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Tea and its components as powerful antioxidants

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Oxidative Stress and Aging

Part of the book series: Molecular and Cell Biology Updates ((MCBU))

Summary

The administration of tea polyphenol (TPP) diet containing 1.0% TPP to rats for 18 months significantly suppressed the increase of TBARS level in the plasma. The levels of total cholesterol (TC) and phospholipids (PL) in the plasma were also lowered. Positive correlations existed between the TBARS and triglyceride (TG), TC or PL levels in the plasma of rats 12 months after the feeding of TPP diet.

TPP especially, (-)-epigallocatechin gallate (EGCG) and (-)-epicatechin gallate (ECG) were much more effective than BHT, vitamin E or C in inhibiting the Cu2+ mediated oxidative modification of low density lipoprotein (LDL). EGCG strongly inhibited cholesterol ester oxidation and apolipoprotein B-100 fragmentation of LDL.

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References

  • Akasaka, K., Suzuki, T., Ohrui, H. and Meguro, H. (1987a) Study on aromatic phosphines for novel fluorometry of hydroperoxides I: Synthesis and spectral properties of diphenyl aryl phosphines and their oxides. Anal. Lett. 20: 731–745.

    Article  CAS  Google Scholar 

  • Akasaka, K., Suzuki, T., Ohrui, H. and Meguro, H. (1987b) Study on aromatic phosphines for novel fluorometry of hydroperoxides II: The determination of lipid hydroperoxides with diphenyl-1-pyrenylphosphine. Anal. Len. 20: 797–807.

    Article  CAS  Google Scholar 

  • Bligh, E.G., and Dyer, W.J. (1959) A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37: 911–917.

    Article  PubMed  CAS  Google Scholar 

  • Esterbauer, H., Gebicki, J., Puhl, H. and JĂĽrgens, G. (1992) The role of lipid peroxidation and antioxidants in oxidative modification of LDL. Free Rad. Biol. Med. 13: 341–390.

    Article  PubMed  CAS  Google Scholar 

  • Hamberg, M. and Samuelsson, B. (1967) On the specificity of the oxygenation of unsaturated fatty acids catalyzed by soybean lipoxidase. J. Biol. Chem. 242: 5329–5335.

    PubMed  CAS  Google Scholar 

  • Havel, R.J., Eder, H.A. and Bragdon, J.H. (1955) Distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J. Clin. Invest. 34: 1345–1353.

    Article  PubMed  CAS  Google Scholar 

  • Lowry, O.H., Rosebrough, N.J., Fan, A.L. and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275.

    PubMed  CAS  Google Scholar 

  • Mangiapane, H., Thomson, J., Salter, A., Brown, S., Bell, G.D. and White, D.A. (1992) The inhibition of the oxidation of low density lipoprotein by (+)-catechin, a naturally occurring flavonoid. Biochem. Pharmacol. 43: 445–450.

    Article  PubMed  CAS  Google Scholar 

  • Masugi, F. and Nakamura, T. (1977) Measurement of thiobarbituric acid value in liver homogenate solubilized with sodium dodecylsulphate and variation of the values affected by vitamin E and drugs. Vitamins 51: 21–29.

    CAS  Google Scholar 

  • Matsuzaki, T. and Hara, Y. (1985) Antioxidative activity of tea leaf catechins. Nippon Nogeikagaku Kaishi 59: 129–134.

    Article  CAS  Google Scholar 

  • Nishioka, I. (1991) Tea polyphenol. In: K. Muramatsu (ed.): Science of Tea, Asakura Shoten, Tokyo, pp 115–123.

    Google Scholar 

  • Ohkawa, H., Ohishi, N. and Yagi, K. (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95: 351–358.

    Article  PubMed  CAS  Google Scholar 

  • Okuda, T., Kimura, Y., Yoshida, T., Hatano, T., Okuda, H. and Arichi, S. (1983) Studies on the activities of tannins and related compounds from medicinal plants and drugs I: Inhibitory effects on lipid peroxidation in mitochondria and microsomes of liver. Chem. Pharm. Bull. 31: 1625–1631.

    Article  PubMed  CAS  Google Scholar 

  • Sano, M., Abe, M., Yoshino, K., Matsuura, T., Sekino, T., Saito, S. and Tomita, I. (1986) A simple and convenient method to determine the activities of antioxidants using a-methylindole reagent and high-performance thin-layer chromatography. Chem. Pharm. Bull. 34: 174–178.

    Article  CAS  Google Scholar 

  • Sano, M., Takahashi, Y., Komatsu, C., Nakamura, Y., Shimoi, K., Tomita, I., Oguni, I., Nasu, K., Obata, K., Konomoto, H., Masuzawa, T. and Suzuki, N. (1991) Antioxidative activities of green tea and black tea in rat organ and blood plasma. In: Proceedings of the International Symposium on Tea Science (Shizuoka), Kurofune Print. Co., Shizuoka, pp 304–308.

    Google Scholar 

  • Steinberg, D. and Witztum, J.L. (1990) Lipoproteins and atherogenesis: Current concepts. JAMA (J. Am. Med. Assoc.) 264: 3047–3052.

    Article  CAS  Google Scholar 

  • Tanizawa, H., Toda, S., Sazuka, Y., Taniyama, T., Hayashi, T., Arichi, S. and Takino, Y. (1984) Natural antioxidants I: Antioxidative components of tea leaf (Thea sinensis L.). Chem. Pharm. Bull. 32: 2011–2014.

    Article  PubMed  CAS  Google Scholar 

  • de Whalley, C.V., Rankin, S.M., Hoult, J.R.S., Jessup, W. and Leake, D.S. (1990) Flavonoids inhibit the oxidative modification of low density lipoproteins by macrophages. Biochem. Pharmacol. 39 (11): 1743–1750.

    Article  PubMed  Google Scholar 

  • Witztum, J.L. and Steinberg, D. (1991) Role of oxidized low density lipoprotein in atherogenesis. J. Clin. Invest. 88: 1785–1792.

    Article  PubMed  CAS  Google Scholar 

  • Yagi, K. (1976) A simple fluorometric assay for lipoperoxide in blood plasma. Biochem. Med. 15: 212–216.

    Article  PubMed  CAS  Google Scholar 

  • Yoshino, K., Hara, Y., Sano, M. and Tomita, I. (1994) Antioxidative effects of black tea theaflavins and thearubigin on lipid peroxidation of rat liver homogenates induced by tert-butyl hydroperoxide. Biol. Pharm. Bull. 17 (1): 146–149.

    Article  PubMed  CAS  Google Scholar 

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© 1995 Birkhäuser Verlag Basel/Switzerland

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Tomita, I. et al. (1995). Tea and its components as powerful antioxidants. In: Cutler, R.G., Packer, L., Bertram, J., Mori, A. (eds) Oxidative Stress and Aging. Molecular and Cell Biology Updates. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7337-6_33

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  • DOI: https://doi.org/10.1007/978-3-0348-7337-6_33

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-0348-7339-0

  • Online ISBN: 978-3-0348-7337-6

  • eBook Packages: Springer Book Archive

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