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Lipid-Soluble Antioxidants: Biochemistry and Clinical Applications pp 515–534Cite as

Dihydrolipoic Acid Is Protective Against Reperfusion Injury

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Part of the book series: Molecular and Cell Biology Updates ((MCBU))

Summary

Hypoxia/ischemia and reoxygenation/reperfusion were studied in rat hearts and rat hind limbs. Free radicals are known to be generated through these events and to cause complications. In order to reduce hypoxic/ischemic and especially reoxygenation/reperfusion injury we ameliorated (re)perfusion conditions including the treatment with dihydrolipoic acid (DHL).

  1. 1.

    In isolated working rat hearts DHL, if added into the perfusion buffer at 0.3μM concentration, keeps the pH higher (7.15) during hypoxia as compared to controls (6.98). The compound accelerates the recovery of the aortic flow and stabilizes it during reoxygenation. With DHL, ATPase activity is reduced, ATP synthesis is increased and phosphocreatine contents are higher than in controls. Creatine kinase activity is maintained during reoxygenation in the DHL treated series.

  2. 2.

    Isolated rat hind limbs were stored for 4h in a moist chamber at 18°C. Controls were perfumed for 30 min. with a modified Krebs-Henseleit buffer at 60 mm Hg followed by 30 min. Krebs-Henseleit perfusion at 100 mm Hg. The DHL group contained 8 μM DHL in the modified reperfusate. With DHL, recovery of the contractile function was 49 % (va. 34 % in controls) and muscle flexibility was maintained whereas it decreased by 15 % in the controls. Release of creatine kinase was significantly lower with DHL treatment.

DHL is efficient in reducing reoxygenation injury in isolated working rat hearts and reperfusion damage in isolated ischemic rat hind limbs.

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References

  • Alger, J.R. and Shulman, R.G. (1984) Quart. Rev. Bioshys. 17, 83–124

    Article  Google Scholar 

  • Assadnazari, B., Zimmer, G. Freisleben, H.-J., Werk, W. and Leibfiritz, D. Cardioprotective efficiency of dihydrolipoic acid in working rat hearts during hypoxia and reoxygenation: 31P NMR investigations (submitted for publication)

    Google Scholar 

  • Boyersdorf, F., Fuchs, J., Eberhardt, B., Stander, M. and Zimmer, G. (1989a) Arzneim. Forsch./Drug Res. 39, 46–49

    Google Scholar 

  • Beyersdorf, F., Matheis, G., Krüqer, S., Hanselmann, A., Freisleben, H.-J., Zimmer, G. and Satter, P. (1989b) J. Vasc. Surg. 9, 757–766

    Google Scholar 

  • Bittl, J.A. and Ingwall, J.S. (1985) J. Biol. Chem. 260, 3512–3517.

    Google Scholar 

  • Brindle, X.M. (1988) Progress in NMR spectroscopy 20, 257–293.

    Article  Google Scholar 

  • Bunik, V.I., Buneeva, O.A. and Gomazkova, V.S. (1990) FEBS Lett. 269, 252–254

    Article  Google Scholar 

  • Ellman, G.L. (1959) Arch. Biochem. Biophys. 82, 70–77

    Article  Google Scholar 

  • Freisleben, B.-J., Fuchs, J., Mainka, L. and Zimmer, G. (1988) Arch. Biochems. Biophys. 266, 89–97

    Article  Google Scholar 

  • Freisleben, B.-J., Kagan, V.E. and Packer, L. (1991) 1st ISP Congress, Moscow, Book of Abstracts, pp. 228–229

    Google Scholar 

  • Freisleben, B.-J., Kriege, B., Clarke, C., Beyersdorf, F. and Zimmer, G. (1991) Arzneim. Forsch./Drug Res. 41, 81–88.

    Google Scholar 

  • Freisleben, B.-J. and Zimmer, G. (1986) Eur. J. Biochem. 160, 155–161

    Article  Google Scholar 

  • Freisleben, H.-J. and Zimmer, G. (1989) In: Advances in Myochemistry, vol.2 (G. Benzi, Ed) J. Libbey Ltd., London/Paris, pp. 51–52

    Google Scholar 

  • Fuchs, J., Mainka, L. and Zimmer, G. (1985a) Arzneim. Forsch./Drug Res. 35, 1394–1402

    Google Scholar 

  • Fuchs, J., Veit, P. and Zimmer, G. (1985b) Basic Res. Cardiol. 80, 231–240

    Article  Google Scholar 

  • Gascoigne, I.M. and Radda, G.K. (1967) Biochim. Biophys. Acta 131, 498–507

    Article  Google Scholar 

  • Hanselmann, A., Beyersdorf, F., Hatheis, G., Wildhirt, A., Gauss, A., Oremek, G., Krüger, S., Zinner, G., Freisleben, H.-J. and Satter, P. (1990) Z. Herz-, Thorax-, Gefäßhir. 4, 913–920

    Google Scholar 

  • Hyslop, P.A., Hinshaw, D.R., Halsey, W.A., Jr., Schraufstätter, LU., Sauerheber, R.D., Spragg, R.G., Jackson, J.H. and Cochrane, C.G. (1988) J. Biol. Chem. 263, 1665–1675

    Google Scholar 

  • Jocelyn, P.C. (1972) Biochemistry of the -SH group. Academic Press, London, New York

    Google Scholar 

  • Koretsky, A.P., Wang, S., Klein, H.P., James, T.L. and Weiner, H.W. (1986) Biochemistry 25, 77–84

    Article  Google Scholar 

  • Kosower, E.M., Correa, W., Kinon, B.J. and Kosower, N.S. (1972) Biochim. Biophys. Acta 264, 39–44

    Article  Google Scholar 

  • Kosower, N.S., Kosower, E.M., Newton, G.L. and Ranney, H.M. (1979) Proc. Natl. Acad. Sci. USA 76, 3382–3386

    Article  Google Scholar 

  • Krebs, H.A. and Henseleit, K. (1932) Z. Physiol. Chem. 210, 33–66

    Article  Google Scholar 

  • Lawson, J.W.R. and Veech, R.L. (1979) J. Biol. Chem. 254, 6528–6537

    Google Scholar 

  • Lippe, G., Dabbeni-Sala, F. and Sorgato, H.C. (1988) J. Biol. Chem. 263, 18627–18634

    Google Scholar 

  • Matthews, P.M., Bland, J.L., Gadian, D.G. and Radda, G.K. (1982) Biochim. Biophys. Acta 721, 312–320

    Article  Google Scholar 

  • Mela, L. and Seitz, S. (1979) In: Methods Enzymol. 55, pp. 39–46

    Article  Google Scholar 

  • Mitsos, S.E., Fantone, J.C., Gallagher, K.P., Waiden, K.M., Simpson, P.J., Abrams, G.D., Schork, N.A. and Lucchesi, B.R,. (1986) J. Cardiovasc. Pharm. 8, 978–988

    Article  Google Scholar 

  • Myers, M.L., Bolli, R., Lekich, R.F., Hartley, C.J. and Roberts, R. (1986) J. Am. Coll. Cardiol. 8, 161–1168

    Article  Google Scholar 

  • Neely, J.R., Lieberneister, B. and Morgan, H.E. (1967) Am. J. Physiol. 212, 815–822

    Google Scholar 

  • Neely, J.R. and Rovetto, U.J. (1975) In: Methods Enzymol. 39, pp. 43–60

    Article  Google Scholar 

  • Perry, S.B., McAuliffe, J., Baischi, J.A., Hickey, P.R. and Ingwall, J.S. (1988) Biochemistry 27, 165–2172

    Article  Google Scholar 

  • Pullman, M.E. (1967) In: Methods Enzymol. 10, pp. 57–60

    Article  Google Scholar 

  • Roldan, E.J.A., Kerzberg, E.M. and Boveris, A. (1989) Nedicina (Buenos Aires) 49, 336–340

    Google Scholar 

  • Ross, B.D. (1972) Perfusion Techniques in Biochemistry. A laboratory manual in the use of isolated perfused organs in biochemical experimentation. Clarendon Press, Oxford

    Google Scholar 

  • Saks, V.A., Cheraousova, G.B., Gukovsky, D.E., Smimow, V.N. and Chazov, E.I. (1975) Eur. J. Biochem. 57, 273–290

    Article  Google Scholar 

  • Searls, R.L. and Sanadi, D.R. (1960) J. Biol. Chem. 235, 2485–2491

    Google Scholar 

  • Serrano, R., Kanner, B. and Racker, E. (1976) J. Biol. Chem. 251, 2453–2461

    Google Scholar 

  • Spielmann, B., Jakob-Müller, U. and Schulz, P. (1981) Anal. Biochem. 113, 172–178

    Article  Google Scholar 

  • Taegtmeyer, H., Hems, R. and Krebs, B.A. (1980) BioChen. J. 186, 701–711

    Google Scholar 

  • Wyss, m., Schlegel, J., James, P., Eppenberger, H.M. and Wallimann, T. (1990) J. Biol. Chen. 265, 15900–15908

    Google Scholar 

  • Zimmer, G. (1977) Biochim. Bioshys. Acta 461, 268–273

    Article  Google Scholar 

  • Zimmer, G., Beyersdorf, F. and Fuchs, J. (1985) Mol. Physiol. 8, 495–513

    Google Scholar 

  • Zimmer, G. and Evers, J. (1988) Basic Res. Cardiol. 83, 445–451

    Article  Google Scholar 

  • Zimmer, G., Mainka, L. and Krüger, E. (1991) Arch. Biochem. Biophys. 288, 609–613

    Article  Google Scholar 

  • Zimmer, G., Mainka, L. and Ohlenschläger, G. (1978b) FEBS Letts. 94, 223–227

    Article  Google Scholar 

  • Zimmer, G., Schneider, M. and Hoffmann, H. (1978a) Arzneim. Forsch./Drug Res. 28, 811–816

    Google Scholar 

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Authors and Affiliations

  1. Gustav-Embden-Zentrum der Biologischen Chenie, Klinikum der Johann Wolfgang Goethe-Universität, Theodor-Stern-Kai 7, 6000, Frankfurt am Main, Germany

    H. J. Freisleben & G. Zimer

  2. Zentrum der Chirurgie, Klinikum der Johann Wolfgang Goethe-Universität, Theodor-Stern-Kai 7, 6000, Frankfurt am Main, Germany

    F. Beyersdorf, H. Assadnazari Seewald, J. Simon & A. Hanselmann

Authors
  1. H. J. Freisleben
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  2. F. Beyersdorf
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  3. H. Assadnazari Seewald
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  4. J. Simon
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  5. A. Hanselmann
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  6. G. Zimer
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Editors and Affiliations

  1. Malaysian Palm Oil Promotion Council, Bangunan Getah Asli 148 Jalan Ampang, 50450, Kuala Lumpur, Malaysia

    A. S. H. Ong

  2. Dept. of Molecular and Cell Biology 251 Life Science Addition, Membrane Bioenergetics Group, Berkeley, California, 94720, USA

    L. Packer

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© 1992 Birkhäuser Verlag, Basel/Switzerland

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Freisleben, H.J., Beyersdorf, F., Seewald, H.A., Simon, J., Hanselmann, A., Zimer, G. (1992). Dihydrolipoic Acid Is Protective Against Reperfusion Injury. In: Ong, A.S.H., Packer, L. (eds) Lipid-Soluble Antioxidants: Biochemistry and Clinical Applications. Molecular and Cell Biology Updates. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7432-8_41

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  • DOI: https://doi.org/10.1007/978-3-0348-7432-8_41

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-0348-7434-2

  • Online ISBN: 978-3-0348-7432-8

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