Summary
Methods are described for the preparation of poly(ADP-ribose) and for the preparation of biotinylated poly(ADP-ribose). Poly(ADP-ribose) is covalently modified to its biotinylated derivative by light-induced reaction of photobiotin with the polymer. Furthermore, conditions are given for the detection of poly(ADP-ribose) binding proteins in ligand-blotting experiments. The electrophoretically separated proteins are identified on blots using biotinylated poly(ADP-ribose) in a staining reaction. The results obtained with biotinylated poly(ADP-ribose) are comparable with those obtained with radioactive poly(ADP-ribose).
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© 1996 Birkhäuser Verlag
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Narendja, F.M., Sauermann, G. (1996). Biotin-Labeling of Poly(ADP-ribose) in Poly(ADP-ribose)-Protein Interactions. In: Meier, T., Fahrenholz, F. (eds) A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis. BioMethods. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7349-9_8
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DOI: https://doi.org/10.1007/978-3-0348-7349-9_8
Publisher Name: Birkhäuser Basel
Print ISBN: 978-3-0348-7351-2
Online ISBN: 978-3-0348-7349-9
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