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Purification and characterization of scatter factor

  • Ermanno Gherardi
  • Arnold Coffer
Chapter
Part of the Experientia Supplementum book series (EXS, volume 59)

Summary

Scatter factor is a fibroblast-derived protein which disrupts and scatters epithelial colonies and enhances the local movement of individual epithehal and endothehal cells. The factor purified from mouse fibroblasts by cation-exchange and reverse phase chromatography is a dimer of 57 kD and 30 kD protein subunits (A and B subunits), is active at picomolar concentrations and requires intact intra- and/or inter-chain disulphide bonds for activity. In serum-free conditioned medium the factor is highly aggregated but in the presence of high-salt buffers or protein denaturants elutes from gel filtration columns with an apparent Mr of ~ 50 kD. From a combination of molecular sieving and ultracentrifugation studies, a calculated Mr of 61.4 kD is obtained for native mouse scatter factor, a value which agrees well with the Mr estimates obtained by SDS-PAGE (62-67 kD). Mouse fibroblast scatter factor is a heparin-binding, basic protein (pI 8.5-9.5) which contains N-linked carbohydrates which are not, however, essential for activity. The factor has no metallo- or serine protease activity and there is no evidence so far that its junctional-breaking activity involves proteolytic cleavage of surface molecules on target cells. Scatter factor is either identical or closely related to hepatocyte growth factor/hepatopoietin A (a potent mitogen for rat hepatocytes recently purified from human and rabbit serum and rat platelets). The factor is thus an effector of mesenchymal-epithelial interactions which affects the movement or the growth of different epithelia.

Keywords

Hepatocyte Growth Factor MDCK Cell Scatter Factor Potent Mitogen Serine Protease Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag Basel/Switzerland 1991

Authors and Affiliations

  • Ermanno Gherardi
    • 1
    • 2
  • Arnold Coffer
    • 1
    • 3
  1. 1.Imperial Cancer Research Fund LaboratoriesUK
  2. 2.Department of Medicine, Addenbrooke’s HospitalCambridge UniversityCambridgeUK
  3. 3.Protein Isolation and Cloning LaboratoryLincoln’s Inn FieldLondonEngland

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