Summary
The human 32kDa stress protein is synthesised following cellular redox imbalance and has been identified as a specific marker of oxidative stress; 32kDa stress protein-inducing agents include heavy metals, oxidising species and sulphydryl reactive compounds. As well as inducing the 32kDa protein, the oxidant H2O2 has been shown to induce transcription of the immediate-early genes and this activation is serum dependent. Our evidence that hypoxia/ reperfusion events occur in mobile inflamed joints prompted us to examine the vasculature. Therefore, human umbilical vein endothelial cells (HUVEC) were challenged with cadmium chloride, hydrogen peroxide (H2O2) and disodium aurothiomalate in the presence and absence of serum. The results show that a 32kDa protein can be induced in HUVEC, that the response to H2O2 is serum dependent and that it occurs at the level of transcription. Thus, two mechanisms of induction are possible: oxidants (eg H2O2) require serum while heavy metals and sulphydryl-reactive compounds may use a different pathway.
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References
Allen, R.E., Blake, D.R., Nazhat, N.B., and Jones, P. (1989) Superoxide radical generation by inflamed human synovium after hypoxia. Lancet 2: 282–283.
Amstad, P.A., Krupitza, G., and Cerutti, P.A. (1992) Mechanism of c-fos induction by active oxygen. Cancer Res 52: 3952–3960.
Applegate, L.A., Luscher, P., and Tyrrell, R.M. (1991) Induction of heure oxygenase: A general response to oxidant stress in cultured mammalian cells. Cancer Res 51: 974–978.
Blake, D.R., Merry, P., Unsworth, J., Kidd, B.L., Outhwaite, J.M., Ballard, R., Morris, C.J., Gray, L., and Lunec, J. (1989) Hypoxic-reperfusion injury in the inflamed human joint. Lancet 1: 289–293.
Burdon, R.H., Gill, V.M., and Rice-Evans, C. (1987) Oxidative stress and heat shock protein induction in human cells. Free Radic Res Commun 3: 129–139.
Caltabiano, M.M., Koestler, T.P., Poste, G., and Greig, R.G. (1986) Induction of 32- and 34-kDa stress proteins by sodium arsenite, heavy metals, and thiol-reactive agents. J Biol Chem 261: 13381–13386.
Caltabiano, M.M., Poste, G., and Greig, R.G. (1988) Induction of the 32-kD human stress protein by auranofin and related triethylphosphine gold analogs. Biochem Pharmacol 37: 4089–4093.
Carper, S.W., Duffy, J.J., and Gerner, E.W. (1987) Heat shock proteins in thermotolerance and other cellular processes. Cancer Res 47: 5249–5255.
Chomczynski, P., and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159.
Cohen, I.R. (1991) Autoimmunity to chaperonins in the pathogenesis of arthritis and diabetes. Annu Rev Immunol 9: 567–589.
Demolle, D., Lecompte, M., and Boeynaems, J-M. (1988) Pattern of protein phosphorylation in aortic endothelial cells. Modulation by adenine nucleotides and bradykinin. J Biol Chem 263: 18459–18465.
Donati, Y.R., Slosman, D.O., and Polla, B.S. (1990) Oxidative injury and the heat shock response. Biochem Pharmacol 40: 2571–2577.
Ellis, R.J., and van der Vies, S.M. (1991) Molecular chaperones. Annu Rev Biochem 60: 321347.
Ferris, D.K., Harel-Bellan, A., Morimoto, R.I., Welch, W.J., and Farrar, W.L. (1988) Mitogen and lymphokine stimulation of heat shock proteins in T lymphocytes. Proc Natl Acad Sci USA 85: 3850–3854.
Jaffe, E.A., Nachmann, R.L., Becker, C.G., and Minick, C.R. (1973) Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 52: 2745–2756.
Jornot, L., Mirault, M.E., and Junod, A.F. (1987) Protein synthesis in hyperoxic endothelial cells: Evidence for translational defect. J Appl Physiol 63: 457–464.
Jornot, L., Mirault, M.E., and Junod, A.F. (1991) Differential expression of hsp70 stress proteins in human endothelial cells exposed to heat shock and hydrogen peroxide. Am J Respir Cell Mol Biol 5: 265–275.
Kantengwa, S., and Polla, B.S. (1991) Flavonoids, but not protein kinase C inhibitors, prevent stress protein synthesis during erythrophagocytosis. Biochem Biophys Res Commun 180: 308–314.
Kaur, P., Welch, W.J., and Saklatvala, J. (1989) Interleukin 1 and tumour necrosis factor increase phosphorylation of the small heat shock protein. Effects in fibroblasts, Hep G2 and U937 cells. FEBS Lett 258: 269–273.
Ketis, N.V., Hoover, R.L., and Karnovsky, M.J. (1989) Effects of hyperthermia on cell survival and patterns of protein synthesis in endothelial cells from different origins. Cancer Res 48: 2101–2106.
Keyse, S.M., and Tyrrell, R.M. (1989) Herne oxygenase is the major 32-kDa stress protein induced in human skin fibroblasts by UVA radiation, hydrogen peroxide, and sodium arsenite. Proc Natl Acad Sci USA 86: 99–103.
Keyse, S.M., and Tyrrell, R.M. (1990) Induction of the heme oxygenase gene in human skin fibroblasts by hydrogen peroxide and UVA (365nm) radiation: Evidence for the involvement of the hydroxyl radical. Carcinogenesis (Lond) 11: 787–791.
Keyse, S.M., Applegate, L.A., Tromvoukis, Y., and Tyrrell, R.M. (1990) Oxidant stress leads to transcriptional activation of the human heme oxygenase gene in cultured skin fibroblasts. Mol Cell Biol 10: 4967–4969.
Koizumi, T., Yamauchi, R., Irie, A., Negishi, M., and Ichikawa, A. (1991) Induction of a 31,000-Dalton stress protein by prostaglandins D2 and J2 in porcine aortic endothelial cells. Biochem Pharmacol 42: 777–785.
Kurata, S-I., and Nakajima, H. (1990) Transcriptional activation of the heme oxygenase gene by TPA in mouse M1 cells during their differentiation to macrophage. Exp Cell Res 191: 89–94.
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.
Latchman, D.S. (1991) Heat shock proteins and human disease. J R Coll Physicians Lond 25: 295–299.
Levinson, W., Oppermann, H., and Jackson, J. (1980) Transition series metals and sulfhydryl reagents induce the synthesis of four proteins in eukaryotic cells. Biochim Biophys Acta 606: 170–180.
Lindquist, S. (1986) The heat-shock response. Annu Rev Biochem 55: 1151–1191.
Lunec, J., Brailsford, S., Hewitt, S.D., Morris, C.J., and Blake, D.R. (1987) Free radicals: Are they possible mediators of IgG denaturation and immune complex formation in rheumatoid arthritis. Int J Immunotherapy 3: 39–43.
Maines, M.D. (1988) Herne oxygenase: Function, multiplicity, regulatory mechanisms, and clinical applications. FASEB J 2: 2557–68.
Mitani, K., Fujita, H., Sassa, S., and Kappas, A. (1990) Activation of heme oxygenase and heat shock protein 70 genes by stress in human hepatoma cells. Biochem Biophys Res Commun 166: 1429–1434.
Morimoto, R.I. (1993) Cells in stress: Transcriptional activation of heat shock genes. Science 259: 1409–1410.
Nose, K., Shibanuma, M., Kikuchi, K., Kageyama, H., Sakiyama, S., and Kuroki, T. (1991) Transcriptional activation of early-response genes by hydrogen peroxide in a mouse osteoblastic cell line. Eur J Biochem 201: 99–106.
Polla, B.S. (1988) A role for heat shock proteins in inflammation? Immunol Today 9: 134–137.
Riabowol, K.T., Mizzen, L.A., and Welch, W.J. (1988) Heat shock is lethal to fibroblasts microinjected with antibodies against hsp70. Science 242: 433–436.
Stevens, C.R., Williams, R.B., Farrell, A.J., and Blake, D.R. (1991) Hypoxia and inflammatory synovitis: Observations and speculation. Ann Rheum Dis 50: 124–132.
Subjeck, J.R., and Shyy, T.T. (1986) Stress protein systems of mammalian cells. Am J Physiol 250: C1 - C17.
van Eden, W. (1991) Heat-shock proteins as immunogenic bacterial antigens with the potential to induce and regulate autoimmune arthritis. Immunol Rev 121: 5–28.
Welch, W.J. (1990) The response of mammalian cells to environmental stress. In: Burdon, R., Rice-Evans C., Blake, D. and Winrow, V. (eds.): Stress Proteins in Inflammation, Richelieu Press, London, pp. 13–52.
Welch, W.J. (1992) Mammalian stress response: Cell physiology, structure/function of stress proteins, and implications for medicine and disease. Physiol Rev 72: 1063–1081.
Winrow, V.R., and Blake, D.R. (1991) Stress proteins, hearts, and joints. Lancet 337: 614–615.
Winrow V.R., McLean, L., Morris, C.J., and Blake, D.R. (1990) The heat shock response and its role in inflammatory disease. Ann Rheum Dis 49: 128–132.
Winrow, V.R., Mojdehi, G., Mapp, P.I., Rampton, D.S., and Blake, D.R. (1990) Immunohistological localisation of stress proteins in inflammatory tissue. In: Burdon, R., Rice-Evans C., Blake, D. and Winrow, V. (eds.): Stress Proteins in Inflammation, Richelieu Press, London, pp. 237–51.
Winyard, P.G., Zhang, Z., Chidwick, K., Blake, D.R., Carrell, R.W., Murphy, G. (1991) Proteolytic inactivation of human 1 antitrypsin by human stromelysin. FEBS Lett 279: 91–94.
Young, D., Lathigra, R., Hendrix, R., Sweetser, D., and Young, R.A. (1988) Stress proteins are immune targets in leprosy and tuberculosis. Proc Natl Acad Sci USA 85: 4267–4270.
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Winrow, V.R., Watson, A., Harley, S.L., Blake, D.R. (1994). Hydrogen peroxide-induced synthesis of the 32kDa stress protein (HO-1) in endothelial cells is serum dependent. In: Pasquier, C., Olivier, R.Y., Auclair, C., Packer, L. (eds) Oxidative Stress, Cell Activation and Viral Infection. Molecular and Cell Biology Updates. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7424-3_10
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DOI: https://doi.org/10.1007/978-3-0348-7424-3_10
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