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Redox Mechanisms in T Cell Activation

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Oxidative Stress, Cell Activation and Viral Infection

Part of the book series: Molecular and Cell Biology Updates ((MCBU))

Summary

A variety of anti-oxidants, including free radical scavengers and iron chelators, inhibit the proliferation of mitogenstimulated T lymphocytes in vitro. These agents do not operate by inhibiting the induction of omithine decarboxylase activity. Phorbol myristate acetate induces formation of reactive oxygen species in T cell-enriched populations. Flow cytometry has identified this reactive oxygen within the T lymphocytes themselves, though other cells may contribute indirectly. The binding to DNA of the transcription factors AP-1 and NF-kB is increased during the commitment period, 2–4 hours after mitogen addition to T lymphocytes, and this binding is inhibited by the anti-oxidant aminothiol compound, cysteamine. Intracellular oxidant formation in mitogen-stimulated T cells might activate transcription factors, allowing their translocation into the nucleus and subsequent reduction and binding to DNA, thereby inducing early gene expression. Since the replication of the human immunodeficiency virus-1 is linked to NF-kB and can be inhibited by some anti-oxidants, it is possible that this virus is stimulated to replicate by the intracellular oxidative events that we have shown to be an obligatory step in T lymphocyte recruitment into the cell cycle.

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Authors and Affiliations

  1. Department of Pathology, University of Sydney, N.S.W., 2006, Australia

    N. H. Hunt, D. M. van Reyk, J. C. Fragonas, T. M. Jeitner & S. D. Goldstone

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  1. N. H. Hunt
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  2. D. M. van Reyk
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  3. J. C. Fragonas
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  4. T. M. Jeitner
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  5. S. D. Goldstone
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Editors and Affiliations

  1. INSERM U. 294, 16, rue Henri Huchard, F-75018, Paris, France

    C. Pasquier

  2. Dept. SIDA & Rétrovirus, Unité d’Oncologie Virale, 25-28, rue du Dr. Roux, F-75015, Paris, France

    R. Y. Olivier

  3. Institut Gustave Roussy, INSERM U. 140, 39, rue Camille Desmoulins, F-94805, Villejuif Cedex, France

    C. Auclair

  4. Dept. of Molecular and Cell Biology, 251 Life Science Addition, Membrane Bioenergetics Group, 94720, Berkeley, CA, USA

    Lester Packer

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© 1994 Birkhäuser Verlag Basel/Switzerland

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Hunt, N.H., van Reyk, D.M., Fragonas, J.C., Jeitner, T.M., Goldstone, S.D. (1994). Redox Mechanisms in T Cell Activation. In: Pasquier, C., Olivier, R.Y., Auclair, C., Packer, L. (eds) Oxidative Stress, Cell Activation and Viral Infection. Molecular and Cell Biology Updates. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7424-3_21

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  • DOI: https://doi.org/10.1007/978-3-0348-7424-3_21

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-0348-7426-7

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