Abstract
The glycosaminoglycans (GAGs) are a family of complex linear polysaccharides characterized by a repeating core disaccharide structure comprising of an N-substituted hexosamine and, usually, a uronic acid. They can be usefully categorized into four structural groups: hyaluronate (HA); chondroitin sulphate (CS)/dermatan sulphate (DS); heparan sulphate (HS)/heparin and keratan sulphate (KS). HA possesses a completely invariant repeat sequence and is therefore not considered further. KS is somewhat unusual in containing a neutral sugar rather than a uronate residue. It may also possess a small proportion of branched fucose residues as well as terminal capping sialic acids. The structural analysis of KS is comprehensively discussed in chapter 9. This article will concentrate on disaccharide analyses of the remaining two complex groups: CS/DS and HS/heparin. The N-acetylated galactosaminoglycans CS and DS differ in the exclusive presence of glucuronate (GlcA) in CS, whilst in DS a variable proportion of the G1cA residues are epimerized to iduronate (IdoA). Potential O-sulphations at C-4/C-6 of GalNAc and C-2 of IdoA provide for a further level of complexity. However, the most complex GAGs are undoubtedly the glucosaminoglycans HS and heparin, which experience postpolymeric modifications of both the monosaccharide constituents of the original disaccharide repeat (for review see ref.1). G1cA residues can be epimerized to IdoA, whilst GlcNAc residues can be simultaneously de-N-acetylated/re-N-sulphated yielding N-sulphoglucosamine (GlcNSO3). Both these conversions occur to a greater extent in heparin than in HS.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Lindahl U (1989) Biosynthesis of Heparin and Related Polysaccharides. In: D. A. Lane and U. Lindah (eds) Heparin, p. 159, Edward Arnold, London.
Lyon M, Deakin JA and Gallagher JT (1994) J. Biol Chem. 269: 1 1208.
Kato M, Wang H, Bernfield M, Gallagher JT and Turnbull JE (1994) J. Biol Chem. 269: 18881.
Turnbull JE, Fernig DG, Ke Y, Wilkinson MC and Gallagher JT (1992) J. Biol Chem. 267: 10337.
Lyon M, Deakin JA, Mizuno K, Nakamura T and Gallagher JT (1994) J. Biol Chem. 269: 1 1216.
Yoshida K, Miyauchi S, Kikuchi H, Tawada A and Tokuyasu K (1989) Anal Biochem. 177: 327.
Desai UR, Wang H-M and Linhardt RJ (1993) Biochemistry 32: 8140.
Yamada S, Sakamoto K, Tsuda H, Yoshida K, Sugahara K, Khoo K-H, Morris HR and Dell A (1994) Glycobiology 4: 69.
Yamada S, Yoshida K, Sugiura M, Sugahara K, Khoo K-H, Morris HR and Dell A (1993) J. Biol Chem. 268: 4780.
Shaklee PN and Conrad HE (1986) Biochem. J. 235: 225.
Guo Y and Conrad HE (1989) Anal Biochem. 176: 96.
Lohse DL and Linhardt RJ (1992) J. Biol Chem. 267: 24347.
Kusche M, Hannesson HH and Lindahl U (1991) Biochem. J. 275: 151.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Birkhäuser Verlag Basel
About this chapter
Cite this chapter
Lyon, M. (1997). Determination of the Disaccharide Composition of Glycosaminoglycans: Comparison of Chemical and Enzymatic Scission. In: Jackson, P., Gallagher, J.T. (eds) A Laboratory Guide to Glycoconjugate Analysis. BioMethods. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7388-8_4
Download citation
DOI: https://doi.org/10.1007/978-3-0348-7388-8_4
Publisher Name: Birkhäuser Basel
Print ISBN: 978-3-0348-7390-1
Online ISBN: 978-3-0348-7388-8
eBook Packages: Springer Book Archive