Skip to main content
SpringerLink
Log in
Book cover

Cell Motility Factors pp 1–16Cite as

Molecular analysis of amoeboid chemotaxis: Parallel observations in amoeboid phagocytes and metastatic tumor cells

  • Chapter

Part of the book series: Experientia Supplementum ((EXS,volume 59))

Summary

Metastasis is a multistep process in which amoeboid Chemotaxis plays a key role in the movement of tumor cells into and out of vessels. On a molecular level, much of what is known about amoeboid Chemotaxis has been learned through work with Dictyostelium discoideum, a lower eukaryotic amoeboid phagocyte. One of the first and most crucial events to occur in the actin cytoskeleton following chemotactic stimulation is activation of actin nucleation. This is followed by incorporation of specific actin cross-linking proteins into the cytoskeleton, proteins which are implicated in the extension of pseudopods and filopods. Together, these events have been termed the Cortical Expansion Model for amoeboid Chemotaxis. Detailed biochemical analysis has implicated a new actin-capping protein and has shown that one of the cross-linking proteins is Elongation Factor la, suggesting a link between Chemotaxis and growth control. Preliminary data from parallel studies on neoplastic cells are presented.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Auerbach, R., Lu, W. C., Pardon, E., Gumkowski, F., Kaminska, G., and Kaminski, M. (1987) Specificity of adhesion between murine tumor cells and capillary endothehum: an in vitro correlate of preferential metastasis in vivo. Cancer Res. 47: 1492–1496.

    Google Scholar 

  • Aznavoorian, S., Stracke, M. L., Krutzsch, H., Schiffman, E., and Liotta, L. A. (1990) Signal transduction for Chemotaxis and haptotaxis by matrix molecules in tumor cells. J. Cell Biol. 110: 1427–1438.

    Article  Google Scholar 

  • Blood, C. H., Sasse, J., Brodt, P., and Zetter, B. R. (1988) Identification of a tumor cell receptor for VGVAPG, an elastin derived chemotactic peptide. J. Cell Biol. 107: 1987–1993.

    Article  Google Scholar 

  • Blood, C. H., and Zetter, B. R. (1989) Membrane-bound protein kinase C modulates receptor affinity and chemotactic responsiveness of Lewis lung carcinoma sublines to an elastin-derived peptide. J. Biol. Chem. 264: 10614–10620.

    Google Scholar 

  • Bonner, J. T., Barkley, D. S., Hall, E. M., Konign, T. M., Mason, J. W., O’Keefe, G. 3rd, and Wolfe, P. B. (1969) Acrasin, acrasinase, and the sensitivity to acrasin in Dictyostelium discoideum. Dev. Biol. 20: 72–87.

    Article  Google Scholar 

  • Bresnick, A., Warren, V., and Condeehs, J. (1989) Identification of a short sequence essential for actin binding by Dictyostehum ABP-120. J. Biol. Chem. 265: 9236–9240.

    Google Scholar 

  • Brodt, P. (1986) Characterization of two highly metastatic variants of Lewis lung carcinoma with different organ specificities. Cancer Res. 46: 2442–2448.

    Google Scholar 

  • Chelberg, M. K., McCarthy J. B., Skubitz, A. P. M., Furcht, L. T., and Tsilibary E. C. (1990) Characterization of a synthetic peptide from type IV collagen that promotes melanoma cell adhesion, spreading, and motility. J. Cell Biol. 111: 262–270.

    Article  Google Scholar 

  • Condeelis, J., and Taylor, D. (1977) Control of gelation, solation and contraction in extracts from Dictyostelium discoideum. J. Cell Biol. 74: 901–927.

    Article  Google Scholar 

  • Condeelis, J., Vahey, M., Carboni, J., Demey, J., and Ogihara, S. (1984) Properties of the 120,000 and 95,000 dalton actin binding proteins from Dictyostelium discoideum and their possible functions in assembling the cytoplasmic matrix. J. Cell Biol. 99: 119s-126s.

    Article  Google Scholar 

  • Condeelis, J., Hall, A., Bresnick, A., Warren, V., Hock, R., Bennett, H., and Ogihara, S. (1988) Actin polymerization and pseudopod extension during amoeboid Chemotaxis. Cell Mot. Cytoskel. 10: 77–90.

    Article  Google Scholar 

  • Condeelis, J., and Hall, A. (1990) Measurement of actin polymerization and cross-linking in agonist stimulated cells. Meth. Enzymol. 196: 486–496.

    Article  Google Scholar 

  • Dano, K., Andreasen, P. A., Grondahl-Hansen, J., Kristensen, P., Nielsen, L. S., and Skriver, L. (1985). Plasminogen activators, tissue degradation, and cancer. Adv. Cancer. Res. 44: 139–266.

    Article  Google Scholar 

  • Demma, M., Warren, V., Hock, R., Dharmawardhane, S., and Condeelis, J. (1990) Isolation of an abundant 50,000 dalton actin filament bundling protein from Dictyostelium amoebae. J. Biol. Chem. 265: 2286–2291.

    Google Scholar 

  • Detmers, P., Goodenough, U., and Condeelis, J. (1983) Elongation of the fertilization tubules in Chlamydomonas: New observations on the core microfilaments and the effect of transient intracellular signals on their structural integrity. J. Cell. Biol. 97: 522–532.

    Article  Google Scholar 

  • Devreotes P. N., and Zigmond, S. H. (1988) Chemotaxis in eukaryotic cells: A focus on leukocytes and Dictyostelium. Ann. Rev. Cell Biol. 4: 649–686.

    Article  Google Scholar 

  • Dharmawardhane, S., Warren, V., Hall, A., and Condeelis, J. (1989a) Changes in the association of actin binding proteins with the actin cytoskeleton during stimulation of Dictyostelium discoideum. Cell Motil. Cytoskel. 13: 57–63.

    Article  Google Scholar 

  • Dharmwardhane, S., Demma, M., Warren, V., and Condeelis, J. (1989b) Regulation of ABP-50, an actin-binding protein from Dictyostelium discoideum. J. Cell Biol. 109: 273a.

    Google Scholar 

  • Eckert, B., Warren, V., and Rubin, R. (1977) Structural and biochemical aspects of cell motility in amebas of Dictyostelium discoideum. J. Cell Biol. 72: 339–350.

    Article  Google Scholar 

  • Hall, A., Schlein, A., and Condeelis, J. (1988) Relationship of pseudopod extension to chemotactic hormone-induced actin polymerization in amoeboid cells. J. Cell Biochem. 37: 285–299.

    Article  Google Scholar 

  • Hall, A. L., Warren, V., Dharmawardhane S., and Condeelis, J. (1989a) Identification of actin nucleation activity and polymerization inhibitor in amoeboid cells: Their regulation by chemotactic stimulation. J. Cell Biol. 109: 2207–2213.

    Article  Google Scholar 

  • Hall, A. L., Warren, V., and Condeelis J. (1989b). Transduction of the chemotactic signal to the actin cytoskeleton of Dictyostelium discoideum. Dev. Biol. 136: 517–525.

    Article  Google Scholar 

  • Inoue, S., and Tilney, L. (1982) Acrosomal reaction of thyone sperm. I. Changes in the sperm head visualized by high resolution video microscopy. J. Cell. Biol. 93: 812–819.

    Article  Google Scholar 

  • Juliano, R. L. (1987) Membrane receptors for extracellular matrix macromolecules: relationship to cell adhesion and tumor metastasis. Biochim. Biophys. Acta 907: 261–278.

    Google Scholar 

  • Kao R. T., and Stern, R. (1986) Elastases in human breast carcinoma cell lines. Cancer Res. 46: 1355–1358.

    Google Scholar 

  • Knecht, D., Kern, H., Wessels, D., Soil, D., Cox, D., and Condeelis, J. (1990) Targeted disruption of the ABP-120 gene leads to cells with altered motility. J. Cell Biol. 111: 7a.

    Google Scholar 

  • Kumagai, A., Pupillo, M., Gundersen, R., Miake-Lye, R., Devreotes, P., and Firtel, R. (1989) Regulation and function of G-alpha protein subunits in Dictyostelium. Cell 57: 265–275.

    Article  Google Scholar 

  • Liotta, L. A., Kleinerman, J., and Saidel, G. M. (1974) Quantitative relationships of intravascular tumor cells, tumor vessels, and pulmonary metastases following tumor implantation. Cancer Res. 34: 997–1004.

    Google Scholar 

  • Liotta, L. A., Tryggvason, K., Garbisa, S., Hart, I., Foltz, C. M., and Shafie, S. (1980) Metastatic potential correlates with enzymatic degradation of basement membrane collagen. Nature 284: 67–68.

    Article  Google Scholar 

  • Liotta, L. A., Mandler, R., Murano, G., Katz, D. A., Gordon, R. K., Chiang, P. K., and Schiffmann, E. (1986) Tumor autocrine motility factor. Proc. Nat. Acad. Sci. USA 83: 3302–3306.

    Article  Google Scholar 

  • Liotta, L. A., and Schiffman, E. (1988) Tumour motility factors. Cancer Surv. 7: 632–652.

    Google Scholar 

  • Liotta, L. A., and Stetler-Stevenson, W. (1989) Metalloproteinases and mahgnant conversion: does correlation imply causahty? J. Natl. Cancer Inst. 81: 556–557.

    Article  Google Scholar 

  • Liu, G., and Newell, P. (1988) Evidence that cGMP regulates myosin interaction with the cytoskeleton during Chemotaxis of Dictyostelium. J. Cell Sci. 90: 123–129.

    Google Scholar 

  • Magro, C., Orr, F. W., Manishen, W. J., Sivamanthan, K., and Mokashi, S. S. (1985) Adhesion, Chemotaxis, and aggregation of Walker carcinosarcoma cells in response to products of resorbing bone. J. Natl. Cancer Inst. 74: 829–838.

    Google Scholar 

  • McCarthy, J. B., Basara, M. L., Palm, S. L., Sas, D. F., and Furcht, L. T. (1985) The role of cell adhesion proteins - laminin and fibronectin - in the movement of malignant and metastatic cells. Cancer Metastasis Rev. 4: 125–152.

    Article  Google Scholar 

  • McCarthy J. B., Hagen, S. T., and Furcht, L. T. (1986) Human fibronectin contains distinct adhesion — and motility-promoting domains for metastatic melanoma cells. J. Cell. Biol. 102: 179–188.

    Article  Google Scholar 

  • Mensing H., Albini, A., Krieg, T., Pontz, B. F., and Muller, P. K. (1984) Enhanced Chemotaxis of tumor-derived and virus-transformed cells to fibronectin and fibroblast-conditioned medium. Int. J. Cancer 33: 43–48.

    Article  Google Scholar 

  • Moldave, K. (1985) Eukaryotic protein synthesis. Ann. Rev. Biochem. 54: 1109–1149.

    Article  Google Scholar 

  • Mundy, G. R., De Martino, S., and Rowe, D. W. (1981) Collagen and collagen-derived fragments are chemotactic for tumor cells. J. Clin. Invest. 68: 1102–1105.

    Article  Google Scholar 

  • Nakajima, M., Irimura, T., Di Ferrante, D., Di Ferrante, N., and Nicolson, G. L. (1983) Heparan sulfate degradation: relation to tumor invasive and metastatic properties of mouse B16 melanoma sublines. Science 220: 611–613.

    Article  Google Scholar 

  • Noegel, A., Rapp, S., Lottspeich, F., Schleicher, M., and Stewart, M. (1989) The Dictyostelium gelation factor shares a putative actin binding site with A-actinins and dystrophin and also has a rod domain containing six 100 residue motifs that appear to have a cross-beta conformation. J. Cell Biol. 109: 607–618.

    Article  Google Scholar 

  • Ogihara, S., Carboni, J., and Condeelis, J. (1988) Electron miroscopic localization of Myosin II and ABP-120 in the cortical actin matrix of Dictyostelium amoebae using IgG-gold conjugates. Dev. Gen. 9: 505–520.

    Article  Google Scholar 

  • Oster, G. (1984) On the crawling of cells. J. Embryol. Exp. Morph. 83 Supplement: 329–364.

    Google Scholar 

  • Oster, G. (1988) Biophysics of the leading lamella. Cell Mot. Cytoskel. 10: 164–171.

    Article  Google Scholar 

  • Sauterer, R. A., Eddy, R. J., Hall, A. L., and Condeehs, J. S. (1990) Purification and characterization of aginactin, a newly identified agonist-regulated capping protein from Dictyostelium amoebae. J. Cell Biol. 111: 162a.

    Google Scholar 

  • Segall, J. (1988) Quantification of motility and area changes of Dictyostelium discoideum amoebae in response to chemoattractants. J. Muse. Res. Cell. Mot. 9: 481–490.

    Article  Google Scholar 

  • Segall, J. (1990a) Mutational studies of amoeboid Chemotaxis using Dictyostelium discoideum. In: J. Armitage and J. Lackie (eds), Biology of the Chemotactic Responses. Cambridge University Press, Cambridge.

    Google Scholar 

  • Segall, J. (1990b) The role of GMP in chemotactic responses of Dictyosteium amoebae. In prep.

    Google Scholar 

  • Senior, R. M., Griffin, G. L., Mecham, R. P., Wrenn, D. S., Prasdad, K. U., and Urry, D. W. (1984) Val-Gly-Val-Ala-Pro-Gly, a repeating peptide in elastin, is chemotactic for fibroblasts and monocytes. J. Cell Biol. 99: 870–874.

    Article  Google Scholar 

  • Sloane, B. F., Honn, K. V., Sadler, J. G., Turner, W. A., Kimpson, J. J., and Taylor, J. D. (1982) Cathepsin B activity in B16 melanoma cells: a possible marker for metastatic potential. Cancer Res. 42: 980–986.

    Google Scholar 

  • Stracke, M. L. Engel, J. D., Wilson, L. W., Rechler, M. M., Liotta, L. A., and Schiffman, E. (1989) The type I IGF receptor is a motility receptor in human melanoma cells. J. Biol. Chem. 264: 21544–21549.

    Google Scholar 

  • Tilney, L., and Inoue, S. (1982) Acrosomal reaction of thyone sperm II. The kinetics and mechanisms of acrosomal process elongation. J. Cell Biol. 93: 820–827.

    Article  Google Scholar 

  • Trinkus, J. (1984) Cells into Organs: The Forces that Shape the Embryo, “2nd ed. Englewood Cliffs, NJ: Prentice Hall.

    Google Scholar 

  • Vlodavsky, I., Fuks, Z., Bar-Ner, M., Ariav, Y., and Schirrmacher, V. (1983) Lymphoma cell-mediated degradation of sulfated proteoglycans in the subendothelial extracellular matrix: relationship to tumor cell metastasis. Cancer Res. 43: 2704–2711.

    Google Scholar 

  • Weiss, L., Orr, F. W., and Honn, K. V. (1989) Interactions between cancer cells and the micro vasculature: a rate-regulator for metastasis. Clin. Exp. Metastasis 7: 127–67.

    Article  Google Scholar 

  • Wessels, D. (1990) The role of actin and myosin in intracellular movement, cell motihty and cell shape in Dictyostelium Chemotaxis. Ph.D. thesis. University of Iowa.

    Google Scholar 

  • Wessels, D., Schroeder, N., Voss, E., Hall, A., Condeehs, J., and Soil, D. (1989) cAMP-mediated inhibition of intracellular particle movement and actin reorganization in Dictyostelium. J. Cell. Biol. 109: 2841–2851.

    Article  Google Scholar 

  • Wolosewick, J., and Condeelis, J. (1986) Fine structure of gels prepared from an actin binding protein and actin. J. Cell. Biochem. 30: 227–243.

    Article  Google Scholar 

  • Yang, F., Demma, M., Warren, V., Dharwardhane, S., and Condeelis, J. (1990) Identification of an actin binding protein from Dictyostelium as elongation factor la. Nature: 347: 494–496.

    Article  Google Scholar 

  • Yusa, T., Blood, C. H., and Zetter, B. R. (1989) Tumor cell interactions with elastin: implications for pulmonary metastases. Am. Rev. Respir. Dis. 140: 1458–1462.

    Article  Google Scholar 

  • Zeydel, M., Nakagawa, S., Biempica, L., and Takahashi, S. (1986) Collagenase and elastase production by mouse mammary adenocarcioma primary cultures and cloned cells. Cancer Res. 46: 6438–6445.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, Albert Einstein College of Medicine, 10461, Bronx, NY, USA

    Joan G. Jones

  2. Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 10461, Bronx, NY, USA

    Jeffrey Segall & John Condeelis

Authors
  1. Joan G. Jones
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jeffrey Segall
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. John Condeelis
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Radiation Oncology, Long Island Jewish Medical Center, 270-05 76th Avenue, 11042, New Hyde Park, NY, USA

    I. D. Goldberg M.D.

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Birkhäuser Verlag Basel/Switzerland

About this chapter

Cite this chapter

Jones, J.G., Segall, J., Condeelis, J. (1991). Molecular analysis of amoeboid chemotaxis: Parallel observations in amoeboid phagocytes and metastatic tumor cells. In: Goldberg, I.D. (eds) Cell Motility Factors. Experientia Supplementum, vol 59. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7494-6_1

Download citation

  • DOI: https://doi.org/10.1007/978-3-0348-7494-6_1

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-0348-7496-0

  • Online ISBN: 978-3-0348-7494-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation