Summary
Inhibition of cell proliferation by d-α-tocopherol occurs in vascular smooth muscle cells, Balb c/3T3 fibroblasts and neuroblastoma cells. Other cell lines, such as CHO, osteosarcoma and macrophages are not sensitive. The inhibition depends on the signalling path employed by cells to control proliferation. PDGF-BB is the most d-α-tocopherol sensitive mitogenic stimulus while lysophosphatidic acid is the least. d-β-Tocopherol, an analogue of d-α-tocopherol, with similar antioxidant properties, does not inhibit proliferation. Protein kinase C activity is inhibited by d-α-tocopherol but not by d-β-tocopherol, suggesting a central role of this enzyme in the control of cell proliferation by d-α-tocopherol.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Alexander, D. R., Hexham, J. M., Lucas, S. C., Graves, J. D., Cantrell, D. A. and Crumpton, M. J(1989) A protein kinase C pseudosubstrate peptide inhibits phosphorylation of the CD3 antigen in streptolysin-O-permeabilized human Tlymphocytes.Biochem. J.260: 893–901.
Ashihara, T. and Baserga, R. (1979) Cell synchronization. Methods Enzym. 58, 248–262
Azzi-A, Boscoboinik-D, Hensey-C. 1992 The protein kinase C family. Eur.J.Biochem. 208 547–57
Boscoboinik-D, Szewczyk-A, Azzi-A. (1991) Alpha—tocopherol (vitamin E) regulates vascular smooth muscle cell proliferation and protein kinase C activity. Arch.Biochem.Biophys., 286, 264–9
Boscoboinik-D, Szewczyk-A, Hensey-C, Azzi-A (1991) Inhibition of cell proliferation by atocopherol. Role of protein kinase C. J.Biol.Chem., 266, 6188–94
Burdon-R-H, Gill-V, Rice-Evans-C. (1990) Oxidative stress and tumour cell proliferation. Free Radic.Res.Commun., 11, 65–76
Burton-G-W, Ingold-K-U. 1989 Vitamin E as an in vitro and in vivo antioxidant. Ann.N.Y.Acad.Sci. 570, 7–22
Chen-L-H, Boissonneault-G-A, Glauert-H-P (1988) Vitamin C, vitamin E and cancer (review). Anticancer.Res., 8, 739–48
Clowes, A.W. ans Schwartz, S.M. (1985) Significance of quiescent smooth muscle migration in the injured rat carotid artery. Circ. Res. 156, 139–145
Hess-D, Keller-H-E, Oberlin-B, Bonfanti-R, Schuep-W. 1991 Simultaneous determination of retinol, tocopherols, carotenes and lycopene in plasma by means of high-performance liquid chromatography on reversed phase. Int.J.Vitam.Nutr.Res. 61, 232–8
Kraft-A-S, Anderson-W-B. 1983 Phorbol esters increase the amount of Ca2+, phospholipid dependent protein kinase associated with plasma membrane.Nature301, 621–3
Kuzuya M, Naito-M, Funaki-C, Hayashi-T, Yamada—K, Asai-K, Kuzuya—F. (1991) Antioxidants stimulate endothelial cell proliferation in culture. Artery, 18, 115–24
Mochly-Rosen-D, Khaner-H, Lopez-J, Smith-B-L 1991a Intracellular receptors for activated protein kinase C. Identification of a binding site for the enzyme. J.Biol.Chem. 266 14866–8
Mochly-Rosen-D, Khaner-H, Lopez-J. 199 lb Identification of intracellular receptor proteins foractivated protein kinase C. Proc.Natl.Acad.Sci.U.S;A. 88 3997–4000
Mochly-Rosen-D, Miller-K-G, Scheller-R-H, Khaner-H, Lopez-J, Smith-B- L. 1992 p65 fragments, homologous to the C2 region of protein kinase C, bind to the intracellular receptors for protein kinase C. Biochemistry 31 8120–4
Morisaki-N, Stitts-J-M, Bartels-Tomei-L, Milo-G-E, Panganamala—R-V, Cornwell-D-G. (1982) Dipyridamole: an antioxidant that promotes the proliferation of aorta smooth muscle cells. Artery 11, 88–107
Newman-M-J. (1990) Inhibition of carcinoma and melanoma cell growth by type 1 transforming growth factor beta is dependent on the presence of polyunsaturated fatty acids. Proc.Natl. Acad;Sci;U.S.A 87, 5543–7
Paganelli-G-M, Biasco-G, Brandi-G, Santucci-R, Gizzi-G, Villani-V, Cianci-M, Miglioli-M, Barbara—L. (1992) Effect of vitamin A, C, and E supplementation on rectal cell proliferation in patients with colorectal adenomas. J.Natl.Cancer.Inst 84, 47–51,.
Raines, E.W. and Ross, R. (1993). Smooth muscle cells and the pathogenesis of the lesion of atherosclerosis. Br. HeartJ. 69. 30S - 37S
Traber-M-G, Burton-G-W, Ingold-K-U, Kayden-H-J. 1990 RRR- and SRR-alpha-tocopherols are secreted without discrimination in human chylomicrons, but RRR-alpha-tocopherol is preferentially secreted in very low density lipoproteins./.Lipid.Res31 675–85
Traber-M-G, Sokol-R-J, Burton-G-W, Ingold-K-U, Papas-A-M, Huffaker-J- E, Kayden-HJ. 1990 Impaired ability of patients with familial isolated vitamin E deficiency to incorporate alpha-tocopherol into lipoproteins secreted by the liver.J.Clin.Invest85 397–407
Watson-R-R. (1986) Immunological enhancement by fat-soluble vitamins, minerals, and trace metals: a factor in cancer prevention. Cancer.Detect.Prev9, 67–77
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Birkhäuser Verlag Basel/Switzerland
About this chapter
Cite this chapter
Azzi, A., Boscoboinik, D., Chatelain, E., Özer, N.K., Stäuble, B. (1994). d-α-Tocopherol and Cell Proliferation. In: Pasquier, C., Olivier, R.Y., Auclair, C., Packer, L. (eds) Oxidative Stress, Cell Activation and Viral Infection. Molecular and Cell Biology Updates. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7424-3_13
Download citation
DOI: https://doi.org/10.1007/978-3-0348-7424-3_13
Publisher Name: Birkhäuser Basel
Print ISBN: 978-3-0348-7426-7
Online ISBN: 978-3-0348-7424-3
eBook Packages: Springer Book Archive