., Quinn, C.J., Eguiarte, L.E., Golenberg, E., Learn, G.H., Grahm, S., Barrett, S.C.H., Dayanandan, S. and Albert, V.A. (1993) DNA sequence phylogenetics of seed plants: An analysis of nucleotide sequences from the plastid gene rbcL. Ann. Missouri Bot. Gard. 80: 528–580.

    Article  Google Scholar 

  • Clegg, M.T., Learn, G.H., and Golenberg, E.M. (1991) Molecular evolution of chloroplast DNA. In: R.K. Seiander, A.G. Clark, and T.S. Whittam (eds): Evolution at the Molecular Level. Sinauer Associates Inc., Sunderland, Massachusetts.

    Google Scholar 

  • Clegg, M. (1993) Chloroplast gene sequences and the study of plant evolution. Proc. Natl. Acad. Sci. USA 90: 363–367.

    Article  PubMed  CAS  Google Scholar 

  • Crawford, D.J., Palmer, J.D., and Kobayashi, M. (1992a) Chloroplast restriction site variation and the evolution of the annual habit in North American Coreopsis (Asteraceae). In: P.S. Soltis, D.E. Soltis and J.J. Doyle (eds): Molecular Systematics of Plants. Chapman and Hall, New York.

    Google Scholar 

  • Crawford, D.J., Stuessey, T.F., Cosner, M.B., Haines, D.W., Silva, M.O. and Baeza, M. (1992b) Evolution of the genus Dendroseris (Asteraceae: Lactuceae) on the Juan Fernandez Islands: Evidence from chloroplast and ribosomal DNA. Syst. Bot. 17: 676–682.

    Article  Google Scholar 

  • Doyle, J.J. and Doyle, J.L. (1990) Isolation of plant DNA from fresh tissue. Focus 12: 13–15.

    Google Scholar 

  • Doyle, J.J., Lavin, M. and Bruneau, A. (1992) Contributions of molecular data to papilionoid legume systematics. In: P.S. Soltis, D.E. Soltis and J.J. Doyle (eds): Molecular Systematics of Plants. Chapman and Hall, New York.

    Google Scholar 

  • Gürke, M. (1893) Borraginaceae (Asperifoliaceae). In: Engler, A. and Prantl, K. (eds): Die natürlichen Pflanzenfamilien nebst ihren Gattungen und wichtigeren Arten insbesondere der Nutzpflanzen, unter Mitwirkung zahlreicher hervorragender Fachgelehrter IV. Teil. Abt. 3a, 3b. Leipzig, Engelmann.

    Google Scholar 

  • Hiratsuka, J., Shimada, H., Whittier, R., Ishibashi, T., Sakamoto, M., Mori, M., Kondo, C., Honji, Y., Sun, C.-R., Meng, B.-Y., Li, Y.-Q., Kanno, A., Nishizawa Y., Hirai, A., Shinozaki, K. and Sugiura, M. (1989) The complete sequence of the rice (Oryza sativa) chloroplast genome: Intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals. Mol. Gen. Genet. 217: 185–194.

    Article  PubMed  CAS  Google Scholar 

  • Hohenester, A. and Weiss, W. (1993) Exkursionsflora für die Kanarischen Inseln mit Ausblick auf ganz Makaronesien. Ulmer, Stuttgart.

    Google Scholar 

  • Jansen, R.E., Michaels, H.J., Wallace, R.S., Kim, K.-J., Keeley, S.C., Watson, L.E. and Palmer, J.D. (1992) Chloroplast DNA variation in the Asteraceae: Phylogenese and evolutionary implications. In: P.S. Soltis, D.E. Soltis and J.J. Doyle (eds): Molecular Systematics of Plants. Chapman and Hall, New York.

    Google Scholar 

  • Lems, K. and Holzapfel, C.M. (1968) Evolution in the Canary Islands. I. Phylogenetic relations in the genus Echium (Boraginaceae) as shown by trichome development. Bot. Gaz. 129: 95–107.

    Article  Google Scholar 

  • Martin, W., Gierl, A. and Saedler, H. (1989) Molecular evidence for pre-Cretaceous an-giosperm origins. Nature 339: 46–48.

    Article  CAS  Google Scholar 

  • Meusel, H. (1952) Uber Wuchsformen, Verbreitung und Phylogenie einiger mediterranmitteleuropäischer Angiospermen-Gattungen. Flora 139: 333–393.

    Google Scholar 

  • Nei, M. (1987) Molecular Evolutionary Genetics. Columbia University Press, New York, pp. 254–286.

    Google Scholar 

  • Ohyama, K., Fukuzawa, H., Kohchi, T., Shirai, H., Sano, T., Sano, S., Umesono, K., Shiki, Y., Takeuchi, M., Chang, Z., Aota, S., Inokuchi, H. and H. Ozeki (1986): Chloroplast gene Organisation deduced from complete sequence of liverwort Marchantia polymorpha chloroplast DNA. Nature 32: 572–574.

    Article  Google Scholar 

  • Palmer, J.D., Jansen, R., Micheals, H.J., Chase, M.W. and Manhardt, J.R. (1988) Chloroplast DNA variation and plant phylogeny. Ann. Missouri Bot. Gard. 75: 1180–1206.

    Article  Google Scholar 

  • Palmer, J.D. (1991) Plastid chromosomes: Structure and evolution. In: L. Bogorad and I.K. Vasil (eds): Cell Culture and Somatic Cell Genetics of Plants. Vol. 7A. Academic Press, San Diego.

    Google Scholar 

  • Palmer, J.D. (1992) Mitochondrial DNA in plant systematics: Applications and limitations. In: P.S. Soltis, D.E. Soltis and J.J. Doyle (eds): Molecular Systematics of Plants. Chapman and Hall, New York.

    Google Scholar 

  • Seibert, J. (1978) Fruchtanatomische Untersuchungen an Lithospermeae (Boraginaceae). Diss. Bot. 44.

    Google Scholar 

  • Shinozaki, K., Ohme, M., Tanaka, M., Wakasugi, T., Hayashida, N., Matsubayashi, T., Zaita, N., Chunwongse, J., Obokata, J., Yamaguchi-Shinozaki, K., Ohto, C., Torazawa, K., Meng, B.-Y., Sugita, M., Deno, H., Kamogashira, T., Yamada, K., Kusuda, J., Takaiwa, F., Kato, A., Tohdoh, N., Shimada, H. and Sugiura, M. (1986) The complete nucleotide sequence of the tobacco chloroplast genome: Its gene organisation and expression. EMBO J. 5: 2043–2049.

    PubMed  CAS  Google Scholar 

  • Soltis, D.E., Soltis, P.S. and Milligan, B.G. (1992) Intraspecific chloroplast DNA variation: Systematic and phylogenetic implications. In: P.S. Soltis, D.E. Soltis and J J. Doyle (eds): Molecular Systematics of Plants. Chapman and Hall, New York.

    Chapter  Google Scholar 

  • Taberlet, P., Gielly, L., Pautou, G. and Bouvet, J. (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol. Biol 17: 1105–1109.

    Article  PubMed  CAS  Google Scholar 

  • Valdebenito, H., Stuessey, T.F. and Crawford, C.M. (1992) Evolution of Erigeron (Compositae) in the Juan Fernandez Islands, Chile. Syst. Bot. 17: 470–480.

    Article  Google Scholar 

  • Wolfe, K.H., Li, W.-H. and Sharp, P. (1987) Rates of nucleotide substitution vary greatly among plant mitochondrial, chloroplast, and nuclear DNAs. Proc. Natl. Acad. Sci. USA 84: 9054–9058.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institut für Genetik, Technische Universität Braunschweig, Spielmannstrasse 7, Postfach 3329, D-38023, Braunschweig, Germany

    U.-R. Böhle, R. Cerff & W. F. Martin

  2. Institut für Systematische Botanik und Pflanzengeographie, Freien Universität Berlin, Fachbereich 23 Biologie, Altensteinstr. 6, D-14195, Berlin-Dahlem, Germany

    H. Hilger

Authors
  1. U.-R. Böhle
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  2. H. Hilger
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  3. R. Cerff
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  4. W. F. Martin
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Editor information

Editors and Affiliations

  1. Zoologisches Institut, Universität Frankfurt, Siesmayerstr. 70, D-60054, Frankfurt, Germany

    B. Schierwater  & B. Streit  & 

  2. Department of Biology, Yale University, 165 Prospect St., New Haven, CT, 06511, USA

    G. P. Wagner

  3. Department of Entomology, American Museum of Natural History, 79th Street at Central Park West, New York, NY, 10024, USA

    R. DeSalle

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© 1994 Springer Basel AG

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Böhle, UR., Hilger, H., Cerff, R., Martin, W.F. (1994). Non-coding chloroplast DNA for plant molecular systematics at the infrageneric level. In: Schierwater, B., Streit, B., Wagner, G.P., DeSalle, R. (eds) Molecular Ecology and Evolution: Approaches and Applications. Experientia Supplementum, vol 69. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-7527-1_23

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  • DOI: https://doi.org/10.1007/978-3-0348-7527-1_23

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-7529-5

  • Online ISBN: 978-3-0348-7527-1

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