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Antioxidant genes and other mechanisms involved in the extended longevity of Drosophila

  • Conference paper
Oxidative Stress and Aging

Part of the book series: Molecular and Cell Biology Updates ((MCBU))

Summary

We are engaged in a genetic analysis of the mechanisms responsible for the expression of the extended longevity phenotype (ELP) of Drosophila. This phenotype is characterized by a ca. 40% increase in mean and maximum life span. We have shown that the ELP is due to a delayed onset of senescence (DOS) in one of our selected strain (LA) relative to its control (RA) stain. A complete genetic analysis, identifying the role of each chromosome in the expression of the ELP in the LA strain, has been done. The recessive genes responsible have been localized to the 3rd chromosome (c3). Genes on other chromosomes are involved in the expression of the ELP but are not expressed in the absence of the c3 genes. There are epistatic effects as well. In addition, expression of the ELP requires raising the L animals under high larval density (HD) conditions; raising the same genotype under low density (L-LD) conditions leads to a normal life span. There exists a critical period in larval life during which the L-HD animal programs the expression of genes in the young adult. This takes the form of a coordinated increase in specific antioxidant mRNA levels and enzyme activities in the 5 day old L-HD adult, a time point which just precedes the DOS. These increases are functional, as shown by the fact that the L-HD animals show an increase in their resistance to exogenous paraquat at this same time. The control (R-HD and L-LD) strains do not show such elevations and shortly thereafter show a decrease in their paraquat resistance and other biomarkers which signal the normal onset of senescence. Destruction of catalase activity in L-HD animals results in a failure to express the paraquat resistance component of the ELP. Thus, the critical period events which take place in the larva lead to coordinated changes in antioxidant gene activities in the young adult, and bring about the DOS and the expression of the ELP. These events do not appear to be limited to one strain. Our data shows that the only stress factor or biochemical trait which distinguishes all of our independently derived L strains from their related control and short-lived strains is their enhanced resistance to exogenous paraquat. The down-regulation of the antioxidant defense gene system must be viewed as the proximal cause of the onset of senescence.

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References

  • Arking, R. (1987) Successful selection for increased longevity in Drosophila: Analysis of the survival data and presentation of a hypothesis on the genetic regulation of longevity. Exp. Gerontol.22:199–220.

    Google Scholar 

  • Arking, R., Buck, S.A., Wells, R.A. and Pretzlaff, R. (1988) Metabolic rates in genetically based long-lived strains of Drosophila. Exp. Gerontol. 23: 59–76.

    Article  CAS  Google Scholar 

  • Arking, R. and Dudas, S.P. (1989) A review of genetic investigations into aging processes of Drosophila. J. Amer. Geriatr. Soc. 37:757–773.

    Google Scholar 

  • Arking, R. and Wells, R.A. (1990) Genetic alteration of normal aging processes is responsible for extended longevity in Drosophila. Dev. Gen. 11: 141–148.

    CAS  Google Scholar 

  • Arking, R., Buck, S.A., Berrios, A., Dwyer, S. and Baker, G.T.III (1991) Elevated paraquat resistance can be used as a bioassay for longevity in a genetically based long—lived strain of Drosophila. Dev. Genet. 12: 362–370.

    CAS  Google Scholar 

  • Arking, R., Dudas, S.P. and Baker, G.T.III (1993a) Genetic and environmental factors regulating the expression ofan extend ed longevity phenotype in a long-lived strain of Drosophila. Genetica 91:127–142.

    Google Scholar 

  • Arking, R., Dudas, S.P. and Baker, G.T.III (1993a) Genetic and environmental factors regulating the expression ofan extend ed longevity phenotype in a long-lived strain of Drosophila. Genetica 91:127–142.

    Google Scholar 

  • Baret, P. and Lints, F.A. (1993) Selection for increased longevity in Drosophila melanogaster: A new interpretation. Gerontology 39:252–259.

    Google Scholar 

  • Buck, S., Wells, R.A., Dudas, S.P., Baker, G.T.III and Arking, R. (1993a) Chromosomal localization and regulation of the longevity determinant genes in a selected strain of Drospohila melanogaster. Heredity 71:11–22.

    Google Scholar 

  • Buck, S., Nicholson, M., Dudas, S.P., Baker, G.T.III and. Arking, R. (1993b) Larval regulation of adult longevity in a genetically selected long-lived strain of Drosophila melanogaster. Heredity 71:23–32.

    Google Scholar 

  • Dudas, S.P. and Arking, R. (1994) The expression of the EF1 genes of Drospohila is not associated with the extended longevity phenotype in a selected long-lived strain. Exp. Gerontol. 29: 645–657.

    Google Scholar 

  • Dudas, S.P. and Arking, R. (1994) The expression of the EF1 genes of Drospohila is not associated with the extended longevity phenotype in a selected long-lived strain. Exp. Gerontol. 29: 645–657.

    Google Scholar 

  • Force, A.G., Staples, T. and Arking, R. (1993) Longevity in independent derived long-lived strains of Drosophila is correlated with different suites of physiological characters. Gerontologist 33:168 (abstr.).

    Google Scholar 

  • Force, A.G., Staples, T., Soliman, S. and Arking, R. (1995) A comparative biochemical and stress analysis of genetically selected Drospohila strains with different longevities.; (submitted).

    Google Scholar 

  • Graves, J.L., Toolson, E.C., Jeong, C., Vu, L.N. and Rose, M.R. (1992) Dessication, flight, glycogen, and postponed senescence in Drospohila melanogaster. Physiol. Zool. 65: 268–286.

    CAS  Google Scholar 

  • Hovemann, B., Richter, S., Walldorf, U. and Cziepluch, C. (1988) Two genes encode related cytoplasmic elongation factors la (EF-1a) in Drosophila melanogaster with continuous and stage specific expression. Nucleic Acids Res. 16 (8): 3175–3194.

    Article  PubMed  CAS  Google Scholar 

  • Johnson, T.E. (1988) Minireview: Genetic specification of life span: Processes, problems, and potentials. J. Gerontol. Biol. Sci. 43(4):B87–92.

    Google Scholar 

  • Lints, F.A. and Hoste, C. (1974) The Lansing effect revisited. I. Life span. Exp. Genotol. 9:51–69.

    Google Scholar 

  • Luckinbill, L.S., Arking, R., Clare, M.J., Cirocco, W.C. and Buck, S.A. (1984) Selection for delayed senescence in Drosophila melanogaster. Evol. 38:996–1004.

    Google Scholar 

  • Luckinbill, L.S., Clare, M.J., Krell, W.L., Cirocco, W.C. and Richards, P.A. (1987) Estimating the number of genetic elements that defer senescence in Drospohila. Evol. Ecol. 1: 37–46.

    Google Scholar 

  • Luckinbill, L.S., Graves, J.L., Reed, A.H. and Koetsawang, S. (1988) Localizing genes that defer senescence in Drospohila melanogaster. Heredity 60:367–374.

    Google Scholar 

  • Mackay, W.J. and Bewley, G.C. (1989) The genetics of catalase in Drosophila melanogaster: Isolation and characterization of acatalasemic mutants. Genetics 122:643–652.

    Google Scholar 

  • Makrides, S.C. (1983) Protein synthesis and degradation. Biol. Rev. 58: 344–422.

    Google Scholar 

  • Mayr, E. (1961) Cause and effect in biology. Science 134: 1501–1506.

    Google Scholar 

  • Orr, W.C. and Sohal, R.J. (1994) Extension of life-span by overexpression of superoxide dismutase and catalase in Drosophila melanogaster. Science 263:1128–1130.

    Google Scholar 

  • Pearl, R. (1928) The Rate of Living. University of London Press, London.

    Google Scholar 

  • Phillips, J.P. and Hilliker, A.J. (1990) Genetic analysis of oxygen defense mechanisms in Drosophila melanogaster. Adv. Genet. 28:43–71.

    Google Scholar 

  • Rose, M.R. (1991) Evolutionary Biology of Aging. Oxford University Press, New York.

    Google Scholar 

  • Shepherd, J.C.W., Walldorf, U., Hug, P. and Gehring, W.J. (1989) Fruit flies with additional expression of the elongation factor EF-la live longer. Proc. Natl. Acad. Sci. USA 86: 7520–7521.

    Article  PubMed  CAS  Google Scholar 

  • Service, P.M. (1987) Physiological mechanisms of increased stress resistance in Drosophila melanogaster selected for postponed senescence. Physiol. Zool. 60(3):321–326.

    Google Scholar 

  • Soliman, S. and Arking, R. (1994) Antioxidant gene activity patterns and oxidative damage in short-lived and long-lived strains of Drosophila. Gerontologist 34: 13.

    Google Scholar 

  • Walldorf, U., Hovemann, B. and Bautz, E.K.F. (1985) Fl and F2: Two similar genes regulated differently during development of Drosophila melanogaster. Proc. Natl. Acad. Sci. USA 82: 5795–5799.

    Article  CAS  Google Scholar 

  • Antioxidant genes and other mechanisms involved in the extended longevity of Drosophila 139

    Google Scholar 

  • Webster, G.C. (1986) Effects of aging on the components of the protein synthesis system. In: K.G. Collatz and R.S. Sohal (eds): Insect Aging. Springer-Verlag, Berlin-Heidelberg, pp 207–216.

    Google Scholar 

  • Wells, R.A., Buck, S. Ali, R. Marzouq, O. and Arking, R. (1987) Localization of the longevity genes in D. melanogaster. Gerontologist 27: 149A.

    Google Scholar 

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Authors and Affiliations

  1. Dept. of Biological Sciences and Institute of Gerontology, Wayne State University, Detroit, MI, 48202, USA

    R. Arking

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  1. R. Arking
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Editor information

Editors and Affiliations

  1. Gerontology Research Center, National Institute on Aging, NIH, 21224, Baltimore, MD, USA

    R. G. Cutler

  2. Dept. Molecular and Cell Biology, 251 Life Science Addition, Membrane Bioenergetics Group, 94720, Berkeley, CA, USA

    L. Packer

  3. Cancer Research Center of Hawaii, Molecular Oncology, University of Hawaii at Manoa, 1236 Lauhala Street, 96813, Honolulu, HI, USA

    J. Bertram

  4. Dept. of Neuroscience, Institute of Cellular and Molecular Medicine, Okayama University, 2-5-1 Shikatacho, Okayama 700, Japan

    A. Mori

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© 1995 Birkhäuser Verlag Basel/Switzerland

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Arking, R. (1995). Antioxidant genes and other mechanisms involved in the extended longevity of Drosophila . In: Cutler, R.G., Packer, L., Bertram, J., Mori, A. (eds) Oxidative Stress and Aging. Molecular and Cell Biology Updates. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7337-6_14

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  • DOI: https://doi.org/10.1007/978-3-0348-7337-6_14

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-0348-7339-0

  • Online ISBN: 978-3-0348-7337-6

  • eBook Packages: Springer Book Archive

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