Summary
Oxydative modification of human low density lipoproteins (LDL) is generally believed to initiate macrophage uptake by scavenger receptors which eventually leads to the formation of fatty streaks and atherosclerotic plaques that precede cardiovascular disease. Hence, sensitive methods to assess the existence of LDL subpopulations and knowledge about antioxidant prevention of LDL oxidative modifications are important. In this study, laser Doppler electrophoresis (LDE) was used to detect changes in electrophoretic mobility of LDL populations before and after exposure to oxidative stress. Before oxidative stress, LDL suspensions exhibited homogenous populations of net negative charge but after exposure to hydroxyl and superoxide radicals (simultaneously generated by steady state 60Cobalt gamma radiolysis), peroxyl radicals produced by the water-soluble azo-initiator 2,2′-azobis(2-amidinopropane)HC1 (AAPH), LDL populations exhibited mobility changes. The major population of LDL became more negatively charged in agreement with agarose gel electrophoresis (AGE). However, greater heterogeneity of LDL in some instances can be discerned by liquid as compared to solid phase electrophoresis. Hence, LDE is a sensitive method for detecting the appearance of subpopulations of LDL of differing surface charge density during oxidative modification. The effect of lipophilic antioxidants in protection against these changes in physicochemical properties of LDL was investigated. LDL obtained from a vitamin E deficient patient pretreated with β-carotene afforded protection. After exposure to peroxyl radicals generated by AAPH, a more narrow range of subpopulations was observed.Thus, β-carotene can protect LDL against oxidative modification even when endogenous vitamin E levels are low. In other experiments, with native LDL pretreated with betatene (an almost equivalent mixture of all-trans- and cis-ß-carotene), a protection was also observed.
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© 1994 Birkhäuser Verlag Basel/Switzerland
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Bonnefont-Rousselot, D., Arrio, B., Catudioc, J., Packer, L. (1994). Oxidization of human low density lipoproteins measured by Laser Doppler electrophoresis. In: Pasquier, C., Olivier, R.Y., Auclair, C., Packer, L. (eds) Oxidative Stress, Cell Activation and Viral Infection. Molecular and Cell Biology Updates. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7424-3_16
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DOI: https://doi.org/10.1007/978-3-0348-7424-3_16
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