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Determination of Rate Constants for Antioxidant Activity and use of the Crocin Assay

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Lipid-Soluble Antioxidants: Biochemistry and Clinical Applications

Part of the book series: Molecular and Cell Biology Updates ((MCBU))

Summary

Antioxidative activity of a given substance can be inferred from the protective effect under in vivo oxidative stress conditions. To quantitate such effects, however, the exact scavenging rate constants for the various oxygen radicals have to be determined.

In the following, two methods to obtain such data will be presented in detail: (1) pulse radiolysis in combination with kinetic spectroscopy to directly monitor build-up and decay of antioxidant radicals and (2) competition kinetics for indirect evaluation of scavenging rates. The most versatile substance for such competition studies, especially for alkoxyl and peroxyl radicals, has turned out to be the water-soluble carotenoid crocin.

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Author information

Authors and Affiliations

  1. Inst. Strahlenbiologie, GSF Research Center, 8042, Neuherberg, Germany

    Wolf Bors, Christa Michel & Manfred Saran

Authors
  1. Wolf Bors
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  2. Christa Michel
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  3. Manfred Saran
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Editor information

Editors and Affiliations

  1. Malaysian Palm Oil Promotion Council, Bangunan Getah Asli 148 Jalan Ampang, 50450, Kuala Lumpur, Malaysia

    A. S. H. Ong

  2. Dept. of Molecular and Cell Biology 251 Life Science Addition, Membrane Bioenergetics Group, Berkeley, California, 94720, USA

    L. Packer

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© 1992 Birkhäuser Verlag, Basel/Switzerland

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Bors, W., Michel, C., Saran, M. (1992). Determination of Rate Constants for Antioxidant Activity and use of the Crocin Assay. In: Ong, A.S.H., Packer, L. (eds) Lipid-Soluble Antioxidants: Biochemistry and Clinical Applications. Molecular and Cell Biology Updates. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7432-8_5

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  • DOI: https://doi.org/10.1007/978-3-0348-7432-8_5

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-0348-7434-2

  • Online ISBN: 978-3-0348-7432-8

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